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Team:TU Eindhoven/Protocols
From 2014.igem.org
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<td><a target="_blank" href="https://static.igem.org/mediawiki/2014/e/eb/TU_Eindhoven_Antibody_labelling.pdf">Antibody Labelling</a></td> | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/e/eb/TU_Eindhoven_Antibody_labelling.pdf">Antibody Labelling</a></td> | ||
| - | <td><a target="_blank" | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/9/94/TU_Eindhoven_Protocol_Double_Transformation.pdf">Double Transformation</a> </td> |
| - | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/ | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/2/2b/TU_Eindhoven_Protocol_Plasmid_purification.pdf">Plasmid Purification</a> </td> |
</tr> | </tr> | ||
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<td><a target="_blank" href="https://static.igem.org/mediawiki/2014/1/16/TU_Eindhoven_Protocol_FACS_%28Antibody_Titration%29.pdf">Antibody Titration with FACS</a></td> | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/1/16/TU_Eindhoven_Protocol_FACS_%28Antibody_Titration%29.pdf">Antibody Titration with FACS</a></td> | ||
| - | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/ | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/d/de/TU_Eindhoven_FACS_%28DBCO-PEG_10_kDa%29.pdf">FACS DBCO-PEG(10kDa)</a> </td> |
| - | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/ | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/3/35/TU_Eindhoven_Protocol_Preparative_steps.pdf">Preparative Steps</a> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href="https://static.igem.org/mediawiki/2014/8/80/TU_Eindhoven_Protocol_bacteria_culturing_for_microfluidics.pdf" target="_blank">Bacteria Cultering for Microfluidics</a></td> | <td><a href="https://static.igem.org/mediawiki/2014/8/80/TU_Eindhoven_Protocol_bacteria_culturing_for_microfluidics.pdf" target="_blank">Bacteria Cultering for Microfluidics</a></td> | ||
| - | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/ | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/0/07/TU_Eindhoven_Protocol_FACS_%28DBCO-56-TAMRA%29l.pdf">FACS for sorting with DBCO-TAMRA</a> </td> |
| - | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/ | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6f/TU_Eindhoven_Protocol_Protein_expression.pdf">Protein Expression</a> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/0/00/TU_Eindhoven_Casting_and_running_PAGE_gel.pdf">Casting and Running 15% PAGE Gel</a> </td> | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/0/00/TU_Eindhoven_Casting_and_running_PAGE_gel.pdf">Casting and Running 15% PAGE Gel</a> </td> | ||
| - | <td> <a target="_blank" | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/c/cc/TU_Eindhoven_Protocol_Labelling_antibodies_with_56-TAMRA-NHS.pdf">Fluorescent labelling antibodies</a> </td> |
| - | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/ | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/8/8f/TU_Eindhoven_Protocol_Rolling_Circle_Amplification_on_cell_membrane.pdf">Rolling Circle Amplification on membrane</a> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/ | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6c/TU_Eindhoven_Protocol_Cell_viability.pdf">Cell Viability Assay</a> </td> |
| - | + | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/1/1e/TU_Eindhoven_Protocol_Overhang_PCR.pdf">Overhang PCR</a><a target="_blank" href="https://static.igem.org/mediawiki/2014/9/94/TU_Eindhoven_Protocol_Double_Transformation.pdf"></a></td> | |
| - | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/ | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/7/74/TU_Eindhoven_Protocol_Site_Directed_Mutagenesis.pdf">Site Directed Mutagenesis</a> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/ | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/3/3d/TU_Eindhoven_Protocol_Colony_PCR.pdf">Colony PCR</a> </td> |
| - | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/ | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/9/90/TU_Eindhoven_Protocol_PCR_Purification_of_Insert_Fragment.pdf">PCR Purification of DNA Fragments</a> </td> |
| - | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/ | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/3/35/TU_Eindhoven_Protocol_Insert_%2B_Vector_Ligation.pdf">Vector Ligation</a> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/ | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/5/50/TU_Eindhoven_Creating_circular_RCA_template.pdf">Creating Circular RCA Template</a> </td> |
| - | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/ | + | <td> <a target="_blank" href="https://static.igem.org/mediawiki/2014/7/7d/TU_Eindhoven_Protocol_Plasmid_and_gene_digestion.pdf">Plasmid Gene Digestion</a> </td> |
| - | <td> | + | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/8/8a/TU_Eindhoven_Protocol_Transformation.pdf">Vector Transformation</a><a target="_blank" href="https://static.igem.org/mediawiki/2014/6/6c/TU_Eindhoven_Protocol_Cell_viability.pdf"></a></td> |
</tr> | </tr> | ||
</table> | </table> | ||
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| - | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/2/2f/TU_Eindhoven_Labelling_amine-modified_DNA_with_DBCO-PEG4-NHS_ester.pdf">Labelling Amine-Modified DNA with DBCO- | + | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/2/2f/TU_Eindhoven_Labelling_amine-modified_DNA_with_DBCO-PEG4-NHS_ester.pdf">Labelling Amine-Modified DNA with DBCO-PEG<sub>4</sub>-NHS Ester</a></td> |
</tr> | </tr> | ||
</table> | </table> | ||
| - | <h3>Microfluidics Protocols</h3> | + | <h3 id='Micro'>Microfluidics Protocols</h3> |
<p><em>The following protocols are used in the Microfabrication lab for the production and running of microfluidic devices.</em></p> | <p><em>The following protocols are used in the Microfabrication lab for the production and running of microfluidic devices.</em></p> | ||
<table border="0" style="width:1085px;background:none;"> | <table border="0" style="width:1085px;background:none;"> | ||
<tr> | <tr> | ||
| - | + | <td><a href="https://static.igem.org/mediawiki/2014/2/2e/TU_Eindhoven_Protocol_Microfluidic_Device_Testing_%28Droplets%29.pdf" target="_blank">Droplet Device Testing</a></td> | |
<td><a target="_blank" href="https://static.igem.org/mediawiki/2014/d/d5/TU_Eindhoven_Protocol_oil_and_continuous_phase.pdf">Oil and Water Phase (Polyacrylamide Beads)</a></td> | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/d/d5/TU_Eindhoven_Protocol_oil_and_continuous_phase.pdf">Oil and Water Phase (Polyacrylamide Beads)</a></td> | ||
| - | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | + | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/0/09/TU_Eindhoven_Protocol_droplet_separation.pdf">Droplet Separation</a></td> | |
| - | <td><a href="https://static.igem.org/mediawiki/2014/9/ | + | <td><a href="https://static.igem.org/mediawiki/2014/9/9f/TU_Eindhoven_Protocol_Photolithography.pdf" target="_blank">Photolithography</a></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td><a href="https://static.igem.org/mediawiki/2014/ | + | <td><a href="https://static.igem.org/mediawiki/2014/1/1e/TU_Eindhoven_Protocol_oil_and_water_phase_bead_and_bacterial_cell_encapsulation.pdf" target="_blank">Oil and Water Phase (Encapsulation)</a></td> |
| - | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/ | + | <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/8/89/TU_Eindhoven_Protocol_Soft_Lithography.pdf">Soft Lithography</a></td> |
</tr> | </tr> | ||
Latest revision as of 00:06, 18 October 2014
Protocols
For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.
Genetic Engineering Protocols
The following protocols are used in the Biolab during the modification of bacteria.
Chemistry Protocols
The following protocols are used in the chemical synthesis processes.
| SPAAC reaction monitoring with UV-Visible Spectroscopy |
| Labelling Amine-Modified DNA with DBCO-PEG4-NHS Ester |
Microfluidics Protocols
The following protocols are used in the Microfabrication lab for the production and running of microfluidic devices.
| Droplet Device Testing | Oil and Water Phase (Polyacrylamide Beads) |
| Droplet Separation | Photolithography |
| Oil and Water Phase (Encapsulation) | Soft Lithography |
