Team:KIT-Kyoto/Notebook/Labnote

From 2014.igem.org

(Difference between revisions)
Line 165: Line 165:
<!-- タイトル -->
<!-- タイトル -->
-
<h3>June 10, 2014</h3>
+
<h3>June 11, 2014</h3>
<!-- タイトル終わり -->
<!-- タイトル終わり -->
Line 171: Line 171:
<p class="sentence">
<p class="sentence">
<!-- 実験者名 -->
<!-- 実験者名 -->
-
OKAMOTO<br>
+
KOBAYASHI[[Media:Example.ogg]]<br>
<!-- 実験者名終わり -->
<!-- 実験者名終わり -->
-
<!--Miniprep-->
+
<!--AGE-->
-
<strong>Miniprep</strong>
+
<strong>AGE</strong>
<table class="materials">
<table class="materials">
<tr>
<tr>
-
<th rowspan="2">Host</th>
+
<th rowspan="2">Lane</th>
-
<th colspan="2">Sample</th>
+
<th colspan="2">Sample/Marker</th>
 +
<th rowspan="2">Enzyme</th>
</tr>
</tr>
<tr>
<tr>
Line 185: Line 186:
</tr>
</tr>
<tr>
<tr>
-
<td>BL21(DE3)</td>
+
<td>1</td>
 +
<td colspan="3">λDNA-HindⅢ</td>
 +
</tr>
 +
<tr>
 +
<td>2</td>
<td>pGEX6P-2</td>
<td>pGEX6P-2</td>
<td>CuMTSE1</td>
<td>CuMTSE1</td>
 +
<td>N.D</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BL21(DE3)</td>
+
<td>3</td>
<td>pGEX6P-2</td>
<td>pGEX6P-2</td>
-
<td>CuMTSE2</TD>
+
<td>CuMTS1</td>
 +
<td>EcoR1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BL21(DE3)</td>
+
<td>4</td>
<td>pGEX6P-2</td>
<td>pGEX6P-2</td>
-
<td>CuMTS3</td>
+
<td>CuMTSE1</td>
 +
<td>Sall,Not1</td>
 +
</tr>
<tr>
<tr>
-
<td>BL21(DE3)</td>
+
<td>5</td>
<td>pGEX6P-2</td>
<td>pGEX6P-2</td>
-
<td>CuMTS62</td>
+
<td>CuMTSE2</td>
 +
<td>N.D</td>
</tr>
</tr>
-
</table>
 
-
<!--Miniprep終わり-->
 
-
 
-
<br>
 
-
 
-
<!--Restriction Digest-->
 
-
<strong>Restriction Digest</strong>
 
-
<table class="materials">
 
<tr>
<tr>
-
<th rowspan="2">Host</th>
+
<td>6</td>
-
<th colspan="2">Sample</th>
+
-
<th rowspan="2">Enzyme</th>
+
-
<th rowspan="2">Buffer</th>
+
-
</tr>
+
-
<tr>
+
-
<th>Vector</th>
+
-
<th>Insert</th>
+
-
</tr>
+
-
<tr>
+
-
<td>BL21(DE3)PlysS</td>
+
<td>pGEX6P-2</td>
<td>pGEX6P-2</td>
-
<td>CuMTSE1</td>
+
<td>CuMTSE2</td>
<td>EcoR1</td>
<td>EcoR1</td>
-
<td>Buffer H</td>
 
</tr>
</tr>
<tr>
<tr>
-
<td>BL21(DE3)PlysS</td>
+
<td>7</td>
<td>pGEX6P-2</td>
<td>pGEX6P-2</td>
<td>CuMTSE2</td>
<td>CuMTSE2</td>
-
<td>EcoR1</td>
+
<td>Sal1, Not1</td>
-
<td>Buffer H</td>
+
</tr>
</tr>
<tr>
<tr>
-
<td>BL21(DE3)PlysS</td>
+
<td>8</td>
<td>pGEX6P-2</td>
<td>pGEX6P-2</td>
<td>CuMTS3</td>
<td>CuMTS3</td>
-
<td>EcoR1</td>
+
<td>N.D</td>
-
<td>Buffer H</td>
+
</tr>
</tr>
<tr>
<tr>
-
<td>BL21(DE3)PlysS</td>
+
<td>9</td>
<td>pGEX6P-2</td>
<td>pGEX6P-2</td>
-
<td>CuMTSE62</td>
+
<td>CuMTS3</td>
<td>EcoR1</td>
<td>EcoR1</td>
-
<td>Buffer H</td>
 
