Team:LZU-China/Parts

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<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> LZU-China 2014

SUMMARY OF THE PARTS

No	Part No.	NCBI No.	Resistance	Vector	Gene Name	Gene function	Length	Website (NCBI)
1	BBa_K1523021	NC_004999.1	Chl	pSB1C3	nahR	nahE coding sequence, encoding a LysR-type transcriptional regulator	903 bp	http://www.ncbi.nlm.nih.gov/gene/1343208
2	BBa_K1523022	NC_007926.1 (37386..38381)	Chl	pSB1C4	nahE	nahE coding sequence	1005 bp	http://www.ncbi.nlm.nih.gov/gene/3974214
3	BBa_K1523023	NC_007926.1 (44492..45796)	Chl	pSB1C5	nahG	nahG coding sequence	1305 bp	http://www.ncbi.nlm.nih.gov/gene/3974216
4	BBa_K1523025	NC_017530.1	Chl	pSB1C6	chrR	chrR coding sequence	666 bp	http://www.ncbi.nlm.nih.gov/gene/12519943
5	BBa_K1523101	N/A	Chl	pSB1C7	PyeaR	PyeaR->Riboflavin synthase	3726 bp	N/A
6	BBa_K1523008	NC_007779.1	Chl	pSB1C8	Yief	yieF coding sequence	560 bp	http://www.ncbi.nlm.nih.gov/gene/12933177

PARTS SEQUENCES

ATGGAACTGCGTGATCTGGATTTAAACCTGCTGGTGGTGTTCAACGAGCTGCTGGTCTGCAGACGCGTCTCTATCGTTGCCGAGAACCTGGGCCTGACCCAGCCTGCTGTCAGCAATGCGCTGAAACGCCTGCGCACAGCGCTACAGGACCAACTTTTCGTGCGCACCTACCAGGGTATGGAACCCACACCCTACGCCGCGAACTTGGCCGAGCCCGTCGCCTTGGCCATGCACGCCCTGCGCGAAGCCCTGCAGCACCATGAGCGCTTCGATCCGCTGACCAGCGAGCGTACTTTCACCCTGGCCATGACCGACATCGGCGAGATCTACTTCATGCCGCGGCTGATGGATGCGATTACTCTTCAAGCCCCCAATTGCGCGATCAGCACCGTGCGCGACAGTTCGATGAGCCTGATGCAAGCCTTGCAGAACGGCACAGTGGACCTAGCCGTGGGCCTGCTGCCCAACCTGCAGACTGGCTTCTTCCAGCGCCGGCTGCTCCACAATCACTACGTGTGCCTGTGTCGCAAGGACCATCCGGCCACCCGCGAACCCCTGACTCTTGAGCGCTTCTGTTCCTATGGCCACGTGCGTGTCATCGCCGCTGGCACAGGCCACGGCGAGGTGGACACATACTTGACGAAGGCCGGCATCCGGCGCGACATCCGCCTGGAGGTGCCGCACTTCGTCGCCGTTGGCCACATACTCCAGCGCACCGAGCTGATCGCCACTGTGCCGATACGTTTCGCCGACTGCTGCGTAGAGCCCTTCGGTCTGAGCGTCTTGCCGCACCCAGTCGCCTTGCCGGAAATCGCCATCAACATGTTCTGGCATGCGAAATACCACCAGGACCTCGCCAATATCTGGTTGCGGCAACTGATGTTCGACCTGTTTTCTGATTGA

