Team:TU Eindhoven/Microfluidics/Cell Encapsulation Device

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                   <h2>Droplet Device</h2>
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                   <h2>Cell Encapsulation Device</h2>
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                   <p>To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used (<a href='#Fig1'>Figure 1</a>). Both channels will end in a so called flow-focusing cross junction, where the droplets will be formed. The fluids will first pass a filter to minimize blockage at the cross junction nozzle. The curved channels just before the cross junction are fluid resistors and will create a laminar flow. It is possible to control the droplet size and droplet formation speed, by alternating both the oil flow as the water flow.</p>
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                   <p>The cell encapsulation device (<a href='#Fig3'>Figure 3</a> and <a href='#Fig4'>Figure 4</a>) is based on the droplet device shown earlier. However, this time there are two continuous phases (co-flow) that come together at the first flow-focusing cross junction. One containing cells and the other containing a PEG solution. At this point the two continuous phases will create a laminar flow next to each other. At the second flow-focusing cross junction, encapsulation of cells will take place according to the same principle as in the droplet device. Once droplets are formed, they pass through a bumpy mixer [1]. This will ensure that the content is homogeneously distributed in the droplet.  
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Next the droplets will pass through a droplet chamber. This chamber is designed to hold up to a thousand droplets evenly distributed. The twelve pillars inside the chamber prevent the chamber from collapsing. After the flows are stabilized and droplets are formed, they can be trapped in the chamber. In the end the inlets and outlet can be cauterized to prevent the droplets from escaping. Now the droplets can simply be analyzed with a microscope.
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<p>For an AutoCAD design of this microfluidic droplet device, download <a href='#'>here</a>. Design by Leroy Tan, Boris Arts & Rafiq Lubken.</p>
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<p>To download the AutoCAD design of this microfluidic droplet device, <a href='https://static.igem.org/mediawiki/2014/b/bf/TU_Eindhoven_Photomasks_TU_Eindhoven.zip' target="_blank">click here</a>. Design by Leroy Tan, Boris Arts & Rafiq Lubken.</p>
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<img id='Fig1' src="https://static.igem.org/mediawiki/2014/c/c9/TU_Eindhoven_Droplet_Device.png" class="image_wrapper image_fr" width="1085">
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<p style="font-size:18px;color:#CCCCCC;">Figure 1. A droplet device with 1 oil inlet (top), 1 water inlet (middle) and an outlet (bottom). Number 1 is the filter and number 2 is the flow focusing cross junction where the droplets are formed.</p>
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<img id='Fig1' src="https://static.igem.org/mediawiki/2014/9/92/TU_Eindhoven_Cell_encapsulation_device.jpg" width="490" style="display: inline-block; border: 4px solid #00BAC6; padding: 4px; background: #222; margin-bottom: 10px;">
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<figcaption style="font-size:18px;color:#CCCCCC;">Figure 1. Microfluidic Cell Encapsulation Device</figcaption>
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<img id='Fig2' src="https://static.igem.org/mediawiki/2014/3/34/TU_Eindhoven_Wafer_cell_encapsulation_device.jpg" width="490" style="display: inline-block; border: 4px solid #00BAC6; padding: 4px; background: #222; margin-bottom: 10px;">
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<figcaption style="font-size:18px;color:#CCCCCC;">Figure 2. Wafer for Cell Encapsulation Device.</figcaption>
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<img id='Fig3' src="https://static.igem.org/mediawiki/2014/1/1e/TU_Eindhoven_Cell_Encapsulation_Device.jpg" class="image_wrapper image_fr" width="1085">
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<p style="font-size:18px;color:#CCCCCC;">Figure 3. From top to bottom: oil inlet, continuous phase 2 inlet (containing PEG-DBCO), continuous phase 1 inlet (containing <i>E. coli</i>) and outlet.</p>
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<img id='Fig4' src="https://static.igem.org/mediawiki/2014/a/a5/TU_Eindhoven_Cell_Encapsulation_Rendered.jpg" class="image_wrapper image_fr" width="1085">
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<p style="font-size:18px;color:#CCCCCC;">Figure 4. The first flow-focusing cross junction is at the point where the orange channels and the yellow channel are coming together. The second flow-focusing cross junction is at the point where the orange and red channels are coming together.</p>
<h4>Bibliography</h4>
<h4>Bibliography</h4>
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<p>Song H, Chen DL, Ismagilov RF. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. 2006;45:7336–7356
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[1] Song H, Chen DL, Ismagilov RF. (2006). Reactions in droplets in microfluidic channels. <i>Angew Chem Int Ed Engl</i>;45:7336–7356.
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Mazutis, L., Gilbert, J., Ung. W.L., Weitz, D.A., Griffiths, A.D. & Heyman J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. Nature, 8(5), pp. 870-91.
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Song H, Chen DL, Ismagilov RF. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. 2006;45:7336–7356
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Latest revision as of 00:29, 18 October 2014

iGEM Team TU Eindhoven 2014

iGEM Team TU Eindhoven 2014

Cell Encapsulation Device

The cell encapsulation device (Figure 3 and Figure 4) is based on the droplet device shown earlier. However, this time there are two continuous phases (co-flow) that come together at the first flow-focusing cross junction. One containing cells and the other containing a PEG solution. At this point the two continuous phases will create a laminar flow next to each other. At the second flow-focusing cross junction, encapsulation of cells will take place according to the same principle as in the droplet device. Once droplets are formed, they pass through a bumpy mixer [1]. This will ensure that the content is homogeneously distributed in the droplet.

Next the droplets will pass through a droplet chamber. This chamber is designed to hold up to a thousand droplets evenly distributed. The twelve pillars inside the chamber prevent the chamber from collapsing. After the flows are stabilized and droplets are formed, they can be trapped in the chamber. In the end the inlets and outlet can be cauterized to prevent the droplets from escaping. Now the droplets can simply be analyzed with a microscope.

To download the AutoCAD design of this microfluidic droplet device, click here. Design by Leroy Tan, Boris Arts & Rafiq Lubken.

Figure 1. Microfluidic Cell Encapsulation Device
Figure 2. Wafer for Cell Encapsulation Device.

Figure 3. From top to bottom: oil inlet, continuous phase 2 inlet (containing PEG-DBCO), continuous phase 1 inlet (containing E. coli) and outlet.

Figure 4. The first flow-focusing cross junction is at the point where the orange channels and the yellow channel are coming together. The second flow-focusing cross junction is at the point where the orange and red channels are coming together.

Bibliography

[1] Song H, Chen DL, Ismagilov RF. (2006). Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl;45:7336–7356.

iGEM Team TU Eindhoven 2014

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