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Team:TU Eindhoven/Microfluidics/Droplet Device
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<p>For an AutoCAD design of this microfluidic droplet device, download <a href='#'>here</a>. Design by Leroy Tan, Boris Arts & Rafiq Lubken.</p> | <p>For an AutoCAD design of this microfluidic droplet device, download <a href='#'>here</a>. Design by Leroy Tan, Boris Arts & Rafiq Lubken.</p> | ||
| - | <img id='Fig1' src="https://static.igem.org/mediawiki/2014/ | + | <img id='Fig1' src="https://static.igem.org/mediawiki/2014/a/a5/TU_Eindhoven_Cell_Encapsulation_Rendered.jpg" width="1085"> |
| - | <p style="font-size:18px;color:#CCCCCC;">Figure | + | <p style="font-size:18px;color:#CCCCCC;">Figure 2. The first flow-focusing cross junction is at the point where the orange channel and the yellow channel is coming together. The second flow-focusing cross junction is at the point where the orange and red channels are coming together.</p> |
<h4>Bibliography</h4> | <h4>Bibliography</h4> | ||
Revision as of 11:25, 9 October 2014
Droplet Device
To answer the questions mentioned at the introduction, a droplet device with a single oil inlet and a single water inlet is used (Figure 1). Both channels will end in a so called flow-focusing cross junction, where the droplets will be formed. The fluids will first pass a filter to minimize blockage at the cross junction nozzle. The curved channels just before the cross junction are fluid resistors and will create a laminar flow. It is possible to control the droplet size and droplet formation speed, by alternating both the oil flow as the water flow.
For an AutoCAD design of this microfluidic droplet device, download here. Design by Leroy Tan, Boris Arts & Rafiq Lubken.
Figure 2. The first flow-focusing cross junction is at the point where the orange channel and the yellow channel is coming together. The second flow-focusing cross junction is at the point where the orange and red channels are coming together.
Bibliography
Song H, Chen DL, Ismagilov RF. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. 2006;45:7336–7356
Mazutis, L., Gilbert, J., Ung. W.L., Weitz, D.A., Griffiths, A.D. & Heyman J.A. (2013). Single-cell analysis and sorting using droplet-based microfluidics. Nature, 8(5), pp. 870-91.
Song H, Chen DL, Ismagilov RF. Reactions in droplets in microfluidic channels. Angew Chem Int Ed Engl. 2006;45:7336–7356