>nahE trans-o-hydroxybenzylidenepyruvate hydratase-aldolase 1005bp
ATGTCGAATAAAATTATGAAAACGTCGCGTCTTACCGCCGAAGATATCAACGGCGCCTGGACTATAATGCCCACACCCTCGACGCCTGATGCTTCTGATTGGCGCAGCACTGCCACCGTGGACTTAGAAGAGACTGCCCGCATAGTTGAAGAGCTGATTGCAGCTGGTGTCAACGGTATTCTAAGTATGGGTACTTTTGGTGAGTGCGCCACGTTGACCTGGGATGAAAAACGTGATTATGTCTCGACGATTGTCGAGACCATTCGTGGTCGCGTGCCTTATTTCTGTGGCACGACAGCCTTAAATACCCGAGAAGTCATCCGCCAGACCCGAGAGCTTATCGATATTGGCGCCAACGGCACTATGCTCGGGGTGCCGATGTGGGTGAAGATGGACCTGCCTACAGCGGTTCAGTTCTATCGTGATGTTGCAGATGCGGTACCAGAGGCTGCCATTGCGATTTACGCCAACCCCGAAGCATTCAAGTTCGACTTCCCTCGCCCATTCTGGGCAGAGATGTCCAAAATTCCGCAGGTAGTGACTGCGAAGTATCTAGGCATCGGAATGCTTGACTTGGACCTGAGACTGGCACCCAACATCCGCTTCCTTCCCCACGAAGATGACTATTACGCGGCCGCACGCATCAATCCCGAGCGCATAACCGCGTTCTGGTCAAGCGGGGCCATGTGCGGCCCGGCTACCGCCATCATGTTGCGTGACGAAGTGGTGCGGGCCAAGAGCACCGGTGACTGGGCCAAGGCCAAAGCCATCTCCGATGATATGCGTGCAGCCGACTCGACATTGTTTCCGCGTGGCGACTTTTCGGAGTTCTCGAAGTATAATATCGGGCTTGAAAAGGCACGGATGGATGCGGCTGGTTGGCTCAAGGCTGGGCCCTGCCGTCCGCCCTACAACCTTGTTCCAGAAGACTACCTCGCTGGTGCACAGAAATCAGGCAAGGCTTGGGCCGCGCTGCACGCTAAATACAGTAATGAATTGAAGTAG

>nahG salicylate hydroxylase 1305bp
ATGAAAAACAATAAACCTGGCTTGCGCATCGGTATCATCGGCGGCGGGATTTCCGGCGTTGCCTTGGCTCTGGAGCTCTGTCGCTACTCCCATCTCCAAGTACAGCTGTTCGAGTGCGCGCCGGCTTTTGGTGAGGTCGGTGCAGGAGTTTCCTTTGGCCCCAACGCGGTGCGCGCCATTGTCGGCCTAGGCTTGGGCGAGGCCTACTTGCAGGTCGCCGACCGTACCTCGGAGCCCTGGGAGGACGTGTGGTTCGAGTGGCGGCGCGGCAGGGATGCCAGCTATCTGGGAGCGACCATCGCTCCGGGCGTGGGCCAGTCCTCGGTACACCGGGCGGATTTCCTCGATGCCCTAGTAAATCACCTCCCAAAAGGTATCGCCCAATTCAGGAAGCGCGCCACACAGGTCGAGCAAAAGGGGGGCGAAGTGCAAGTGCTATTCGCCGACGGCACAGAGCACCGCTGTGACCTTCTAATCGGTGCCGACGGAATCAAGTCAGCGCTGCGTAGTCATGTGCTGGAAGGTCAGGGGCTGGCCCCACAGGTACCGCGCTTCAGCGGCACCTGTGCCTATCGGGGGATGGTCGACAGCCTACACCTGCGCGAAGCCTATCGAGCCCAGGGCATCGACGAGCACTTGGTGGACGTACCGCAGATGTACCTAGGGCTTGACGGCCATATCCTCACTTTTCCGGTGAGGAATGGCCGCCTCATCAACGTTGTGGCCTTCATTTCCGACCGTAGTGAGCCGAAGCCGAACTGGCCCGCGGATGCCCCTTGGGTGCGCGATGTGAGCCAGCGAGAGATGCTCGATGCCTTCGCGGGTTGGGGTGATGCCGCGCGCACCCTGCTGGAGTGCATCCCGGCACCAACTCTCTGGGCACTGCACGACCTGGCGGAGCTGCCGGGCTACGTGCACGGGCGGGTCGTCCTGATCGGCGACGCAGCTCACGCCATGCTGCCGCACCAAGGCGCCGGTGCCGCCCAAGGACTTGAGGACGCCTACTTCCTCGCCCGCCTGTTGGGCGATACCCAAGTCGATGCAGACAACCTCGCCGAGCTGCTTGAAGCCTACGACGACCTGCGCCGCCCTCGTGCCTGTCGCGTGCAGCGAACCTCCCGGGAGACCGGCGAGTTATACGAGTTTCGCGACCCCGTCGTAGGTGCGAACGAGCATCTGCTGGGGGAAAACCTGGCGACCCGCTTCGACTGGCTGTGGAGCCACGACCTCGACGCCGACCTGGCCGAGGCACGTGCGCGCCTGGGTTGGGAAAATGGTAGCCGGGGTGTGCTACGTCAAGGGTGA

