Team:TU Eindhoven/Protocols

From 2014.igem.org

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                   <h3>Chemistry Protocols</h3>
                   <h3>Chemistry Protocols</h3>
<p><em>The following protocols are used in the chemical synthesis processes.</em></p>
<p><em>The following protocols are used in the chemical synthesis processes.</em></p>
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    <td><a href="https://static.igem.org/mediawiki/2014/3/3d/Protocol_-_Monitoring_SPAAC_with_UVVIS.pdf" target="_blank">SPAAC reaction monitoring with UV-Visible Spectroscopy</a></td>
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    <td><a target="_blank" href="https://static.igem.org/mediawiki/2014/2/2f/TU_Eindhoven_Labelling_amine-modified_DNA_with_DBCO-PEG4-NHS_ester.pdf">Labeling Amine-Modified DNA with DBCO-PEG4-NHS Ester</a></td>
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<a href="
<a href="
https://static.igem.org/mediawiki/2014/3/3d/Protocol_-_Monitoring_SPAAC_with_UVVIS.pdf">SPAAC reaction monitoring with UV-Visible Spectroscopy</a>
https://static.igem.org/mediawiki/2014/3/3d/Protocol_-_Monitoring_SPAAC_with_UVVIS.pdf">SPAAC reaction monitoring with UV-Visible Spectroscopy</a>

Revision as of 12:35, 14 October 2014

iGEM Team TU Eindhoven 2014

iGEM Team TU Eindhoven 2014

Protocols

For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.

Genetic Engineering Protocols

The following protocols are used in the Biolab during the modification of bacteria.

Antibody Labeling Overhang PCR Protein Expression Curve
Antibody Titration with FACS PCR Purification of DNA Fragments Site Directed Mutagenesis
Antifouling Plasmid Amplification Vector Ligation
Casting and Running 15% PAGE Gel Plasmid Gene Digestion Vector Transformation
Creating Circular RCA Template Plasmid Purification
FACS for sorting with DBCO-PEG(10kDa) Preparative Steps
FACS for sorting with DBCO-TAMRA Protein Expression

Chemistry Protocols

The following protocols are used in the chemical synthesis processes.

SPAAC reaction monitoring with UV-Visible Spectroscopy Labeling Amine-Modified DNA with DBCO-PEG4-NHS Ester
SPAAC reaction monitoring with UV-Visible Spectroscopy

Microfluidics Protocols

The following protocols are used in the Microfabrication lab for the production and running of microfluidic devices.

iGEM Team TU Eindhoven 2014

Retrieved from "http://2014.igem.org/Team:TU_Eindhoven/Protocols"