Team:Tongji/lab-archives

From 2014.igem.org

iGEM Tongji 2014

Lab Archives

Here you can find our essential protocols. Please click "Download" buttons.

Protocol of PCR

PCR of XynB, ArfB and ManA1.

Protocol of Bacterium Cultivation

Cultivation of Clostridium stercorarium.

Here you can find our essential notebooks. Please click "Download" buttons.

Vector Construction

Report for expression vector construction

Expression Validation

Report for protein expression in E.coli

Function Validation

Report for DNS reaction by xynB

Hot-resistance Survey

Report for DNS reaction by xynB in high temperature.

Bleaching Modeling

Report for measuring xynB's bleaching effect

We prepared two versions of our parts for you. One is iGEM standard parts, with the official vector. They are universal but may not be so efficient. The other one is our modified parts, with the pET vector and can perfectly work with BL21(DE3) E.coli in our wetlab experiment. You can order from iGEM Registry for our iGEM standard parts or directly order from us for modified parts.

pET22b(+)_x1 Map

a pET22b(+) vector harboring XynB (without C-terminal His-tag)

pET22b(+)_x2 Map

a pET22b(+) vector harboring XynB (with C-terminal His-tag)

pET22b(+)_ArfB Map

a pET22b(+) vector harboring ArfB (without C-terminal His-tag)

pET22b(+)_ManA1 Map

a pET22b(+) vector harboring ManA1 (without C-terminal His-tag)

pSB1C3_XynB Map

a pSB1C3 vector harboring XynB

pSB1C3_ArfB Map

a pSB1C3 vector harboring XynB

pSB1C3_ManA1 Map

a pSB1C3 vector harboring XynB

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