>ChrR chromate reductas 666 bp
ATGAAAAAAATAGTCCAGTCGGAAGGTTTTCGGTTCTCCGCTGGTCCAATTGTTCGCCCGATCATCTATAAATGGGGCTCCCTTGAACCCATCGTCGAGAAGTGTCCAATGAGTAATGTCTACACCGTGGCCGTTTTAGTGGGGAGCCTGCGCAAGGCTTCGATCAATCGCAAGCTGGCTCTGGCCCTGGCGGATCTGGCGCCGAGCAACCTGAAGCTGGAAATCATCGAGATAGGCGACCTGCCGCTCTACAACGAAGATATTGACGTCACACCCCCACCAGCCTACAGCACTTTCCGTGAGCAGGTTGGCGCGGCCGACGCATTGCTGTTTGTCACGCCTGAATACAACCGCTCGGTTCCGGCCCCGATGAAAAATGCCATTGACGTCGGCTCGCGGCCTTACGGCAAGAGCTGCCTGAGCGGTAAACCGGGTGCGGTGATCAGTGCCTCGCCGGGAGCCATGGGTGGTTTCGGCGCCAATCATCATTTGCGCCAGTCCCTGGTTTTCCTCGATGTGCCTTGCATGCAACAGCCGGAGGCTTATCTGGCCGGTGCGGGGAGCGCGTTCGATGAGCAGGGCGAGCTGTCTGAGTCGGTGAGGCCGTTTCTGCAGAAATTCATTGATGCCTATGGCAAGTGGGTTGAACAGCACAAAAAGGCCTGA

