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Team:TU Darmstadt/Notebook/Labjournal/K1497012
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Revision as of 15:05, 17 October 2014
This part encodes the sequence for the enzyme Flavonon-3beta-hydroxylase (F3H; EC 1.2.7.3) from Petroselinum crispum with a strong ribosome binding site. To get a functional enzyme, we integrated a strong ribosomal binding site (RBS; B0034). For the RBS integration we used the K1497009 as template for a PCR with the following primers:
F3H_RBS_Prefix and F3H_Suffix.
