Team:TU Eindhoven

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Micro-encapsulation
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<title>iGEM Team TU Eindhoven 2014</title>
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This year we, the TU Eindhoven team, will be trying to create bacteria that are able to synthesize their own encapsulation that will protect them from the human body’s immune system. In order to do so, the bacteria will have to be able to create a biopolymer with high anti-fouling properties and attach that to their membranes. We are currently researching what the best way is to achieve this encapsulation, so stay tuned to our website and Facebook page to see the latest updates on our project!
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Current solution
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Bacteria can be made undetectable to the immune system with the use of encapsulation. Hydrogels are suitable for this purpose. Hydrogels are water-absorbing (synthetic) polymers, and are therefore able to form a layer around the bacteria. Due the low reactivity of this hydrogel capsule towards the immune system and permeability to small essential molecules (for example nutrients and wastes). With the use of microfluidic techniques, the amount of bacterial cells per liquid droplet is relatively easy to control. In the produced microfluidic droplet, hydrogel formation can be induced. The result is a hydrogelation from the outside towards the core of the droplet, surrounding the entire group of bacteria. The problems with these current techniques are: inhomogeneous hydrogelation (due to gelation form the outside to the core), uncontrollable cell growth inside the encapsulation, thus incontrollable drug release, and non-degradable encapsulations.
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                  <h2>Click Coli</h2>
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<p style="margin-top:-20px;font-size:20pt;"><em>Expanding the Chemical Toolbox for Bacteria</em><p>
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                  <p>iGEM Team TU Eindhoven 2014 focuses on engineering the outer membrane of <br><i>E. coli</i>. We have created a new tool for the synthetic biologist&rsquo;s toolbox; a tool that allows genetically engineered machines to have a specific coating. Want to know more of our project? Go to our <a href="https://2014.igem.org/Team:TU_Eindhoven/Project_Description">Project Description</a> page! For a brief overview of the Click Coli system, check our <a href="https://2014.igem.org/Team:TU_Eindhoven/Overview">Overview Click Coli</a>! Also watch our animation of Click Coli below!</p>
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                  <h2>Contact</h2>
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                  <p>If there are any questions considering this iGEM Project, please feel free to contact us. Below is the general contact information. Check our <a href="https://2014.igem.org/Team:TU_Eindhoven/Team_Members">Team Page</a> for individual contact information.</p>
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                    <li><a href="http://www.google.nl/maps/place/51%C2%B026'49.3%22N+5%C2%B029'19.4%22E/@51.447528,5.4891113,17z/data=!4m2!3m1!1s0x0:0x0%22%22" class="service_one" target="_blank">Eindhoven University of Technology<br />
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Den Dolech 2, 5612 AZ Eindhoven<br />
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The Netherlands<br />
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Ceres 0.04</a></li>
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                    <li><a class="service_three">+31 40 247 55 59</a></li>
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Latest revision as of 23:56, 17 October 2014

iGEM Team TU Eindhoven 2014

iGEM Team TU Eindhoven 2014

Click Coli

Expanding the Chemical Toolbox for Bacteria

iGEM Team TU Eindhoven 2014 focuses on engineering the outer membrane of
E. coli. We have created a new tool for the synthetic biologist’s toolbox; a tool that allows genetically engineered machines to have a specific coating. Want to know more of our project? Go to our Project Description page! For a brief overview of the Click Coli system, check our Overview Click Coli! Also watch our animation of Click Coli below!

Contact

If there are any questions considering this iGEM Project, please feel free to contact us. Below is the general contact information. Check our Team Page for individual contact information.

iGEM Team TU Eindhoven 2014

Retrieved from "http://2014.igem.org/Team:TU_Eindhoven"