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Team:TU Darmstadt/Notebook/Labjournal/K1497012
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<!--TYPO3SEARCH_begin--><div id="c144" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Flavonon-3beta-hydroxylase with strong RBS (</span>B0034-F3H)</h1></div><div class="csc-textpic-text"><p style="text-align:justify; line-height:150%"><span style="font-family:"Arial","sans-serif"" lang="EN-US">This part encodes the sequence for the enzyme Flavonon-3beta-hydroxylase (F3H; EC 1.2.7.3) from <i>Petroselinum crispum </i>with a strong ribosome binding site. To get a functional enzyme, we integrated a strong ribosomal binding site (RBS; B0034). For the RBS integration we used the <a href="K1497009" title="Opens internal link in current window" class="internal-link">K1497009</a> as template for a PCR with the following <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Primers" title="Opens internal link in current window" class="internal-link">primers</a>:</span></p> | <!--TYPO3SEARCH_begin--><div id="c144" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">Flavonon-3beta-hydroxylase with strong RBS (</span>B0034-F3H)</h1></div><div class="csc-textpic-text"><p style="text-align:justify; line-height:150%"><span style="font-family:"Arial","sans-serif"" lang="EN-US">This part encodes the sequence for the enzyme Flavonon-3beta-hydroxylase (F3H; EC 1.2.7.3) from <i>Petroselinum crispum </i>with a strong ribosome binding site. To get a functional enzyme, we integrated a strong ribosomal binding site (RBS; B0034). For the RBS integration we used the <a href="K1497009" title="Opens internal link in current window" class="internal-link">K1497009</a> as template for a PCR with the following <a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials/Primers" title="Opens internal link in current window" class="internal-link">primers</a>:</span></p> | ||
| - | <p style="text-align:justify; line-height:150%"><span style="font-family:"Arial","sans-serif"" lang="EN-US"> F3H_RBS_Prefix and F3H_Suffix.</span></p></div></div><!--TYPO3SEARCH_end--> | + | <p style="text-align:justify; line-height:150%"><span style="font-family:"Arial","sans-serif"" lang="EN-US"> F3H_RBS_Prefix and F3H_Suffix.</span><br><br>With gelelectrophoresis and sequencing we verified that we produced the BioBrick <a href="http://parts.igem.org/Part:BBa_K1497012" title="Opens internal link in current window" class="internal-link"> BBa_K1497012</a></p></div></div><!--TYPO3SEARCH_end--> |
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Revision as of 23:13, 17 October 2014
Flavonon-3beta-hydroxylase with strong RBS (B0034-F3H)
This part encodes the sequence for the enzyme Flavonon-3beta-hydroxylase (F3H; EC 1.2.7.3) from Petroselinum crispum with a strong ribosome binding site. To get a functional enzyme, we integrated a strong ribosomal binding site (RBS; B0034). For the RBS integration we used the K1497009 as template for a PCR with the following primers:
F3H_RBS_Prefix and F3H_Suffix.
With gelelectrophoresis and sequencing we verified that we produced the BioBrick BBa_K1497012