</tr>
</tr>
<tr>
<tr>
-
<td>BL21(DE3)PlysS</td>
+
<td>10</td>
<td>pGEX6P-2</td>
<td>pGEX6P-2</td>
-
<td>CuMTSE1</td>
+
<td>CuMTS62</td>
-
<td>Sal1, Not1</td>
+
<td>N.D</td>
-
<td>Buffer H</td>
+
</tr>
</tr>
<tr>
<tr>
-
<td>BL21(DE3)PlysS</td>
+
<td>11</td>
<td>pGEX6P-2</td>
<td>pGEX6P-2</td>
-
<td>CuMTSE2</td>
+
<td>CuMTS62</td>
-
<td>Sal1, Not1</td>
+
<td>EcoR1</td>
-
<td>Buffer H</td>
+
</tr>
</tr>
<tr>
<tr>
-
<td>BL21(DE3)PlysS</td>
+
<td>12</td>
<td>pGEX6P-2</td>
<td>pGEX6P-2</td>
-
<td>CuMTS3</td>
+
<td>CuMTS62</td>
-
<td>Sal1, Not1</td>
+
<td>Sall,Not1</td>
-
<td>Buffer H</td>
+
-
</tr>
+
-
<tr>
+
-
<td>BL21(DE3)PlysS</td>
+
-
<td>pGEX6P-2</td>
+
-
<td>CuMTSE62</td>
+
-
<td>Sal1, Not1</td>
+
-
<td>Buffer H</td>
+
</tr>
</tr>
 +
</table>
</table>
-
<!--Ristriction Digest-->
+
<!--AGE-->
<br>
<br>

Revision as of 06:48, 2 October 2014

LabNote

June

June 10, 2014

OKAMOTO
Miniprep

Host Sample
Vector Insert
BL21(DE3) pGEX6P-2 CuMTSE1
BL21(DE3) pGEX6P-2 CuMTSE2
BL21(DE3) pGEX6P-2 CuMTS3
BL21(DE3) pGEX6P-2 CuMTS62

Restriction Digest
Host Sample Enzyme Buffer
Vector Insert
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 EcoR1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE1 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE2 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTS3 Sal1, Not1 Buffer H
BL21(DE3)PlysS pGEX6P-2 CuMTSE62 Sal1, Not1 Buffer H

June 11, 2014

KOBAYASHI[[Media:Example.ogg]]
AGE

Lane Sample/Marker Enzyme
Vector Insert
1 λDNA-HindⅢ
2 pGEX6P-2 CuMTSE1 N.D
3 pGEX6P-2 CuMTS1 EcoR1
4 pGEX6P-2 CuMTSE1 Sall,Not1
5 pGEX6P-2 CuMTSE2 N.D
6 pGEX6P-2 CuMTSE2 EcoR1
7 pGEX6P-2 CuMTSE2 Sal1, Not1
8 pGEX6P-2 CuMTS3 N.D
9 pGEX6P-2 CuMTS3 EcoR1
10 pGEX6P-2 CuMTS62 N.D
11 pGEX6P-2 CuMTS62 EcoR1
12 pGEX6P-2 CuMTS62 Sall,Not1

July

7/25



a
a
a
a
a




a
a
a
a
a




a
a
a
a
a

adfsfhao fhkna iehfalo difnzkl ncziehz odvnlkz dvaaa aaaaaaa aaa aaaaaa aaaaa aaaaakkkk kksks kksksk ksksk ksk ksks ksk ksksks kksksk skskksk s ksk skskksksksks kksksk ksk skks kks kskksksk

August




a
a
a
a
a



a
a
a
a
a




a
a
a
a
a




a
a
a
a
a




a
a
a
a
a



September




a
a
a
a
a



a
a
a
a
a




a
a
a
a
a




a
a
a
a
a




a
a
a
a
a



October




a
a
a
a
a



a
a
a
a
a




a
a
a
a
a




a
a
a
a
a




a
a
a
a
a



Top

Retrieved from "http://2014.igem.org/Team:KIT-Kyoto/Notebook/Labnote"