Team:TU Eindhoven

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                  <h2>Click Coli</h2>
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<p style="margin-top:-20px;font-size:20pt;"><em>Expanding the Chemical Toolbox for Bacteria</em><p>
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                  <p>iGEM Team TU Eindhoven 2014 focuses on engineering the outer membrane of <br><i>E. coli</i>. We have created a new tool for the synthetic biologist&rsquo;s toolbox; a tool that allows genetically engineered machines to have a specific coating. Want to know more of our project? Go to our <a href="https://2014.igem.org/Team:TU_Eindhoven/Project_Description">Project Description</a> page! For a brief overview of the Click Coli system, check our <a href="https://2014.igem.org/Team:TU_Eindhoven/Overview">Overview Click Coli</a>! Also watch our animation of Click Coli below!</p>
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                  <h2>Contact</h2>
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                  <p>If there are any questions considering this iGEM Project, please feel free to contact us. Below is the general contact information. Check our <a href="https://2014.igem.org/Team:TU_Eindhoven/Team_Members">Team Page</a> for individual contact information.</p>
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                    <li><a href="http://www.google.nl/maps/place/51%C2%B026'49.3%22N+5%C2%B029'19.4%22E/@51.447528,5.4891113,17z/data=!4m2!3m1!1s0x0:0x0%22%22" class="service_one" target="_blank">Eindhoven University of Technology<br />  
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Den Dolech 2, 5612 AZ Eindhoven<br />  
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<h2>TU Eindhoven iGEM 2014 Project</h2>
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The Netherlands<br />  
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<p>Our goal this year in the iGEM competition is to create a new tool for the synthetic biologist’s toolbox; a tool that allows genetically engineered machines to operate inside the human body without causing an immune response. We want to achieve this by combining polymer chemistry and microfluidics to create individually encapsulated bacteria. Want to learn more? Go to our <a href="https://2014.igem.org/Team:TU_Eindhoven/Project"> Project Overview</a>!</p>
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Ceres 0.04</a></li>
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                    <li><a href="mailto:IGEM@tue.nl" class="service_two" style="background:url(https://static.igem.org/mediawiki/2014/8/85/Mail_TU_Eindhoven.png) center left no-repeat;">iGEM@tue.nl</a></li>
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<h2>Microfluidics</h2>
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<p>We are busy optimizing our droplet device to get the right parameters for our final device. The design for this device is also being  finalized. The device will be used to create droplets with a few bacteria inside in which the polymer chemistry will be done. After the reaction we will test the formation of the encapsulation with FACS. For more information on the subject of Microfluidics, check our <a href="https://2014.igem.org/Team:TU_Eindhoven/Project/Microfluidics"> Microfluidics Page</a>!</p>
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<h2>Lab Work</h2>
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<p>Currently we are making our designed plasmids, after the sequencing results we will hopefully be able to start with protein expression and turning our designed genes into biobricks! See the <a href="https://2014.igem.org/Team:TU_Eindhoven/Project/Labwork"> Lab Work Page </a> for more information about our achievements in the lab!</p>
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<h2>Modeling</h2>
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<p>We are currently busy researching possible modelling projects, come back later for more information! </p>
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<h2>Policy & Practices</h2>
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<p>For Policy & Practices two big projects are currently in focus: creating a picture book about lab work for our younger audience, and building a game about synthetic biology for middle school students. </p>
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<p class="top"><a href="https://2014.igem.org/Team:TU_Eindhoven/Microfluidics"> Microfluidics</a>: Tests with our cell encapsulation devices are currently performed with use of fluorescent beads as a replacement of bacterial cells.</p>
 
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<p class="top"><a href="https://2014.igem.org/Team:TU_Eindhoven/Microfluidics"> Microfluidics</a>: Testing with polyacrylamide droplets is going well.</p>
 
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<p class="top"> <a href="https://2014.igem.org/Team:TU_Eindhoven/Labwork"> Lab Work</a>: Click reaction results show that the product forms, optimization of the reaction is now underway. </p>
 
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<p class="top"><a href="https://2014.igem.org/Team:TU_Eindhoven/Labwork"> Lab Work</a>: FACS analysis using fluorescent DBCO molecules shows that our modified protein is expressed on the membrane, we are now testing with DBCO molecules with PEG tails.</p>
 
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<p class="btm1 pin"><a target="_blank" href=http://www.google.nl/maps/place/51%C2%B026'49.3%22N+5%C2%B029'19.4%22E/@51.447528,5.4891113,17z/data=!4m2!3m1!1s0x0:0x0"">Eindhoven University of Technology <br> Den Dolech 2, 5612 AZ Eindhoven <br> The Netherlands <br> Ceres 0.04</a></p>
 
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<p class="btm1 mail"><a href="mailto:IGEM@tue.nl">iGEM@tue.nl</a></p>
 
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Latest revision as of 23:56, 17 October 2014

iGEM Team TU Eindhoven 2014

iGEM Team TU Eindhoven 2014

Click Coli

Expanding the Chemical Toolbox for Bacteria

iGEM Team TU Eindhoven 2014 focuses on engineering the outer membrane of
E. coli. We have created a new tool for the synthetic biologist’s toolbox; a tool that allows genetically engineered machines to have a specific coating. Want to know more of our project? Go to our Project Description page! For a brief overview of the Click Coli system, check our Overview Click Coli! Also watch our animation of Click Coli below!

Contact

If there are any questions considering this iGEM Project, please feel free to contact us. Below is the general contact information. Check our Team Page for individual contact information.

iGEM Team TU Eindhoven 2014

Retrieved from "http://2014.igem.org/Team:TU_Eindhoven"