Team:KIT-Kyoto/Notebook/Labnote

From 2014.igem.org

(Difference between revisions)
Line 38: Line 38:
</div>
</div>
<!--サイドメニュー終わり-->
<!--サイドメニュー終わり-->
-
 
<!--メインコンテンツ-->
<!--メインコンテンツ-->
<div id="container">
<div id="container">
Line 45: Line 44:
<h2>June</h2>
<h2>June</h2>
<div class="scroll"></div>
<div class="scroll"></div>
-
<a href=""><img src="https://static.igem.org/mediawiki/2014/7/7f/Kitlogo.png" width="120" height="120" alt="kit" style="float:right;margin:20px;"></a>
+
<!-- タイトル -->
-
<h3>6/24</h3>
+
<h3>June 10, 2014</h3>
-
<p class="sentence">KIT, Kyoto Institute of Technology, is a national university in Kyoto, which had been the capital city of Japan for more than a thousand years. KIT was founded to do research on silk and textile, important for artistic kimono industries for Kyoto.
+
<!-- タイトル終わり -->
-
+
 
-
Though its name has 'technology' in it, KIT is a fusion of science and arts. KIT has been committed in the genetics more recently. A good example is the Drosophila Genetic Resource Center (DGRC), which collects stocks of Drosophila, an essential model organism in life science research, from laboratories around the world. There are only two other such centers in the world.
+
<!-- 本文開始 -->
-
The institute has also collaborated actively with industries in Kyoto and beyond.
+
<p class="sentence">
-
+
<!-- 実験者名 -->
-
KIT's competence is beyond science. It also has arts and design courses.
+
OKAMOTO<br>
-
+
<!-- 実験者名終わり -->
-
KIT is a place of robust science and creative arts.  We will exert our strength
+
<!--Miniprep-->
-
As students of KIT, we are very proud of competing with wonderful students in other universities in iGEM. We will show you what you will get when arts and science merge. See you in Boston!
+
<strong>Miniprep</strong>
 +
<table class="materials">
 +
<tr>
 +
<th rowspan="2">Host</th>
 +
<th colspan="2">Sample</th>
 +
</tr>
 +
<tr>
 +
<th>Vector</th>
 +
<th>Insert</th>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</TD>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<tr>
 +
<td>BL21(DE3)</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS62</td>
 +
</tr>
 +
</table>
 +
<!--Miniprep終わり-->
 +
 
 +
<br>
 +
 
 +
<!--Restriction Digest-->
 +
<strong>Restriction Digest</strong>
 +
<table class="materials">
 +
<tr>
 +
<th rowspan="2">Host</th>
 +
<th colspan="2">Sample</th>
 +
<th rowspan="2">Enzyme</th>
 +
<th rowspan="2">Buffer</th>
 +
</tr>
 +
<tr>
 +
<th>Vector</th>
 +
<th>Insert</th>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE62</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE62</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
</table>
 +
<!--Ristriction Digest-->
 +
 
 +
<br>
</p>
</p>
-
<h2>July</h2>
+
<!--本文終わり-->
-
<div class="scroll"></div>
+
 
-
<h3>7/1</h3>
+
<!-- タイトル -->
 +
<h3>June 10, 2014</h3>
 +
<!-- タイトル終わり -->
 +
 
 +
<!-- 本文開始 -->
<p class="sentence">
<p class="sentence">
-
<div class="btn03">
+
<!-- 実験者名 -->
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
OKAMOTO<br>
-
</div>
+
<!-- 実験者名終わり -->
-
<div class="btn04">
+
<!--Miniprep-->
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
<strong>Miniprep</strong>
-
</div>
+
<table class="materials">
-
<div class="clear"><hr /></div>  
+
<tr>
-
<div class="btn03">
+
<th rowspan="2">Host</th>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
<th colspan="2">Sample</th>
-
</div>
+
</tr>
-
<div class="btn04">
+
<tr>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
<th>Vector</th>
-
</div>
+
<th>Insert</th>
-
<div class="clear"><hr /></div>
+
</tr>
-
<h3>7/4</h3>  
+
<tr>
-
<div class="btn03">
+
<td>BL21(DE3)</td>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
<td>pGEX6P-2</td>
-
</div>
+
<td>CuMTSE1</td>
-
<div class="btn04">
+
</tr>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
<tr>
-
</div>
+
<td>BL21(DE3)</td>
-
<div class="clear"><hr /></div>  
+
<td>pGEX6P-2</td>
-
<div class="btn03">
+
<td>CuMTSE2</TD>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
</tr>
-
</div>
+
<tr>
-
<div class="btn04">
+
<td>BL21(DE3)</td>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
<td>pGEX6P-2</td>
-
</div>
+
<td>CuMTS3</td>
-
<div class="clear"><hr /></div>  
+
<tr>
-
<div class="btn03">
+
<td>BL21(DE3)</td>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
<td>pGEX6P-2</td>
-
</div>
+
<td>CuMTS62</td>
-
<div class="btn04">
+
</tr>
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
</table>
-
</div>
+
<!--Miniprep終わり-->
-
<div class="clear"><hr /></div>  
+
 
