Team:LZU-China

From 2014.igem.org

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<title>LZU-China 2014</title>
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<h1 >WELCOME TO LZU-iGEM 2014! </h1>
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<p>Our brilliant team named LZU-China are ready to start our own iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:LZU-China&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:LZU-China"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:LZU-China/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=LZU-China"style="color:#000000"> Official Team Profile </a></td>
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<li><a href="https://2014.igem.org/Team:LZU-China/Modeling">Modeling</a> </li>
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<li><a href="https://2014.igem.org/Team:LZU-China/Notebook">Notebook</a> </li>
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<li><a href="https://2014.igem.org/Team:LZU-China/Safety">Safety</a> </li>
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<p>There are a few wiki requirements teams must follow:</p>
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
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<li>All pages must be created under the team’s name space.</li>
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<li>As part of your documentation, keep the links from the menu to the left. </li>
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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  <p>&nbsp;</p>
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  <p>&nbsp;</p>
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  <p>&nbsp;</p>
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</div>
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<tr>
 
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<td width="45%" valign="top">
 
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<p>We are currently working on providing teams with some easy to use design templates.
 
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
 
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<ul>
 
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
 
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
 
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
 
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
 
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
 
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</ul>
 
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a> lists. </p>
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<div id="apDiv3">
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</td>
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  <p>&nbsp;</p>
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  <p>&nbsp;</p>
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  <p>&nbsp;</p>
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  <div>CONTENTS</div>
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  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
  <table width="100%" border="0" align="center">
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    <tr>
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      <td width="20%"><p>&nbsp;</p>
 +
      <p>&nbsp;</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/background"><img src="https://static.igem.org/mediawiki/2014/8/83/1backgroud.png" width="100" height="100" /></a></p>
 +
      <p>Background</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/wetlab"><img src="https://static.igem.org/mediawiki/2014/d/d5/2wetlab.png" width="100" height="100" /></a></p>
 +
      <p>Wet Lab</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/drylab"><img src="https://static.igem.org/mediawiki/2014/c/c6/3drylab.png" width="100" height="100" /></a></p>
 +
      <p>Dry Lab</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/Parts"><img src="https://static.igem.org/mediawiki/2014/1/14/4parts.png" width="100" height="100" /></a></p>
 +
      <p>Parts</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/hp"><img src="https://static.igem.org/mediawiki/2014/5/5f/5humanpractics.png" width="100" height="100" /></a></p>
 +
      <p>Human Practice</p></td>
 +
      <td width="20%">&nbsp;</td>
 +
    </tr>
 +
    <tr>
 +
      <td width="20%">&nbsp;</td>
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      <td width="12%"><p>&nbsp;</p>
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      <p>&nbsp;</p>
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      <p>&nbsp;</p></td>
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      <td width="12%">&nbsp;</td>
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      <td width="12%">&nbsp;</td>
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      <td width="12%"><p>&nbsp;</p></td>
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      <td width="12%">&nbsp;</td>
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      <td width="20%">&nbsp;</td>
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    </tr>
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    <tr>
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      <td width="20%">&nbsp;</td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/Team"><img src="https://static.igem.org/mediawiki/2014/a/a2/6members.png" width="100" height="100" /></a></p>
 +
      <p>Our team</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/interlab"><img src="https://static.igem.org/mediawiki/2014/c/cc/7interlab.png" width="100" height="100" /></a></p>
 +
      <p>Interlab</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/Safety"><img src="https://static.igem.org/mediawiki/2014/e/ee/8safety.png" width="100" height="100" /></a></p>
 +
      <p>Safety</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/Notebook"><img src="https://static.igem.org/mediawiki/2014/0/00/9notebook.png" width="100" height="100" /></a></p>
 +
      <p>Notebook</p></td>
 +
      <td width="12%"><p><a href="https://2014.igem.org/Team:LZU-China/future"><img src="https://static.igem.org/mediawiki/2014/0/04/10future.png" width="100" height="100" /></a></p>
 +
      <p>Future Work</p></td>
 +
      <td width="20%">&nbsp;</td>
 +
    </tr>
 +
  </table>
 +
  <p>&nbsp;</p>
 +
  <p>&nbsp;</p>
 +
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      <td><div> *In order to get better user experience, we recommend the Internet Explorer 10 (or higher version). And the <span id="tran_1_0" data-aligning="#src_1_0,#tran_1_0" jquery171013312911573948127="19">best</span> <span id="tran_1_1" data-aligning="#src_1_1,#tran_1_1" jquery171013312911573948127="16">resolution</span> is 1366 X 768.</div>
 +
        .</td>
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  </table>
 +
  <p>&nbsp;</p>
 +
</div>
 +
<div id="apDiv4" >
 +
  <p>&nbsp;</p>
 +
  <table width="100%" height="627" border="0">
 +
    <tr>
 +
      <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" height="623" valign="top"><p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <div> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;ABSTRACT</div>
 +
      <table width="100%" border="0">
 +
        <tr>
 +
          <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="30%">&nbsp;</td>
 +
          <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="57%">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs still have disadvantages such as hard to determine the contaminants concentrations which is a major drawback for MFCs applications. </p>
 +
            <p>&nbsp;</p>
 +
            <table width="100%" border="0">
 +
              <tr>
 +
                <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" ><img src="https://static.igem.org/mediawiki/2014/4/46/Bigabstract1.png" width="692" height="170" /></td>
 +
              </tr>
 +
            </table>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In our study, we found that the electricity produced by MFCs was weakly correlated to the substrates' concentrations such as P-nitrophenol (PNP) in anode or Chromium (VI) in cathode. But the electricity produced was not stable and so did the correlations. Therefore, we hypothesize that a) by using genetic engineered bacteria, the MFC's electricity will be more stable and correlated with substrates concentrations. b) By monitoring MFC's electricity, it will be possible to measure substrates' concentrations.</p>
 +
            <p>&nbsp;</p>
 +
            <table width="100%" border="0">
 +
              <tr>
 +
                <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" ><img src="https://static.igem.org/mediawiki/2014/5/5f/Bigabstract2.png" alt="" width="692" height="203" /></td>
 +
              </tr>
 +
            </table>
 +
            <p>&nbsp;</p>
 +
            <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In this study, we've designed a novel MFC system. For anode strain, we've cloned a NO3- sensor sequence and a riboflavin producing genes into Escherichia coli. The recombinants is able to detect PNP and produce riboflavin to boost electrical generation when co-cultured with Shewanella oneidensis. In the cathode, gene codes chromate (VI) reductase Yief was cloned into E.coli. The stability of MFCs has been improved and the electricity generated was correlated with the substrates. Moreover, based on this correlation, we've designed a cell phone program which is able to remotely monitor the contaminants concentrations in MFCs. </p>
 +
            <p><br />
 +
              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br />
 +
          </p></td>
 +
          <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="13%">&nbsp;</td>
 +
        </tr>
 +
      </table>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
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      <p>&nbsp;</p>
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      <p>&nbsp;</p>
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      <p>&nbsp;</p>
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      <p>&nbsp;</p>
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      <p>&nbsp;</p>
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      <p>&nbsp;</p>
 +
      <p>&nbsp;</p></td>
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<span class="li"><a href="http://en.lzu.edu.cn/">Lanzhou University</a></span>
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<span class="li"><a href="https://2013.igem.org/Team:LZU-China">LZU-CHINA 2013</a></span>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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Latest revision as of 21:45, 17 October 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> LZU-China 2014

