Team:LZU-China/Parts
From 2014.igem.org
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<table width="100%" border="0"> | <table width="100%" border="0"> | ||
<tr> | <tr> | ||
- | <td width=" | + | <td width="5%" height="408"> </td> |
- | <td width=" | + | <td width="90%"><p> </p> |
<p> </p> | <p> </p> | ||
- | <table width=" | + | <table width="100%" border="2" align="center" cellpadding="0" cellspacing="0"> |
+ | <col width="72" /> | ||
+ | <col width="139" /> | ||
+ | <col width="96" /> | ||
+ | <col width="72" span="3" /> | ||
+ | <col width="244" /> | ||
+ | <col width="72" /> | ||
+ | <col width="333" /> | ||
<tr> | <tr> | ||
- | <td width=" | + | <td width="72">No</td> |
- | <td width=" | + | <td width="139">Part No.</td> |
- | <td width="72">< | + | <td width="96">NCBI No.</td> |
- | <td width=" | + | <td width="72">Resistance</td> |
- | <td width=" | + | <td width="72">Vector</td> |
- | <td width=" | + | <td width="72">Gene Name</td> |
+ | <td width="244">Gene function</td> | ||
+ | <td width="72">Length</td> | ||
+ | <td width="333">Website (NCBI)</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td | + | <td>1</td> |
- | <td | + | <td>BBa_K1523021</td> |
- | <td | + | <td>NC_004999.1</td> |
- | <td | + | <td>Chl</td> |
- | <td width=" | + | <td>pSB1C3</td> |
- | <td | + | <td>nahR</td> |
+ | <td width="244">nahE coding sequence, <br /> | ||
+ | encoding a LysR-type transcriptional regulator</td> | ||
+ | <td>903 bp</td> | ||
+ | <td><a href="http://www.ncbi.nlm.nih.gov/gene/1343208">http://www.ncbi.nlm.nih.gov/gene/1343208</a></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td | + | <td>2</td> |
- | <td width=" | + | <td>BBa_K1523022</td> |
- | <td | + | <td width="96">NC_007926.1<br /> |
- | <td | + | (37386..38381)</td> |
- | <td | + | <td>Chl</td> |
- | <td | + | <td>pSB1C4</td> |
+ | <td>nahE</td> | ||
+ | <td>nahE coding sequence</td> | ||
+ | <td>1005 bp</td> | ||
+ | <td><a href="http://www.ncbi.nlm.nih.gov/gene/3974214">http://www.ncbi.nlm.nih.gov/gene/3974214</a></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td | + | <td>3</td> |
- | <td width=" | + | <td>BBa_K1523023</td> |
- | <td | + | <td width="96">NC_007926.1<br /> |
- | <td | + | (44492..45796)</td> |
- | <td | + | <td>Chl</td> |
- | <td | + | <td>pSB1C5</td> |
+ | <td>nahG</td> | ||
+ | <td>nahG coding sequence</td> | ||
+ | <td>1305 bp</td> | ||
