Team:LZU-China/NotebookB

From 2014.igem.org

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<span class="li"><a href="https://2014.igem.org/Team:LZU-China/Attribution">Attribution</a></span>
<span class="li"><a href="https://2014.igem.org/Team:LZU-China/Attribution">Attribution</a></span>
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<span class="li"><a href="https://2014.igem.org/Team:LZU-China/acknowledgement">Acknowledgement</a></span>
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<span class="li"><a href="https://2014.igem.org/Team:LZU-China/acknowledgement">Acknowledgements</a></span>
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<span class="li"><a href="">Team Profile</a></span>
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<span class="li"><a href="https://igem.org/Team.cgi?year=2014">Team Profile</a></span>
<span class="li"><a href="http://en.lzu.edu.cn/">Lanzhou University</a></span>
<span class="li"><a href="http://en.lzu.edu.cn/">Lanzhou University</a></span>
<span class="li"><a href="https://2013.igem.org/Team:LZU-China">LZU-CHINA 2013</a></span>
<span class="li"><a href="https://2013.igem.org/Team:LZU-China">LZU-CHINA 2013</a></span>

Revision as of 21:12, 17 October 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> LZU-China 2014

 
 
 

 

 

        "Rome was not built in a day." No matter which group we belong to, we took our work for almost a whole year. What did we do on earth? This notebook will show you in detail.

 

 

 

  

 

 

 
 
 

 

 

 

        THE NOTEBOOK OF WET LAB GROUP
 

     March 2014
Buy 300ml each chamber big MFC, buy multimeter, stainless steel, enameled wire. Buy PEM(proton exchange membrane) membrane and use H2O2 and H2SO4 boiled solution handle the PEM.

first appliance, and it is the biggest one. We use it and incubate a PNP endurance bacteria named LZU-3.We hoped that LZU-3 could degrade potassium dichromate, but we failed.

 

 

 April/June 2014
We prepared anode medium and cathode medium, incubate the Pseudomonas aeruginosa and Shewanella oneidensis and LZU-3. Testing the big MFC devices and collected the data.

 

 

July 2014
We tried to research how dose PNP influence the MFC power output. We buy 4 others MFC devices from internet, and prepare PEM carbon brush for it.

 

 

        

 

 

August 2014
We designed our own device and tried our best to manufacture the new MFC. We tested the performance of these 11 MFC devices. Different sizes(from 160*160*80 mm to 25*25*25 mm) were used in our experiment. At last we found that 50*50*50 mm is the best size of this battery.

      

  

 

 

 

  In September and October, we run our MFCs and gain a lot of data, we tried to figure out, dose riboflavin can stimulate the MFC power output, and, how accuracy did the genetically engineered E.coli response to the PNP concentration. we worked out empirical curves about the relationship between PNP concentration and output voltage.

 

 

We also measured the riboflavin expression data of the genatically modified E.coli.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

  

 

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