Team:TU Eindhoven/Achievements/Submitted Parts/COMPx

From 2014.igem.org

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<p>CPX, or Circularly permuted OmpX, was developed as a bacterial display methodology for N- and C- terminal display. It has been shown to enable rapid screening of very large peptide libraries with high precision and efficiency. OmpX possesses four extracellular loops, with loops 2 and 3 forming a semi rigid β-sheets protruding from the cell surface. The native N- and C-termini were fused together by a GGSG linker, and the newly formed N and C termini reside on the cell surface.
<p>CPX, or Circularly permuted OmpX, was developed as a bacterial display methodology for N- and C- terminal display. It has been shown to enable rapid screening of very large peptide libraries with high precision and efficiency. OmpX possesses four extracellular loops, with loops 2 and 3 forming a semi rigid β-sheets protruding from the cell surface. The native N- and C-termini were fused together by a GGSG linker, and the newly formed N and C termini reside on the cell surface.
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Revision as of 13:55, 14 October 2014

iGEM Team TU Eindhoven 2014

iGEM Team TU Eindhoven 2014

COMPx

Figure 1. Structure of OmpX alongside a topological depiction of OmpX and CPX.

Usage and Biology

CPX, or Circularly permuted OmpX, was developed as a bacterial display methodology for N- and C- terminal display. It has been shown to enable rapid screening of very large peptide libraries with high precision and efficiency. OmpX possesses four extracellular loops, with loops 2 and 3 forming a semi rigid β-sheets protruding from the cell surface. The native N- and C-termini were fused together by a GGSG linker, and the newly formed N and C termini reside on the cell surface.

iGEM Team TU Eindhoven 2014