Team:TU Darmstadt/Notebook/Labjournal/K1497012

From 2014.igem.org

Revision as of 23:10, 17 October 2014 by N.Hummel (Talk | contribs)

Home


Flavonon-3beta-hydroxylase with strong RBS (B0034-F3H)

Pathway

This part encodes the sequence for the enzyme Flavonon-3beta-hydroxylase (F3H; EC 1.2.7.3) from Petroselinum crispum with a strong ribosome binding site. To get a functional enzyme, we integrated a strong ribosomal binding site (RBS; B0034). For the RBS integration we used the K1497009 as template for a PCR with the following primers:

F3H_RBS_Prefix and F3H_Suffix.