We build parts for decolor-assistant proteins(ManA1, ArfB, XynB) to assemble decolor-assistant machines. Compared to existing decolor-assistant proteins, our proteins can work at high temperature and have synergistic effects, which may be useful in paper industry.

ManA1, ArfB, XynB are three genes each encoding an enzyme. With help of these proteins, the pulp(which then become paper) can be bleached by only little chemical reagent. Since using less chemical reagent(especially the chlorinated) can help protect the environment, nowadays enzyme-assisted bleaching method is getting popular in paper making industry.

In our project, we build parts for three decolor-assistant proteins(ManA1, ArfB, XynB) to assemble three decolor-assistant machines. Through experiments, we confirm their decolor-assistant function, and we observe their heat-resistant property and synergistic effect.

To better understand the bio-assistant trend in paper industry, we also talk to local paper factory (we asked for a visit, but they all refused us. Some said this is the secret, and other suggested they are under emission standard). At the same time, we tightly communicate with several iGEM teams to improve our idea and appreciate their work. This is our first time to join the iGEM competition, and we hope we can have fun and get inspired in the following months.