"
Team:TU Eindhoven/Design/Plasmid Design
From 2014.igem.org
(Difference between revisions)
Rafiqlubken (Talk | contribs) |
|||
| (5 intermediate revisions not shown) | |||
| Line 127: | Line 127: | ||
<div class="col_700_2 float_r"> | <div class="col_700_2 float_r"> | ||
<h2>Plasmid Design</h2> | <h2>Plasmid Design</h2> | ||
| - | <p>The chosen vector for expression is pET29a. | + | <p>The chosen vector for expression is pET29a(+). The pET29a(+) vector has a Kanamycin resistance gene. The expression of an inserted gene is induced by IPTG. In both anchors at the N- and C-terminus the restriction sites for NdeI and SacI were introduced to clone the genes into a pET29a(+) vector. The modified genes were first digested with the restriction enzymes and so was the pET29a(+) vector. (<a href='#Fig1'>Figure 1</a> and <a href='#Fig2'>Figure 2</a>) After digestion the genes were ligated into the vector. For the protocol see: <a href="https://static.igem.org/mediawiki/2014/3/35/TU_Eindhoven_Protocol_Insert_%2B_Vector_Ligation.pdf" target="_blank">Vector Ligation</a> </p> |
<img id='Fig1' src="https://static.igem.org/mediawiki/2014/1/1e/TU_Eindhoven_PET29a_COMPx.png" class="image_wrapper image_fr" width="1085"> | <img id='Fig1' src="https://static.igem.org/mediawiki/2014/1/1e/TU_Eindhoven_PET29a_COMPx.png" class="image_wrapper image_fr" width="1085"> | ||
Latest revision as of 00:57, 18 October 2014
Plasmid Design
The chosen vector for expression is pET29a(+). The pET29a(+) vector has a Kanamycin resistance gene. The expression of an inserted gene is induced by IPTG. In both anchors at the N- and C-terminus the restriction sites for NdeI and SacI were introduced to clone the genes into a pET29a(+) vector. The modified genes were first digested with the restriction enzymes and so was the pET29a(+) vector. (Figure 1 and Figure 2) After digestion the genes were ligated into the vector. For the protocol see: Vector Ligation
Figure 1. Plasmid design pET29a(+) COMPx
Figure 2. Plasmid design pET29a(+) COMPy