+ | <td><a href="http://www.ncbi.nlm.nih.gov/gene/3974216">http://www.ncbi.nlm.nih.gov/gene/3974216</a></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td | + | <td>4</td> |
- | <td | + | <td>BBa_K1523025</td> |
- | <td | + | <td>NC_017530.1</td> |
- | <td | + | <td>Chl</td> |
- | <td | + | <td>pSB1C6</td> |
- | <td | + | <td>chrR</td> |
+ | <td>chrR coding sequence</td> | ||
+ | <td>666 bp</td> | ||
+ | <td><a href="http://www.ncbi.nlm.nih.gov/gene/12519943">http://www.ncbi.nlm.nih.gov/gene/12519943</a></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td | + | <td>5</td> |
- | <td | + | <td>BBa_K1523101</td> |
- | <td | + | <td>N/A</td> |
- | <td | + | <td>Chl</td> |
- | <td | + | <td>pSB1C7</td> |
- | <td | + | <td>PyeaR</td> |
+ | <td>PyeaR->Riboflavin synthase</td> | ||
+ | <td>3726 bp</td> | ||
+ | <td>N/A</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>6</td> | ||
+ | <td>BBa_K1523008</td> | ||
+ | <td>NC_007779.1</td> | ||
+ | <td>Chl</td> | ||
+ | <td>pSB1C8</td> | ||
+ | <td>Yief</td> | ||
+ | <td>yieF coding sequence</td> | ||
+ | <td>560 bp</td> | ||
+ | <td><a href="http://www.ncbi.nlm.nih.gov/gene/12519943">http://www.ncbi.nlm.nih.gov/gene/12933177</a></td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
<p> </p> | <p> </p> | ||
+ | <p> </p></td> | ||
+ | <td width="5%"> </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <table width="100%" border="0"> | ||
+ | <tr> | ||
+ | <td> </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p><br /> | ||
+ | </p> | ||
<p> </p> | <p> </p> | ||
<p> </p> | <p> </p> | ||
- | + | <p> </p> | |
- | + | <table width="100%" border="0"> | |
- | <td | + | <tr> |
+ | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" height="65"> | ||
+ | <p> </p><p> </p> | ||
+ | <div> GEL PICTURES OF SOME PARTS</div></td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
- | |||
- | |||
<table width="100%" border="0"> | <table width="100%" border="0"> | ||
<tr> | <tr> | ||
- | <td width="22%" height="408"> </td> | + | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="22%" height="408"> </td> |
- | <td width="56%"><p> </p> | + | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="56%"><p> </p> |
<p> </p> | <p> </p> | ||
<p> </p> | <p> </p> | ||
<p><img src="https://static.igem.org/mediawiki/2014/c/c0/Partpic1.png" width="560" height="378" /></p> | <p><img src="https://static.igem.org/mediawiki/2014/c/c0/Partpic1.png" width="560" height="378" /></p> | ||
