After we created BBa_K1497010, a ribosom binding site had to be added to come a step further for creating a functioning expression system. For this purpose, we used BBa_K1497010 as a template for PCR with RBS primers (Prefix_RBS_DFR-primer and DFR_Suffix-primer). The PCR product was then restricted with the restriction enzymes EcoRI and PstI and ligated into an equaly restricted pSB1C3 vector to create the biobrick BBa_K1497013.