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Team:TU Eindhoven/Project/Characterization/Click Coli
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<div class="col_700_2 float_r"> | <div class="col_700_2 float_r"> | ||
<figure style="float:right;margin-right:0;"> | <figure style="float:right;margin-right:0;"> | ||
| - | <img id='Fig1' src="https://static.igem.org/mediawiki/2014/ | + | <img id='Fig1' src="https://static.igem.org/mediawiki/2014/0/06/TU_Eindhoven_Chemical_Structure_of_TAMRA-DBCO.png" width="500" style="display: inline-block; border: 4px solid #00BAC6; padding: 4px; background: #222; margin-bottom: 10px;"> |
| - | <figcaption style="font-size:18px;color:#CCCCCC;"> | + | <figcaption style="font-size:18px;color:#CCCCCC;">Figure 1. Chemical structure of DBCO-PEG<sub>4</sub>-5/6-TAMRA.</figcaption> |
</figure> | </figure> | ||
<h2>Click Coli</h2> | <h2>Click Coli</h2> | ||
| - | + | <p>Now that the plasmid design has been verified using the sequencing results and the SPAAC reaction has been verified using UV-VIS and LCMS, it is time to check whether the plasmids result in the wanted protein expression. To verify the expression of Clickable Outer Membrane Protein X (COMPx) and Y (COMPy), fluorescent labelled dibenzocyclooctyne (DBCO) groups, DBCO-PEG4-5/6-TAMRA (Figure 1), have been reacted with the incorporated p-azido-L-phenylalanine (pAzF).</p> | |
<h4>Bibliography</h4> | <h4>Bibliography</h4> | ||
Revision as of 19:03, 13 October 2014
Click Coli
Now that the plasmid design has been verified using the sequencing results and the SPAAC reaction has been verified using UV-VIS and LCMS, it is time to check whether the plasmids result in the wanted protein expression. To verify the expression of Clickable Outer Membrane Protein X (COMPx) and Y (COMPy), fluorescent labelled dibenzocyclooctyne (DBCO) groups, DBCO-PEG4-5/6-TAMRA (Figure 1), have been reacted with the incorporated p-azido-L-phenylalanine (pAzF).
