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Team:TU Eindhoven/Design/Membrane Anchor Design
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Revision as of 20:21, 9 October 2014
Membrane Anchor Design
In short, both membrane proteins had a TAG codon introduced for the Non Natural Amino Acid and a HA-tag for detection methods. For INPNC the TAG codon and the HA-tag were introduced at the C terminus of the protein (Figure 1). The CPX proteins already contained a peptide library part at the N-terminus so the TAG codon was introduced in that part. This has been done by using Site Directed Mutagenesis. The HA-tag was introduced at the C-terminus (Figure 2). After modification the proteins were renamed to Clickable Outer Membrane Protein x (COMPx), originally CPX, and COMPy, originally INPNC.
Figure 1. Gene design COMPy (INPNC).
Figure 2. Gene design COMPy (CPX)