<p> </p> | <p> </p> | ||
- | <p><img src="https://static.igem.org/mediawiki/2014/e/e0/Partpic2.png" width=" | + | <p><img src="https://static.igem.org/mediawiki/2014/e/e0/Partpic2.png" width="451" height="419" /></p> |
<p>Fig.2 The picture of K1523101. 1,3: pSB1C3 with K1523101 cut by EcoRI; 2,4: pSB1C3 with K11523101 cut by EcoRI and HindIII; 5: pSB1C3 with K1523101 not be cut.</p> | <p>Fig.2 The picture of K1523101. 1,3: pSB1C3 with K1523101 cut by EcoRI; 2,4: pSB1C3 with K11523101 cut by EcoRI and HindIII; 5: pSB1C3 with K1523101 not be cut.</p> | ||
- | |||
- | |||
<p> </p> | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p></p> | ||
<p><br /> | <p><br /> | ||
</p></td> | </p></td> | ||
- | <td width="22%"> </td> | + | <td background="https://static.igem.org/mediawiki/2014/a/a6/Huils.jpg" width="22%"> </td> |
</tr> | </tr> | ||
</table> | </table> | ||
+ | <table width="100%" border="0"> | ||
+ | <tr> | ||
+ | <td height="48"><p> </p> | ||
+ | <p> </p> <div> PARTS SEQUENCES</div></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p>>nahR naphthalene degradation LysR-family transcriptional activator 903bp<br /> | ||
+ | ATGGAACTGCGTGATCTGGATTTAAACCTGCTGGTGGTGTTCAACGAGCTGCTGGTCTGCAGACGCGTCTCTATCGTTGCCGAGAACCTGGGCCTGACCCAGCCTGCTGTCAGCAATGCGCTGAAACGCCTGCGCACAGCGCTACAGGACCAACTTTTCGTGCGCACCTACCAGGGTATGGAACCCACACCCTACGCCGCGAACTTGGCCGAGCCCGTCGCCTTGGCCATGCACGCCCTGCGCGAAGCCCTGCAGCACCATGAGCGCTTCGATCCGCTGACCAGCGAGCGTACTTTCACCCTGGCCATGACCGACATCGGCGAGATCTACTTCATGCCGCGGCTGATGGATGCGATTACTCTTCAAGCCCCCAATTGCGCGATCAGCACCGTGCGCGACAGTTCGATGAGCCTGATGCAAGCCTTGCAGAACGGCACAGTGGACCTAGCCGTGGGCCTGCTGCCCAACCTGCAGACTGGCTTCTTCCAGCGCCGGCTGCTCCACAATCACTACGTGTGCCTGTGTCGCAAGGACCATCCGGCCACCCGCGAACCCCTGACTCTTGAGCGCTTCTGTTCCTATGGCCACGTGCGTGTCATCGCCGCTGGCACAGGCCACGGCGAGGTGGACACATACTTGACGAAGGCCGGCATCCGGCGCGACATCCGCCTGGAGGTGCCGCACTTCGTCGCCGTTGGCCACATACTCCAGCGCACCGAGCTGATCGCCACTGTGCCGATACGTTTCGCCGACTGCTGCGTAGAGCCCTTCGGTCTGAGCGTCTTGCCGCACCCAGTCGCCTTGCCGGAAATCGCCATCAACATGTTCTGGCATGCGAAATACCACCAGGACCTCGCCAATATCTGGTTGCGGCAACTGATGTTCGACCTGTTTTCTGATTGA</p> | ||
+ | <p>>nahE trans-o-hydroxybenzylidenepyruvate hydratase-aldolase 1005bp<br /> | ||
