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Team:TU Eindhoven/Safety/Our Lab
From 2014.igem.org
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<h2>Our Lab</h2> | <h2>Our Lab</h2> | ||
| - | <p>The laboratory of Chemical Biology (see <a href='#Fig1'>Figure 1</a> for a plan) is located in the building Helix on the TU Eindhoven campus. This laboratory is part of the department of Biomedical Engineering. The laboratory of Chemical Biology | + | <p>The laboratory of Chemical Biology (see <a href='#Fig1'>Figure 1</a> for a plan) is located in the building Helix on the TU Eindhoven campus. This laboratory is part of the department of Biomedical Engineering. The laboratory of Chemical Biology consists of catagories two laboratories. The first one is an ML-I laboratory with biosafety level 1 (WHO BSL 1). Most of the work at the ML-I lab involves recombinant expression of proteins in <i>E. coli</i> or yeast expression systems and the application of bacteriophages for phage display. The second one is an ML-II laboratory (cell lab) with biosafety level 2 (WHO BSL 2). Work in the ML-II lab typically involves the use of immortalized cell lines, but it can also involve tissues or primary cells from animals or humans.</p> |
<img id='Fig1' src="https://static.igem.org/mediawiki/2014/a/ad/TU_Eindhoven_Laboratory.png" class="image_wrapper image_fr" width="1085"> | <img id='Fig1' src="https://static.igem.org/mediawiki/2014/a/ad/TU_Eindhoven_Laboratory.png" class="image_wrapper image_fr" width="1085"> | ||
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<h3>Safety Equipment</h3> | <h3>Safety Equipment</h3> | ||
| - | <p>The basic protection equipment that we use in the wet lab are lab coats, safety goggles and gloves. Lab coats and safety glasses are | + | <p>The basic protection equipment that we use in the wet lab are lab coats, safety goggles and gloves. Lab coats and safety glasses are alway sused and everywhere in the lab (ML-I and ML-II). Gloves (mostly latex) are used when handling chemicals and for working DNase free. In the cell lab (ML-II) they are also used for working with biological materials in the biosafety cabinets. Nitrile gloves are used for handling chemicals that pass through latex or for persons allergic to latex. Cryo-gloves and heat-resistant gloves are used for handling cold and hot materials respectively. Also when working with hazardous or toxic fumes used the fume hood.</p> |
<img id='Fig2' src="https://static.igem.org/mediawiki/2014/e/ed/TU_Eindhoven_Safety_Gloves.JPG" class="image_wrapper image_fr" width="1085"> | <img id='Fig2' src="https://static.igem.org/mediawiki/2014/e/ed/TU_Eindhoven_Safety_Gloves.JPG" class="image_wrapper image_fr" width="1085"> | ||
Revision as of 20:57, 17 October 2014
Our Lab
The laboratory of Chemical Biology (see Figure 1 for a plan) is located in the building Helix on the TU Eindhoven campus. This laboratory is part of the department of Biomedical Engineering. The laboratory of Chemical Biology consists of catagories two laboratories. The first one is an ML-I laboratory with biosafety level 1 (WHO BSL 1). Most of the work at the ML-I lab involves recombinant expression of proteins in E. coli or yeast expression systems and the application of bacteriophages for phage display. The second one is an ML-II laboratory (cell lab) with biosafety level 2 (WHO BSL 2). Work in the ML-II lab typically involves the use of immortalized cell lines, but it can also involve tissues or primary cells from animals or humans.
Figure 1. Plan of the Laboratory of Chemical Biology.
Safety Equipment
The basic protection equipment that we use in the wet lab are lab coats, safety goggles and gloves. Lab coats and safety glasses are alway sused and everywhere in the lab (ML-I and ML-II). Gloves (mostly latex) are used when handling chemicals and for working DNase free. In the cell lab (ML-II) they are also used for working with biological materials in the biosafety cabinets. Nitrile gloves are used for handling chemicals that pass through latex or for persons allergic to latex. Cryo-gloves and heat-resistant gloves are used for handling cold and hot materials respectively. Also when working with hazardous or toxic fumes used the fume hood.
Figure 2. Safety equipment
Working Equipment
We used a lot of equipment during our project, but the most important equipment are standard equipment like pipettes and pipettes tips to measure and transport liquids, but also the Bunsen Burner to work sterile and the centrifuge to spin the samples. Also other equipment is used like the PCR machine for DNA amplification and gel electrophoresis apparatus for gel extraction.
Figure 3. Working equipment
Figure 4. Open top bench with amongst other pipets , pipets tips, centrifuge, Bunsen burner and PCR machine.
Figure 5. Gel electrophoresis and SDS-page place.
Figure 6. Storage place for chemicals.
Figure 7. High speed centrifuge and other lab materials.
Figure 8. Working in the lab.
