Team:Freiburg/Results

From 2014.igem.org

(Difference between revisions)
 
(8 intermediate revisions not shown)
Line 4: Line 4:
<style>
<style>
.header-right {
.header-right {
-
   background: -moz-linear-gradient(0deg, #181617 0%, #181617 10%, rgba(24, 22, 23, 0) 30%), url(https://static.igem.org/mediawiki/2014/f/fb/2014_Freiburg_Abstract.png);
+
   background: -moz-linear-gradient(0deg, #181617 0%, #181617 10%, rgba(24, 22, 23, 0) 30%), url(https://static.igem.org/mediawiki/2014/0/0f/Freiburg2014-09-24_NIH3t3_transduced_with_MuLV_EGFP_header_Thumbnail.jpg);
-
   background: -webkit-linear-gradient(0deg, #181617 0%, #181617 10%, rgba(24, 22, 23, 0) 30%), url(https://static.igem.org/mediawiki/2014/f/fb/2014_Freiburg_Abstract.png);
+
   background: -webkit-linear-gradient(0deg, #181617 0%, #181617 10%, rgba(24, 22, 23, 0) 30%), url(https://static.igem.org/mediawiki/2014/0/0f/Freiburg2014-09-24_NIH3t3_transduced_with_MuLV_EGFP_header_Thumbnail.jpg);
-
   background: linear-gradient(90deg, #181617 0%, #181617 10%, rgba(24, 22, 23, 0) 30%), url(https://static.igem.org/mediawiki/2014/f/fb/2014_Freiburg_Abstract.png);
+
   background: linear-gradient(90deg, #181617 0%, #181617 10%, rgba(24, 22, 23, 0) 30%), url(https://static.igem.org/mediawiki/2014/0/0f/Freiburg2014-09-24_NIH3t3_transduced_with_MuLV_EGFP_header_Thumbnail.jpg);
   background-size: 100% auto, 100% auto;
   background-size: 100% auto, 100% auto;
   background-position: center center, center center;
   background-position: center center, center center;
-
}
 
-
.wrapper-outer {
 
-
background-color: #181617;
 
}
}
</style>
</style>
Line 26: Line 23:
</div>
</div>
       <aside>
       <aside>
-
         <!-- ul>li*4>(a>lorem2)+ul>li*2>a>lorem2 -->
+
         </html>{{:Team:Freiburg/Results/asidenav}}<html>
-
 
+
-
        <div id="content-nav">
+
-
          <ul class="nav">
+
-
            <li class="active">
+
-
              <a href="#a-section1">Section 1</a>
+
-
              <ul>
+
-
                <li><a href="#a-section1-1">Section 1.1</a></li>
+
-
                <li><a href="#a-lists">Lists</a></li>
+
-
                <li><a href="#a-lists-unordered">Unordered</a></li>
+
-
                <li><a href="#a-lists-ordered">Ordered</a></li>
+
-
              </ul>
+
-
            </li>
+
-
            <li>
+
-
              <a href="#a-accordion">Accordion</a>
+
-
            </li>
+
-
            <li>
+
-
              <a href="#a-page-links">Links to pages</a>
+
-
            </li>
+
-
            <li>
+
-
              <a href="#a-abstract">Project Abstract</a>
+
-
            </li>
+
-
            <li>
+
-
              <a href="#a-team">The Team</a>
+
-
              <ul>
+
-
                <li><a href="#a-team-members">Team members</a></li>
+
-
                <li><a href="#a-team-instructors">Instructors</a></li>
+
-
                <li><a href="#a-team-advisor">Advisor</a></li>
+
-
              </ul>
+
-
            </li>
+
-
          </ul>
+
-
        </div>
+
       </aside>
       </aside>
     </div>
     </div>
Line 69: Line 35:
{{:Team:Freiburg/Templates/html/js.html}}
{{:Team:Freiburg/Templates/html/js.html}}
<html>
<html>
 +
<script>
 +
    // hover over effects
 +
    (function(){
 +
      var left = $('#over-left'),
 +
        right = $('#over-right'),
 +
        links = $('#svg-schema-overview svg a');
 +
        console.dir(links);
 +
      function onHover(){
 +
        console.log("hovering");
 +
        // grab content
 +
        var content = $($(this).data('id'));
 +
 +
        // set content
 +
        var target = content.data('target');
 +
        console.dir(content);
 +
        console.dir(target);
 +
        if (target == '#over-right') right.html(content.html());
 +
        if (target == '#over-left') left.html(content.html());
 +
      };
 +
 +
      function onBlur(){
 +
        // clear content
 +
        right.html('');
 +
        left.html('');
 +
      }
 +
 +
      // add hover/blur handler
 +
      links.hover(onHover, onBlur);
 +
    })();
 +
  </script>
<script>
<script>
// set title  
// set title  

Latest revision as of 21:56, 17 October 2014

The AcCELLerator

The AcCELLerator

Summary



We, the iGEM Team Freiburg 2014, have the goal to combine the capability of viral vectors for stable gene transfer with the spatio-temporal resolution of optogenetics for gene delivery into mammalian cells. We utilized the Murine Leukemia Virus (MuLV) that specifically uses the murine mCAT-1 receptor to infect target cells. By using a blue light inducible expression system, we brought the receptor to the plasma membrane of illuminated cells to enable targeted gene delivery by the virus.

Our project was quite a large success! We:

  • produced MuLV viruses containing different reporter proteins,
  • optimized MuLV production and transduction protocols to reach close to 100% efficiency,
  • created stable mammalian cell lines using the MuLV virus,
  • generated patterns of reporter proteins in cell cultures by light exposure,
  • demonstrated that the virus exclusively infects cells expressing the mCAT-1 receptor,
  • infected cells with the MuLV virus that expressed the mCAT-1 receptor after light induction.

We provide the virus as a tool for the generation of stable mammalian cell lines under biosafety level 1 regulations. Our viral vector, which we propose as a new iGEM RFC, enables the user to introduce any gene of interest stably into mammalian cell lines. Therefore we provide for the iGEM community a fast, easy to handle and safe way of generating stable cell lines.

During the course of iGEM, we learned many new techniques:

  • mammalian cell culture,
  • fluorescence activated cell sorting,
  • virus production under biosafety level 1 and biosafety level 2 conditions,
  • many different cloning techniques,
  • widefield and confocal microscopy,
  • creating a website,

and, last but not least, we had a lot of fun and learned how to work together as a team!

Retrieved from "http://2014.igem.org/Team:Freiburg/Results"