Team:TU Darmstadt/Notebook/Labjournal/K1497033

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Scaffold GSP

The scaffold GSP was designed in silico with GS linker groups between the single domains. Additionally, a BamHI restriction site was added at the C-terminal of the protein.  Its sequence was codon optimized to improve the ability of the scaffold to be expressed in E. coli and then synthesized via solid phase DNA-synthesis. For the generation of a new Biobrick the sequence was cloned into pSB1C3.