Team:TU Eindhoven/Microfluidics/Droplet Device

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                   <h2>Droplet Device</h2>
                   <h2>Droplet Device</h2>
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                   <p>To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used (<a href='#Fig1'>Figure 1</a>). Both channels will end in a so called flow-focusing cross junction, where the droplets will be formed. The fluids will first pass a filter to minimize blockage at the cross junction nozzle. The curved channels just before the cross junction are fluid resistors and will create a laminar flow. It is possible to control the droplet size and droplet formation speed, by alternating both the oil flow as the water flow.</p>
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                   <p>To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used (<nobr><a href='#Fig3'>Figure 3</a></nobr>). Both channels will end in a so called flow-focusing cross junction, where the droplets will be formed. The fluids will first pass a filter to minimize blockage at the cross junction nozzle. The curved channels just before the cross junction are fluid resistors and will create a laminar flow [1]. It is possible to control the droplet size and droplet formation frequency, by alternating the rates of the oil phase and the water phase.</p>
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<img id='Fig1' src="https://static.igem.org/mediawiki/2014/c/c9/TU_Eindhoven_Droplet_Device.png" class="image_wrapper image_fr" width="1085">
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<p>To download the AutoCAD design of this microfluidic droplet device, <a href='https://static.igem.org/mediawiki/2014/b/bf/TU_Eindhoven_Photomasks_TU_Eindhoven.zip' target="_blank">click here</a>. Design by Leroy Tan, Boris Arts & Rafiq Lubken.</p>
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<p style="font-size:18px;color:#CCCCCC;">Figure 1. A droplet device with 1 oil inlet (top), 1 water inlet (middle) and an outlet (bottom). Number 1 is the filter and number 2 is the flow focusing cross junction where the droplets are formed.</p>
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<img id='Fig1' src="https://static.igem.org/mediawiki/2014/4/45/TU_Eindhoven_Droplet_device.jpg" width="490" style="display: inline-block; border: 4px solid #00BAC6; padding: 4px; background: #222; margin-bottom: 10px;">
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<figcaption style="font-size:18px;color:#CCCCCC;">Figure 1. Microfluidic Droplet Device</figcaption>
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<img id='Fig2' src="https://static.igem.org/mediawiki/2014/9/9c/TU_Eindhoven_Wafer_droplet_device.jpg" width="490" style="display: inline-block; border: 4px solid #00BAC6; padding: 4px; background: #222; margin-bottom: 10px;">
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<figcaption style="font-size:18px;color:#CCCCCC;">Figure 2. Wafer for Droplet Device.</figcaption>
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<img id='Fig3' src="https://static.igem.org/mediawiki/2014/c/c9/TU_Eindhoven_Droplet_Device.png" class="image_wrapper image_fr" width="1085">
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<p style="font-size:18px;color:#CCCCCC;">Figure 3. A droplet device with 1 oil inlet (top), 1 water inlet (middle) and an outlet (bottom). Number 1 is the filter and number 2 is the flow focusing cross junction where the droplets are formed.</p>
<h4>Bibliography</h4>
<h4>Bibliography</h4>
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<p>Song H, Chen DL, Ismagilov RF. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. 2006;45:7336–7356
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[1] Mazutis, L., Gilbert, J., Ung. W.L., Weitz, D.A., Griffiths, A.D. & Heyman J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. <i>Nature</i>, 8(5), pp. 870-91.
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Mazutis, L., Gilbert, J., Ung. W.L., Weitz, D.A., Griffiths, A.D. & Heyman J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. Nature, 8(5), pp. 870-91.
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<br><br>
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Song H, Chen DL, Ismagilov RF. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. 2006;45:7336–7356
 
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Latest revision as of 00:19, 18 October 2014

iGEM Team TU Eindhoven 2014

iGEM Team TU Eindhoven 2014

Droplet Device

To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used (Figure 3). Both channels will end in a so called flow-focusing cross junction, where the droplets will be formed. The fluids will first pass a filter to minimize blockage at the cross junction nozzle. The curved channels just before the cross junction are fluid resistors and will create a laminar flow [1]. It is possible to control the droplet size and droplet formation frequency, by alternating the rates of the oil phase and the water phase.

To download the AutoCAD design of this microfluidic droplet device, click here. Design by Leroy Tan, Boris Arts & Rafiq Lubken.

Figure 1. Microfluidic Droplet Device
Figure 2. Wafer for Droplet Device.

Figure 3. A droplet device with 1 oil inlet (top), 1 water inlet (middle) and an outlet (bottom). Number 1 is the filter and number 2 is the flow focusing cross junction where the droplets are formed.

Bibliography

[1] Mazutis, L., Gilbert, J., Ung. W.L., Weitz, D.A., Griffiths, A.D. & Heyman J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. Nature, 8(5), pp. 870-91.

iGEM Team TU Eindhoven 2014

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