Team:SCUT/Project/Other Work

Leading Peptides for Future Plan

What we want to do next is to find the other pathways in the yeast and make it highly effective by taking advantages of the micro environments. But frist of all, how to make the protein import into its own station and work smoothly? Fortunately, we find some leading peptides to be our “guides”. As we know, different locations need different “guides”. This summer, we have constructed 6 leading peptides successfully in the yeast. In other words, we have 6 another possibility to take advantages of the micro environments.
We chose those 8 membrane from a list of about ten candidates based on the following factor:
1.It can be easily targeted in yeast.
2.Less reaction over it is not allowed.
3.Visible distinction from others.
So far，we have found the following leading peptides.

1.Erv25p
We characterize a novel protein termed Erv25p that have benn discovered on ER-derived transport vesicles, and it is required for efficient ER to golgi transport. Erv25p is a single membrane spanning segment derived from yeast, and a 12-amino acid N-terminal sequence exposed to the cytoplasm.

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2.CTS1-1

Figure1丨CTS1-1 targets to cytoderm丨N-terminal 12-amino acid serve as a typical cleavable signal sequence. The targeting sequence derived from yeast can be located at cytoderm.

A N-terminal 12-amino acid serve as a typical cleavable signal sequence. The targeting sequence derived from yeast can be located at cytoderm.
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3.CIIC
It’s a 12-amino acid C-terminal sequence separated from the Ras protein,which is the leading peptide of Plasma Membrane.

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4.H2A2
The localizing protein(H2A2) is the major structural protein of chromosomes. And the C-terminal protein fusion to it will be located at nucleus.

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5.ZRC1
The localizing protein(ZRC1）is a protein of vacuolar membrane zinc transporter.And a C-terminal protein fusion to it will be located at vacuolar membrance.

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6.PTS
There is a mechanism about cargo translocation in the peroxisomes, the matrix proteins are posttranslationally targeted to peroxisomes from the cytosol by peroxisomal targeting signals (PTSs). These signals include the predominantly used PTS1 and the less prevalent PTS2, which are recognized by the soluble import receptors PEX5 and PEX7, respectively.

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