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Team:TU Eindhoven/Modeling/Bacterial Cell Counter
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<img id='Fig1' src="https://static.igem.org/mediawiki/2014/b/be/TU_Eindhoven_Modeling4.png" width="500" style="display: inline-block; border: 4px solid #00BAC6; padding: 4px; background: #222; margin-bottom: 10px;"> | <img id='Fig1' src="https://static.igem.org/mediawiki/2014/b/be/TU_Eindhoven_Modeling4.png" width="500" style="display: inline-block; border: 4px solid #00BAC6; padding: 4px; background: #222; margin-bottom: 10px;"> | ||
<figcaption style="font-size:18px;color:#CCCCCC;">Figuur 1. Sample image used to show how the program works.</figcaption> | <figcaption style="font-size:18px;color:#CCCCCC;">Figuur 1. Sample image used to show how the program works.</figcaption> | ||
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| + | <h2>Bacterial Cell Counter</h2> | ||
| + | <p>When photos of the microfluidics results are taken, the droplets and the cells in the droplets have to be counted. In order to have reliable results this has to be done on a large scale. To make it possible to analyses large numbers of photos a computer program has been made to do the counting. To give a better visualization of the process, a step by step analysis of an image is given below.</p> | ||
| - | + | <p>The program has the following steps: find and count the droplets, then find and count the cells in the droplets and finally create a histogram of the results (number of droplets with 0 cells, with 1 cell, with 2 et cetera). By adding up the histograms of multiple images you get a view of how the cells are divided over the droplets.</p> | |
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<img id='Fig2' src="https://static.igem.org/mediawiki/2014/0/09/TU_Eindhoven_Modeling2.jpg" class="image_wrapper image_fr" width="1085"> | <img id='Fig2' src="https://static.igem.org/mediawiki/2014/0/09/TU_Eindhoven_Modeling2.jpg" class="image_wrapper image_fr" width="1085"> | ||
Revision as of 10:35, 11 October 2014
Bacterial Cell Counter
When photos of the microfluidics results are taken, the droplets and the cells in the droplets have to be counted. In order to have reliable results this has to be done on a large scale. To make it possible to analyses large numbers of photos a computer program has been made to do the counting. To give a better visualization of the process, a step by step analysis of an image is given below.
The program has the following steps: find and count the droplets, then find and count the cells in the droplets and finally create a histogram of the results (number of droplets with 0 cells, with 1 cell, with 2 et cetera). By adding up the histograms of multiple images you get a view of how the cells are divided over the droplets.
Figure 2. Ratio between one cell/ zero cells or more cells
Based on these results a lambda of 0.5 was chosen for the microfluidic device. The Poisson distribution for different number of cells becomes then as follows.
Figure 3.
