Team:TU Eindhoven/Protocols

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Revision as of 07:47, 14 October 2014

iGEM Team TU Eindhoven 2014

iGEM Team TU Eindhoven 2014

Protocols

For this year's iGEM competition numerous protocols were devoloped to guide our experiments and keep our documentation neat and tidy. Because these protocols can also be useful to other projects, we decided to publish them on our wiki. You can find information and download links on this page down below.

Genetic Engineering Protocols

The following protocols are used in the Biolab during the modification of bacteria.

Antibody Titration with FACS Overhang PCR Plasmid Gene Digestion
FACS for sorting with DBCO-TAMRA FACS for sorting with DBCO-PEG(10kDa) Vector Ligation
Plasmid Amplification Plasmid Purification Preparative Steps
Vector Transformation Site Directed Mutagenesis Protein Expression
Protein Expression PCR purification of DNA fragments Protein Expression Curve
Antibody Labeling Antifouling

Chemistry Protocols

The following protocols are used in ...

SPAAC reaction monitoring with UV-Visible Spectroscopy

Microfluidics Protocols

The following protocols are used in the Microfabrication lab for the production and running of microfluidic devices.

iGEM Team TU Eindhoven 2014

Retrieved from "http://2014.igem.org/Team:TU_Eindhoven/Protocols"