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Team:TU Eindhoven/Achievements/Main Results
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<h2>Main Results</h2> | <h2>Main Results</h2> | ||
| - | <p></p> | + | <p><em>Below a short point for point summary of the main results of our project is shown.</em></p> |
| + | <p> | ||
| + | <br> | ||
| + | We have: | ||
| + | <ul style="color:white;margin-bottom:30px;list-style-type:disc;list-style-image:none;"> | ||
| + | <li>developed two <a href='https://2014.igem.org/Team:TU_Eindhoven/Design/Membrane_Anchor_Design'>Clickable Outer Membrane Proteins</a>, <a href="https://2014.igem.org/Team:TU_Eindhoven/Achievements/Submitted_Parts/COMPx">COMPx</a> and <a href="https://2014.igem.org/Team:TU_Eindhoven/Achievements/Submitted_Parts/COMPy">COMPy</a>.</li> | ||
| + | <li>characterized the following properties of these proteins: | ||
| + | <ul style="color:white;list-style-type:disc;list-style-image:none;"> | ||
| + | <li>the <a href='https://2014.igem.org/Team:TU_Eindhoven/Project/Characterization/Click_Coli'>SPAAC click reaction</a> | ||
| + | on the <i>E. coli</i> membranes.</li> | ||
| + | <li>the <a href='https://2014.igem.org/Team:TU_Eindhoven/Project/Characterization/Cell_Viability'>viability</a> of <i>E. coli</i> cells expressing these proteins.</li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li>improved an existing <a href='http://parts.igem.org/Part:BBa_K811005' target="_blank">BBa K811005, </a> Improved Biobrick: <a href='https://2014.igem.org/Team:TU_Eindhoven/Achievements/Submitted_Parts/COMPy'>COMPy - K1492001</a> .</li> | ||
| + | <li>delivered three new BioBricks to the registry (<a href="https://2014.igem.org/Team:TU_Eindhoven/Achievements/Submitted_Parts/COMPx">BBa K1492000</a>, <a href="https://2014.igem.org/Team:TU_Eindhoven/Achievements/Submitted_Parts/COMPy">BBa K1492001</a> and <a href="https://2014.igem.org/Team:TU_Eindhoven/Achievements/Submitted_Parts/tRNA">BBa K1492003</a>).</li> | ||
| + | <li>tested two different molecular coatings on our cells: | ||
| + | <ul style="color:white;list-style-type:disc;list-style-image:none;"> | ||
| + | <li><a href="https://2014.igem.org/Team:TU_Eindhoven/Project/Characterization/Click_Coli">PEG 5kDa and 10kDa</a></li> | ||
| + | <li><a href="https://2014.igem.org/Team:TU_Eindhoven/RCA">DNA combined with Rolling Circle Amplification</a></li> | ||
| + | </ul></li> | ||
| + | <li>successfully designed and developed <a href="https://2014.igem.org/Team:TU_Eindhoven/Microfluidics/Introduction">microfluidic devices:</a> | ||
| + | <ul style="color:white;list-style-type:disc;list-style-image:none;"> | ||
| + | <li>which enable the <a href="https://2014.igem.org/Team:TU_Eindhoven/Microfluidics/Results">production of droplets</a> in the desired size.</li> | ||
| + | <li>which allow <a href="https://2014.igem.org/Team:TU_Eindhoven/Microfluidics/Results_Cell_Encapsulation">fluorescent beads to be encapsulated.</a></li> | ||
| + | </ul></li> | ||
| + | <li>collaborated with the Rathenau Institute in the <a href="https://2014.igem.org/Team:TU_Eindhoven/Synergene/Techno-Moral_Vignets">Synenergene</a> project.</li> | ||
| + | <li>written a <a href="https://2014.igem.org/Team:TU_Eindhoven/Society/Policy_Practices/Children">children’s book</a> used for educational purposes.</li> | ||
| + | <li>undertook several outreach projects to spread knowledge on synthetic biology: | ||
| + | <ul style="color:white;list-style-type:disc;list-style-image:none;"> | ||
| + | <li>collaborated with the <a href="https://2014.igem.org/Team:TU_Eindhoven/Society/RIVM">National Institute for Public Health</a> (RIVM). </li> | ||
| + | <li>held an <a href="https://2014.igem.org/Team:TU_Eindhoven/Society/Outreach/Informal_Information_Evening">informal information evening</a> for those interested.</li> | ||
| + | <li>actively reached out for <a href="https://2014.igem.org/Team:TU_Eindhoven/Society/Outreach/Media_Attention">press attention</a>. | ||
| + | </li> | ||
| + | </ul></li> | ||
| + | <li>made all our <a href="https://2014.igem.org/Team:TU_Eindhoven/Protocols">lab protocols</a> available for future teams </li> | ||
| + | </ul></p> | ||
</div> | </div> | ||
Latest revision as of 00:47, 18 October 2014
Main Results
Below a short point for point summary of the main results of our project is shown.
We have:
- developed two Clickable Outer Membrane Proteins, COMPx and COMPy.
- characterized the following properties of these proteins:
- the SPAAC click reaction on the E. coli membranes.
- the viability of E. coli cells expressing these proteins.
- improved an existing BBa K811005, Improved Biobrick: COMPy - K1492001 .
- delivered three new BioBricks to the registry (BBa K1492000, BBa K1492001 and BBa K1492003).
- tested two different molecular coatings on our cells:
- successfully designed and developed microfluidic devices:
- which enable the production of droplets in the desired size.
- which allow fluorescent beads to be encapsulated.
- collaborated with the Rathenau Institute in the Synenergene project.
- written a children’s book used for educational purposes.
- undertook several outreach projects to spread knowledge on synthetic biology:
- collaborated with the National Institute for Public Health (RIVM).
- held an informal information evening for those interested.
- actively reached out for press attention.
- made all our lab protocols available for future teams