-
<div class="btn03">
+
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
-
</div>
+
-
<div class="btn04">
+
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
-
</div>
+
-
<div class="clear"><hr /></div>  
+
-
<div class="btn03">
+
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
-
</div>
+
-
<div class="btn04">
+
-
<span class="before">Shoma Kokuryo | Applied Biology</span><span class="after">I'm tired</span>
+
-
</div>
+
-
<div class="clear"><hr /></div>
+
<br>
<br>
 +
 +
<!--Restriction Digest-->
 +
<strong>Restriction Digest</strong>
 +
<table class="materials">
 +
<tr>
 +
<th rowspan="2">Host</th>
 +
<th colspan="2">Sample</th>
 +
<th rowspan="2">Enzyme</th>
 +
<th rowspan="2">Buffer</th>
 +
</tr>
 +
<tr>
 +
<th>Vector</th>
 +
<th>Insert</th>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE62</td>
 +
<td>EcoR1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE1</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE2</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTS3</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
<tr>
 +
<td>BL21(DE3)PlysS</td>
 +
<td>pGEX6P-2</td>
 +
<td>CuMTSE62</td>
 +
<td>Sal1, Not1</td>
 +
<td>Buffer H</td>
 +
</tr>
 +
</table>
 +
<!--Ristriction Digest-->
 +
 +
<br>
 +
</p>
 +
<!--本文終わり-->
 +
 +
 +
 +
<h2>July</h2>
 +
<div class="scroll"></div>
<h3>7/25</h3>
<h3>7/25</h3>
<br>
<br>

Revision as of 06:24, 2 October 2014

notebook

LabNote

June

June 10, 2014

OKAMOTO
Miniprep

Host Sample
Vector Insert
BL21(DE3) pGEX6P-2 CuMTSE1
BL21(DE3) pGEX6P-2 CuMTSE2
BL21(DE3) pGEX6P-2 CuMTS3
BL21(DE3) pGEX6P-2 CuMTS62

Restriction Digest
Host Sample Enzyme Buffer
Vector Insert
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 Sal1, Not1 Buffer H

June 10, 2014

OKAMOTO
Miniprep

Host Sample
Vector Insert
BL21(DE3) pGEX6P-2 CuMTSE1
BL21(DE3) pGEX6P-2 CuMTSE2
BL21(DE3) pGEX6P-2 CuMTS3
BL21(DE3) pGEX6P-2 CuMTS62

Restriction Digest
Host Sample Enzyme Buffer
Vector Insert
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 Sal1, Not1 Buffer H

July

7/25



a
a
a
a
a




a
a
a
a
a




a
a
a
a
a

adfsfhao fhkna iehfalo difnzkl ncziehz odvnlkz dvaaa aaaaaaa aaa aaaaaa aaaaa aaaaakkkk kksks kksksk ksksk ksk ksks ksk ksksks kksksk skskksk s ksk skskksksksks kksksk ksk skks kks kskksksk

August




a
a
a
a
a



a
a
a
a
a




a
a
a
a
a




a
a
a
a
a




a
a
a
a
a



September




a
a
a
a
a



a
a
a
a
a




a
a
a
a
a




a
a
a
a
a




a
a
a
a
a



October




a
a
a
a
a



a
a
a
a
a




a
a
a
a
a




a
a
a
a
a




a
a
a
a
a



Top

Retrieved from "http://2014.igem.org/Team:KIT-Kyoto/Notebook/Labnote"