Safety

1. Your Training

• Have your team members received any safety training yet?
Yes, we have already received safety training.
• Please briefly describe the topics that you learned about (or will learn about) in your safety training.
We have recieved a lecture by KIT staff for half a day.It was about gene operation,gene conservation and the way of disposing the recombinants.
• Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.
http://www.kit.ac.jp/01/prescription/act/frame110000126.htm

2. Your Local Rules and Regulations

• Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety, a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.
The charge of biological safety is professor Masanobu Ito.We had a discussion and our plan was OK,so we didn't have to change our plan.
• What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.
http://www.lifescience.mext.go.jp/bioethics/anzen.html#kumikae
• In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link .
http://www.lifescience.mext.go.jp/bioetics/kankeihourei.html http://www.lifescience.mext.go.jp/bioetics/anzen.html#kumikae

3. The Organisms and Parts that You Use

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Details

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Materials & Methods

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Result

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Species name (including strain)	Risk Group	Risk Group Source	Disease risk to humans?	Part number/name	How did you acquire it?	How will you use it?
d-limonene synthase gene(Citrus unshu)	1	NIH Guidenines	no risks	AB110636/CuMTSE1	Synthase d-limonene	We got it from Dr.Shimada, NARO(National Agriculture and Food Research Organization)	Inserting it into Escherichia coli
gamma-terpinene synthase gene(Citrus unshu)	1	NIH Guidenines	no risks	AB110639/CuMTS3	Synthase gamma-terpinene	We got it from Dr.Shimada, NARO(National Agriculture and Food Research Organization)	Inserting it into Escherichia coli Inserting it into Escherichia coli
beta-pinene synthase gene(Citrus unshu)	1	NIH Guidenines	no risks	AB110641/CuMTS62	Synthase beta-pinene	We got it from Dr.Shimada, NARO(National Agriculture and Food Research Organization)	Inserting it into Escherichia coli