+ | ATGTCGAATAAAATTATGAAAACGTCGCGTCTTACCGCCGAAGATATCAACGGCGCCTGGACTATAATGCCCACACCCTCGACGCCTGATGCTTCTGATTGGCGCAGCACTGCCACCGTGGACTTAGAAGAGACTGCCCGCATAGTTGAAGAGCTGATTGCAGCTGGTGTCAACGGTATTCTAAGTATGGGTACTTTTGGTGAGTGCGCCACGTTGACCTGGGATGAAAAACGTGATTATGTCTCGACGATTGTCGAGACCATTCGTGGTCGCGTGCCTTATTTCTGTGGCACGACAGCCTTAAATACCCGAGAAGTCATCCGCCAGACCCGAGAGCTTATCGATATTGGCGCCAACGGCACTATGCTCGGGGTGCCGATGTGGGTGAAGATGGACCTGCCTACAGCGGTTCAGTTCTATCGTGATGTTGCAGATGCGGTACCAGAGGCTGCCATTGCGATTTACGCCAACCCCGAAGCATTCAAGTTCGACTTCCCTCGCCCATTCTGGGCAGAGATGTCCAAAATTCCGCAGGTAGTGACTGCGAAGTATCTAGGCATCGGAATGCTTGACTTGGACCTGAGACTGGCACCCAACATCCGCTTCCTTCCCCACGAAGATGACTATTACGCGGCCGCACGCATCAATCCCGAGCGCATAACCGCGTTCTGGTCAAGCGGGGCCATGTGCGGCCCGGCTACCGCCATCATGTTGCGTGACGAAGTGGTGCGGGCCAAGAGCACCGGTGACTGGGCCAAGGCCAAAGCCATCTCCGATGATATGCGTGCAGCCGACTCGACATTGTTTCCGCGTGGCGACTTTTCGGAGTTCTCGAAGTATAATATCGGGCTTGAAAAGGCACGGATGGATGCGGCTGGTTGGCTCAAGGCTGGGCCCTGCCGTCCGCCCTACAACCTTGTTCCAGAAGACTACCTCGCTGGTGCACAGAAATCAGGCAAGGCTTGGGCCGCGCTGCACGCTAAATACAGTAATGAATTGAAGTAG</p> | ||
+ | <p>>nahG salicylate hydroxylase 1305bp<br /> | ||
+ | ATGAAAAACAATAAACCTGGCTTGCGCATCGGTATCATCGGCGGCGGGATTTCCGGCGTTGCCTTGGCTCTGGAGCTCTGTCGCTACTCCCATCTCCAAGTACAGCTGTTCGAGTGCGCGCCGGCTTTTGGTGAGGTCGGTGCAGGAGTTTCCTTTGGCCCCAACGCGGTGCGCGCCATTGTCGGCCTAGGCTTGGGCGAGGCCTACTTGCAGGTCGCCGACCGTACCTCGGAGCCCTGGGAGGACGTGTGGTTCGAGTGGCGGCGCGGCAGGGATGCCAGCTATCTGGGAGCGACCATCGCTCCGGGCGTGGGCCAGTCCTCGGTACACCGGGCGGATTTCCTCGATGCCCTAGTAAATCACCTCCCAAAAGGTATCGCCCAATTCAGGAAGCGCGCCACACAGGTCGAGCAAAAGGGGGGCGAAGTGCAAGTGCTATTCGCCGACGGCACAGAGCACCGCTGTGACCTTCTAATCGGTGCCGACGGAATCAAGTCAGCGCTGCGTAGTCATGTGCTGGAAGGTCAGGGGCTGGCCCCACAGGTACCGCGCTTCAGCGGCACCTGTGCCTATCGGGGGATGGTCGACAGCCTACACCTGCGCGAAGCCTATCGAGCCCAGGGCATCGACGAGCACTTGGTGGACGTACCGCAGATGTACCTAGGGCTTGACGGCCATATCCTCACTTTTCCGGTGAGGAATGGCCGCCTCATCAACGTTGTGGCCTTCATTTCCGACCGTAGTGAGCCGAAGCCGAACTGGCCCGCGGATGCCCCTTGGGTGCGCGATGTGAGCCAGCGAGAGATGCTCGATGCCTTCGCGGGTTGGGGTGATGCCGCGCGCACCCTGCTGGAGTGCATCCCGGCACCAACTCTCTGGGCACTGCACGACCTGGCGGAGCTGCCGGGCTACGTGCACGGGCGGGTCGTCCTGATCGGCGACGCAGCTCACGCCATGCTGCCGCACCAAGGCGCCGGTGCCGCCCAAGGACTTGAGGACGCCTACTTCCTCGCCCGCCTGTTGGGCGATACCCAAGTCGATGCAGACAACCTCGCCGAGCTGCTTGAAGCCTACGACGACCTGCGCCGCCCTCGTGCCTGTCGCGTGCAGCGAACCTCCCGGGAGACCGGCGAGTTATACGAGTTTCGCGACCCCGTCGTAGGTGCGAACGAGCATCTGCTGGGGGAAAACCTGGCGACCCGCTTCGACTGGCTGTGGAGCCACGACCTCGACGCCGACCTGGCCGAGGCACGTGCGCGCCTGGGTTGGGAAAATGGTAGCCGGGGTGTGCTACGTCAAGGGTGA</p> | ||
+ | <p>>ChrR chromate reductas 666 bp<br /> | ||
