Team:TU Eindhoven/Protocols

Protocols

For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.

Genetic Engineering Protocols

The following protocols are used in the Biolab during the modification of bacteria.

Antibody Labeling	Overhang PCR	Protein Expression Curve
Antibody Titration with FACS	PCR Purification of DNA Fragments	Site Directed Mutagenesis
Antifouling	Plasmid Amplification	Vector Ligation
Casting and Running 15% PAGE Gel	Plasmid Gene Digestion	Vector Transformation
Creating Circular RCA Template	Plasmid Purification
FACS for sorting with DBCO-PEG(10kDa)	Preparative Steps
FACS for sorting with DBCO-TAMRA	Protein Expression

Chemistry Protocols

The following protocols are used in the chemical synthesis processes.

SPAAC reaction monitoring with UV-Visible Spectroscopy

Labeling Amine-Modified DNA with DBCO-PEG4-NHS Ester

Microfluidics Protocols

The following protocols are used in the Microfabrication lab for the production and running of microfluidic devices.

Bacteria Cultering for Microfluidics	Oil and Water Phase (Polyacrylamide Beads)
Droplet Separation	Photolithography
Droplet Device Testing	Soft Lithography
Oil and Water Phase (Bacterial Encapsulation)