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

CONTENTS

 

 

 

 

 

Background

Wet Lab

Dry Lab

Parts

Human Practice

  
 

 

 

 

    

 

    
 

Our team

Interlab

Safety

Notebook

Future Work

  

 

 

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        ABSTRACT
              

            Microbial Fuel Cells (MFCs) which can convert contaminants in wastewaters into energy is an ideal approach to solve both pollution problem and energy crisis. However, MFCs still have disadvantages such as hard to determine the contaminants concentrations which is a major drawback for MFCs applications.

 

 

            In our study, we found that the electricity produced by MFCs was weakly correlated to the substrates' concentrations such as P-nitrophenol (PNP) in anode or Chromium (VI) in cathode. But the electricity produced was not stable and so did the correlations. Therefore, we hypothesize that a) by using genetic engineered bacteria, the MFC's electricity will be more stable and correlated with substrates concentrations. b) By monitoring MFC's electricity, it will be possible to measure substrates' concentrations.

 

 

            In this study, we've designed a novel MFC system. For anode strain, we've cloned a NO3- sensor sequence and a riboflavin producing genes into Escherichia coli. The recombinants is able to detect PNP and produce riboflavin to boost electrical generation when co-cultured with Shewanella oneidensis. In the cathode, gene codes chromate (VI) reductase Yief was cloned into E.coli. The stability of MFCs has been improved and the electricity generated was correlated with the substrates. Moreover, based on this correlation, we've designed a cell phone program which is able to remotely monitor the contaminants concentrations in MFCs.


            

  

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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