We kindly received the CHS gene from Dr. Stefan Martens. We introduced Biobrick prefix and suffix and amplified our part of interest via plasmid PCR using the primers "CHS_psb1c3_Pre2" and "CHS_pscb1c3_Suf2"  Subsequently we cleaned up the PCR product by DpnI digestion and Wizard PCR Clean Up Kit. Afterwards we cut the PCR product with EcoRI + PstI and cloned the insert into a previously digestion of mRFP-pSB1C3 with EcoRI + PstI. We used T4 ligase for ligation and transformed the ligation into Top10 E.coli via heat shock. For spreading out we used CMP-LB agar plates. After incubating overnight at 37°C we analyzed obtained colonies by colony-PCR and agarose gel electrophoresis. We inoculated positive colonies in 5 ml LB with CMP. After incubating for 16 h at 37°C we isolated the plasmids gaining the BioBrick BBa_K1497001.
For further verification we send the samples to Eurofins for sequencing.