>BBa_K1523101 Part-only sequence (3726 bp)
TTCCCATCTATAATCCTCCCTGATTCTTCGCTGATATGGTGCTAAAAAGTAACCAATAAATGGTATTTAAAATGCAAATTATCAGGCGTACCCTGAAACG
TACTAGAGATTAAAGAGGAGAAATACTAGATGAATTGGTCAGTACTCGATAACCAAATGATGAGCCGAGCTATTCAATTGGCTCGCAAAGGATTTTATAC
CACTCGCCCCAATCCCAGTGTGGGCTGCGTTATCGTTAACGATAACCAGATTGTCGGTGAAGGTTATCATCAAAAAGCCGGAGAGCCCCACGCTGAAGTG
CATGCGCTGCGCATGGCTGGCGAGCTTGCCCGTGGCGCGACTGCTTATGTCACCTTAGAACCCTGTAGCCATTATGGTCGTACGCCGCCCTGCGCCTTGG
CGCTGATCAATATTGGTGTAAAGCGGGTGGTGGTGGCGGTTGAAGATCCTAATCCGCAGGTCGGCGGGCGCGGTATTCAAATGCTGCGCGATGCGGGTAT
TCAAGTGGATGTGGGCTTGCATCGTGATGAGGCCTATGCCTTAAATCTTGGTTTTATGAAGCGCATGGAGTCGGGTCTACCTTGGGTGACGGTAAAGCTT
GCCGCGAGTCTTGATGGTAAAACTGCGTTATCAAACGGGGTGTCTAAATGGATCACTGGCCCTGAAGCTCGCCGCGATGTGCAGCGCTTAAGATTACGAT
CCTGCGCGCTGATCACGGGGATTGAAACGGTACTGGCCGATGATCCATCGCTCAATGTGCGCTACTCAGAGCTTGGCAGTTTAAGAGCGCAATTGAGTGA
GTCGCAGGTTTTACAGCCGCTGCGAGTGGTTTTGGACAGTCGCGCCCGCATGCCTATCACGGCAGCATTGTTTGCGATTGAATCACCCATTTTATTAGTC
TCGACAGTGAGCTATTCGTCAGACTTTATGGTGCAATTACCCGCCCATGTGACTTGCCTCCAATTACCAGCCATTGAGGGTCGAATCTCCTTGTCTGCAC
TATTAAGCTATTTAGGCAAAAGCTGTAATCAGGTGCTTATCGAAGCGGGCGCGACTTTAGCTGGCGCCTTTATCGGCTCAGGATTAGCCGATGAATTAGT
GCTTTATCAAGCGATGAAAATCCTTGGCGGCCAAGGACGTAATCTACTTGAATTACCCGATTATCAAATGATGGCCGATATTCCGACCCTCAAATTGGTC
GACGAGCGTAAAGTGGGCGCGGATACGCGCTTCACCTTGAGGCTCGAGTCCAATCCATCTTTAGCGAATAAGTGAGTTAACCATGTTTACTGGGATTATT
GAGGCCGTTGGTACACTGCGAAAGCTTGAGCGTAAAGGCGATGATATTCGCTTAACGGTCGCCAGTGGCAAACTGGATTTAACCGATGTGCGTTTAGGCG
ACAGTATCGCCACCAACGGTGTATGTTTGACCGTGGTTCAACAGTTAGCCGATGGCTATGTGGCTGATGTGTCAGCTGAAACCGTCAGCCTTACAGGTTT
TGCAAACTATAAAGTGGGTACTAAGGTCAATCTTGAAAAGGCCGTCACCCCAACTACTCGTTTAGGCGGCCATATGGTGAGTGGTCATGTGGATGGGATT
GCTACTGTTGAGCAGCGTCTTGCTCGTGGTCAAGCAATCGAATTTTGGTTAGCGGCTCCCACGGAGCTTGCTCGCTATATTGCCCATAAAGGTTCTATCA
CGATTGATGGCGTGAGCCTGACAGTAAACGAAGTCGATGGACACCGTTTTCGATTAACGATTGTGCCCCATACTGTAGGTGAAACCACGTTGGTGGATTT
AAAAGCAGGCGATAAGGTCAATATTGAAGTGGACTTAATTGCCCGTTATTTAGAGCGTTTAATGCGTTTTGACAGCAAAGAAACCCAAGGCGGAGGGGTG
ACCATGGAAATGTTAGCCCGTGCTGGCTTTGTGCGTTAGTACACTGGCACTCAGTTCACTTAGGTATAGAATTTATAACAACAGTCACATTATAAAGGTC
CTACAATGGCGCTGCACAGTATAGAAGAGATCATCGAGGATATTCGTCAAGGCAAAATGGTTATTTTGATGGATGACGAGGACAGAGAAAACGAAGGCGA
TCTGATTTTGGCGGCCGAAATGGTGACGCCAGAAGCGATTAACTTTATGGCGAAATACGGCCGCGGGTTAATCTGCCAAACGATGACCAAGGCCCGTTGC
