Team:TU Eindhoven/Achievements/Main Results

Main Results

Below a short point for point summary of the main results of our project is shown.

We have:

• developed two Clickable Outer Membrane Proteins, COMPx and COMPy.
• characterized the following properties of these proteins:
  • the SPAAC click reaction on the E. coli membranes.
  • the viability of E. coli cells expressing these proteins.
• improved an existing BBa K811005, Improved Biobrick: COMPy - K1492001 .
• delivered three new BioBricks to the registry (BBa K1492000, BBa K1492001 and BBa K1492003).
• tested two different molecular coatings on our cells:
  • PEG 5kDa and 10kDa
  • DNA combined with Rolling Circle Amplification
• successfully designed and developed microfluidic devices:
  • which enable the production of droplets in the desired size.
  • which allow fluorescent beads to be encapsulated.
• collaborated with the Rathenau Institute in the Synenergene project.
• written a children’s book used for educational purposes.
• undertook several outreach projects to spread knowledge on synthetic biology:
  • collaborated with the National Institute for Public Health (RIVM).
  • held an informal information evening for those interested.
  • actively reached out for press attention.
• made all our lab protocols available for future teams