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Team:TU Darmstadt/Notebook/Labjournal/K1497012
From 2014.igem.org
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| - | <!--TYPO3SEARCH_begin--><div id="c144" class="csc-default"><div class="csc-textpic-text"><p style="text-align:justify; line-height:150%"><span style="font-family:"Arial","sans-serif"" lang="EN-US">This part encodes the sequence for the enzyme Flavonon-3beta-hydroxylase (F3H; EC 1.2.7.3) from <i>Petroselinum crispum </i>with a strong ribosome binding site. To get a functional enzyme, we integrated a strong ribosomal binding site (RBS; B0034). For the RBS integration we used the <a href="K1497009" title="Opens internal link in current window" class="internal-link">K1497009</a> as template for a PCR with the following <a href=" | + | <!--TYPO3SEARCH_begin--><div id="c144" class="csc-default"><div class="csc-textpic-text"><p style="text-align:justify; line-height:150%"><span style="font-family:"Arial","sans-serif"" lang="EN-US">This part encodes the sequence for the enzyme Flavonon-3beta-hydroxylase (F3H; EC 1.2.7.3) from <i>Petroselinum crispum </i>with a strong ribosome binding site. To get a functional enzyme, we integrated a strong ribosomal binding site (RBS; B0034). For the RBS integration we used the <a href="K1497009" title="Opens internal link in current window" class="internal-link">K1497009</a> as template for a PCR with the following <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Primers" title="Opens internal link in current window" class="internal-link">primers</a>:</span></p> |
<p style="text-align:justify; line-height:150%"><span style="font-family:"Arial","sans-serif"" lang="EN-US"> F3H_RBS_Prefix and F3H_Suffix.</span></p></div></div><!--TYPO3SEARCH_end--> | <p style="text-align:justify; line-height:150%"><span style="font-family:"Arial","sans-serif"" lang="EN-US"> F3H_RBS_Prefix and F3H_Suffix.</span></p></div></div><!--TYPO3SEARCH_end--> | ||
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Revision as of 21:46, 17 October 2014
This part encodes the sequence for the enzyme Flavonon-3beta-hydroxylase (F3H; EC 1.2.7.3) from Petroselinum crispum with a strong ribosome binding site. To get a functional enzyme, we integrated a strong ribosomal binding site (RBS; B0034). For the RBS integration we used the K1497009 as template for a PCR with the following primers:
F3H_RBS_Prefix and F3H_Suffix.