CAGCAGTTAAATCTGCCCTTGATGGTAACCAATAACAATGCGCAGTTCTCGACCAACTTTACGGTATCGATTGAAGCTGCCGAAGGCGTAACCACAGGGA
TTTCGGCCCACGATCGCGCTGTAACCGTTAAAGCGGCCGTAGCAAAAGATGCTAAAGCGTCGGACTTAGTTCAGCCTGGGCATATCTTCCCGTTAATGGC
GCAGGATGGTGGCGTATTAACCCGCGCAGGTCACACTGAAGCGGGCTGTGATTTAGCCCGTTTAGCAGGGCTTGAACCATCGGGGGTGATTGTTGAAATT
TTGAACGAAGACGGCACTATGGCCCGTCGCCCAGATTTAGAGATTTTCTCTGAAAAACATGGCATTAAAATCGGCACTATTGCGGCCCTGATTGAATACC
GTAATACCAAAGAAACGACCGTTGTGCGTGAAGCCAAATGCAAACTGCCAACCCGTTTCGGTGAGTTCGATATGGTGACCTTTAGGGATACTATCGACAA
TCAATTGCACTTTGCCTTAGTGAAAGGCGAGGTGAAGCCTGACTGTTTAGTACGGGTACATTTACAGAATACCTTTAACGATCTACTTCACTCTGAGCGT
GATCAGCAACGTAGCTGGCCGCTCGAAAAAGCGATGGCGCGGATTTCTGCCGAAGGTGGTGTATTAGTACTGCTTGGCAATCAGGAACATTCCAGTGAAA
TCCTCGCCAAAGTCAAAGCCTTTGAAGCGGAAGATCAAGGTCAGACCCCAGTATCGGCTAAATGGCAAGGCACCTCACGCCGTGTAGGTGTGGGTTCGCA
AATCCTCGCCAGTTTAGGCGTGACAAAAATGCGACTCTTAAGCTCGCCCAAACGTTATCACTCACTTTCAGGCTTTGGCCTTGAAGTGACCGAATGCGTA
GCTGAGTAACAGGTTAAGCTTGTAATAGGTTGCACTAAGGTAAAATTAACCATTTCTATCATTTGCATAAGGCCGCGGGCAATTGACTGTGGTATCATGT
CGCCACTTTTCGCCCAGAGCTGGGTGCTTTAGCTAAATTAGGTAAGAAAATGAACGTAGTTCAAGGTAATATCGAAGCGAAGAATGCCAAAGTTGCGATT
GTAATTTCGCGTTTTAACAGCTTTTTAGTCGAAAGCCTGCTTGAAGGTGCACTTGACACGCTGAAACGTTTTGGCCAAGTCAGTGATGAAAACATCACTG
TTGTCCGTGTACCTGGTGCGGTTGAGTTACCGCTGGCTGCACGTCGTGTTGCCGCAAGTGGTAAGTTTGACGGTATTATTGCACTTGGTGCGGTAATCCG
TGGTGGTACCCCTCACTTTGATTTTGTTGCAGGCGAATGTAACAAAGGTCTGGCTCAAATTGCATTAGAGTTCGATCTGCCCGTTGCTTTCGGTGTATTG
ACTACAGATACCATTGAACAAGCTATTGAGCGTTCAGGTACTAAAGCGGGTAACAAAGGCGGCGAAGCTGCTCTAAGCTTGCTTGAAATGGTCAACGTTC
TGCAACAGCTTGAACAACAGTTGTAA

>yieF chromate reductas 560 bp
ATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTG
CACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCC
CTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATC
CGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATG
CCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCGGTATTGATTCAGACCAGCTC
AATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATG
GTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGA
TTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAA
GATCTAA

GEL PICTURE OF SOME PARTS

Fig.2 The picture of K1523101. 1,3: pSB1C3 with K1523101 cut by EcoRI; 2,4: pSB1C3 with K11523101 cut by EcoRI and HindIII; 5: pSB1C3 with K1523101 not be cut.