CFP is a reporter, which we got out the registry (E0020).  We cut the E0020-plasmid with XbaI + PstI and cut the Naringenin sensor plasmid with SpeI + PstI. In addition, we dephosphorylized the digestion of the naringenin sensor. Subsequently we ligated the insert into the backbone and behind the Naringenin Sensor using T4 ligase, producing a mixed site between the sensor and the reporter. We transformed the ligation product into Top10 E. coli via heatshock and spread them out on CMP-LB agar plates. We incubated the plates overnight at 37°C and analyzed obtained colonies by colony-PCR and agarose gel electrophores. Positive colonies were inoculated in 5 ml LB with CMP and plasmids were isolated after incubating for 16 h at 37°C. For further verification we send the samples to Eurofins for sequencing.