Team:Freiburg/Results

From 2014.igem.org

(Difference between revisions)
 
(19 intermediate revisions not shown)
Line 2: Line 2:
<html>
<html>
-
<div class="content">
+
<style>
 +
.header-right {
 +
  background: -moz-linear-gradient(0deg, #181617 0%, #181617 10%, rgba(24, 22, 23, 0) 30%), url(https://static.igem.org/mediawiki/2014/0/0f/Freiburg2014-09-24_NIH3t3_transduced_with_MuLV_EGFP_header_Thumbnail.jpg);
 +
  background: -webkit-linear-gradient(0deg, #181617 0%, #181617 10%, rgba(24, 22, 23, 0) 30%), url(https://static.igem.org/mediawiki/2014/0/0f/Freiburg2014-09-24_NIH3t3_transduced_with_MuLV_EGFP_header_Thumbnail.jpg);
 +
  background: linear-gradient(90deg, #181617 0%, #181617 10%, rgba(24, 22, 23, 0) 30%), url(https://static.igem.org/mediawiki/2014/0/0f/Freiburg2014-09-24_NIH3t3_transduced_with_MuLV_EGFP_header_Thumbnail.jpg);
 +
  background-size: 100% auto, 100% auto;
 +
  background-position: center center, center center;
 +
}
 +
</style>
</html>
</html>
-
{{:Team:Freiburg/Content/Project}}
+
 
 +
<html>
 +
<div class="wrapper-outer">
 +
  <div class="wrapper">
 +
    <div class="content-wrapper inner-page">
 +
      <div class="content">
 +
</html>
 +
{{:Team:Freiburg/Content/Results/Summary}}
<html>
<html>
</div>
</div>
 +
      <aside>
 +
        </html>{{:Team:Freiburg/Results/asidenav}}<html>
 +
      </aside>
 +
    </div>
 +
</html>
 +
{{:Team:Freiburg/Templates/html/foot.html}}
 +
<html>
 +
    </div>
 +
  </div>
 +
 +
</html>
 +
{{:Team:Freiburg/Templates/html/js.html}}
 +
<html>
 +
<script>
 +
    // hover over effects
 +
    (function(){
 +
      var left = $('#over-left'),
 +
        right = $('#over-right'),
 +
        links = $('#svg-schema-overview svg a');
 +
        console.dir(links);
 +
      function onHover(){
 +
        console.log("hovering");
 +
        // grab content
 +
        var content = $($(this).data('id'));
 +
 +
        // set content
 +
        var target = content.data('target');
 +
        console.dir(content);
 +
        console.dir(target);
 +
        if (target == '#over-right') right.html(content.html());
 +
        if (target == '#over-left') left.html(content.html());
 +
      };
 +
 +
      function onBlur(){
 +
        // clear content
 +
        right.html('');
 +
        left.html('');
 +
      }
 +
 +
      // add hover/blur handler
 +
      links.hover(onHover, onBlur);
 +
    })();
 +
  </script>
 +
<script>
 +
// set title
 +
$('head title').text('Results | Team Freiburg | iGEM2014');
 +
</script>
</html>
</html>

Latest revision as of 21:56, 17 October 2014

The AcCELLerator

The AcCELLerator

Summary



We, the iGEM Team Freiburg 2014, have the goal to combine the capability of viral vectors for stable gene transfer with the spatio-temporal resolution of optogenetics for gene delivery into mammalian cells. We utilized the Murine Leukemia Virus (MuLV) that specifically uses the murine mCAT-1 receptor to infect target cells. By using a blue light inducible expression system, we brought the receptor to the plasma membrane of illuminated cells to enable targeted gene delivery by the virus.

Our project was quite a large success! We:

  • produced MuLV viruses containing different reporter proteins,
  • optimized MuLV production and transduction protocols to reach close to 100% efficiency,
  • created stable mammalian cell lines using the MuLV virus,
  • generated patterns of reporter proteins in cell cultures by light exposure,
  • demonstrated that the virus exclusively infects cells expressing the mCAT-1 receptor,
  • infected cells with the MuLV virus that expressed the mCAT-1 receptor after light induction.

We provide the virus as a tool for the generation of stable mammalian cell lines under biosafety level 1 regulations. Our viral vector, which we propose as a new iGEM RFC, enables the user to introduce any gene of interest stably into mammalian cell lines. Therefore we provide for the iGEM community a fast, easy to handle and safe way of generating stable cell lines.

During the course of iGEM, we learned many new techniques:

  • mammalian cell culture,
  • fluorescence activated cell sorting,
  • virus production under biosafety level 1 and biosafety level 2 conditions,
  • many different cloning techniques,
  • widefield and confocal microscopy,
  • creating a website,

and, last but not least, we had a lot of fun and learned how to work together as a team!

Retrieved from "http://2014.igem.org/Team:Freiburg/Results"