+ | ATGAAAAAAATAGTCCAGTCGGAAGGTTTTCGGTTCTCCGCTGGTCCAATTGTTCGCCCGATCATCTATAAATGGGGCTCCCTTGAACCCATCGTCGAGAAGTGTCCAATGAGTAATGTCTACACCGTGGCCGTTTTAGTGGGGAGCCTGCGCAAGGCTTCGATCAATCGCAAGCTGGCTCTGGCCCTGGCGGATCTGGCGCCGAGCAACCTGAAGCTGGAAATCATCGAGATAGGCGACCTGCCGCTCTACAACGAAGATATTGACGTCACACCCCCACCAGCCTACAGCACTTTCCGTGAGCAGGTTGGCGCGGCCGACGCATTGCTGTTTGTCACGCCTGAATACAACCGCTCGGTTCCGGCCCCGATGAAAAATGCCATTGACGTCGGCTCGCGGCCTTACGGCAAGAGCTGCCTGAGCGGTAAACCGGGTGCGGTGATCAGTGCCTCGCCGGGAGCCATGGGTGGTTTCGGCGCCAATCATCATTTGCGCCAGTCCCTGGTTTTCCTCGATGTGCCTTGCATGCAACAGCCGGAGGCTTATCTGGCCGGTGCGGGGAGCGCGTTCGATGAGCAGGGCGAGCTGTCTGAGTCGGTGAGGCCGTTTCTGCAGAAATTCATTGATGCCTATGGCAAGTGGGTTGAACAGCACAAAAAGGCCTGA</p> | ||
+ | <p>>BBa_K1523101 Part-only sequence (3726 bp)<br /> | ||
+ | TTCCCATCTATAATCCTCCCTGATTCTTCGCTGATATGGTGCTAAAAAGTAACCAATAAATGGTATTTAAAATGCAAATTATCAGGCGTACCCTGAAACG<br /> | ||
+ | TACTAGAGATTAAAGAGGAGAAATACTAGATGAATTGGTCAGTACTCGATAACCAAATGATGAGCCGAGCTATTCAATTGGCTCGCAAAGGATTTTATAC<br /> | ||
+ | CACTCGCCCCAATCCCAGTGTGGGCTGCGTTATCGTTAACGATAACCAGATTGTCGGTGAAGGTTATCATCAAAAAGCCGGAGAGCCCCACGCTGAAGTG<br /> | ||
+ | CATGCGCTGCGCATGGCTGGCGAGCTTGCCCGTGGCGCGACTGCTTATGTCACCTTAGAACCCTGTAGCCATTATGGTCGTACGCCGCCCTGCGCCTTGG<br /> | ||
+ | CGCTGATCAATATTGGTGTAAAGCGGGTGGTGGTGGCGGTTGAAGATCCTAATCCGCAGGTCGGCGGGCGCGGTATTCAAATGCTGCGCGATGCGGGTAT<br /> | ||
+ | TCAAGTGGATGTGGGCTTGCATCGTGATGAGGCCTATGCCTTAAATCTTGGTTTTATGAAGCGCATGGAGTCGGGTCTACCTTGGGTGACGGTAAAGCTT<br /> | ||
+ | GCCGCGAGTCTTGATGGTAAAACTGCGTTATCAAACGGGGTGTCTAAATGGATCACTGGCCCTGAAGCTCGCCGCGATGTGCAGCGCTTAAGATTACGAT<br /> | ||
+ | CCTGCGCGCTGATCACGGGGATTGAAACGGTACTGGCCGATGATCCATCGCTCAATGTGCGCTACTCAGAGCTTGGCAGTTTAAGAGCGCAATTGAGTGA<br /> | ||
+ | GTCGCAGGTTTTACAGCCGCTGCGAGTGGTTTTGGACAGTCGCGCCCGCATGCCTATCACGGCAGCATTGTTTGCGATTGAATCACCCATTTTATTAGTC<br /> | ||
+ | TCGACAGTGAGCTATTCGTCAGACTTTATGGTGCAATTACCCGCCCATGTGACTTGCCTCCAATTACCAGCCATTGAGGGTCGAATCTCCTTGTCTGCAC<br /> | ||
+ | TATTAAGCTATTTAGGCAAAAGCTGTAATCAGGTGCTTATCGAAGCGGGCGCGACTTTAGCTGGCGCCTTTATCGGCTCAGGATTAGCCGATGAATTAGT<br /> | ||
+ | GCTTTATCAAGCGATGAAAATCCTTGGCGGCCAAGGACGTAATCTACTTGAATTACCCGATTATCAAATGATGGCCGATATTCCGACCCTCAAATTGGTC<br /> | ||
+ | GACGAGCGTAAAGTGGGCGCGGATACGCGCTTCACCTTGAGGCTCGAGTCCAATCCATCTTTAGCGAATAAGTGAGTTAACCATGTTTACTGGGATTATT<br /> | ||
+ | GAGGCCGTTGGTACACTGCGAAAGCTTGAGCGTAAAGGCGATGATATTCGCTTAACGGTCGCCAGTGGCAAACTGGATTTAACCGATGTGCGTTTAGGCG<br /> | ||
+ | ACAGTATCGCCACCAACGGTGTATGTTTGACCGTGGTTCAACAGTTAGCCGATGGCTATGTGGCTGATGTGTCAGCTGAAACCGTCAGCCTTACAGGTTT<br /> | ||
+ | TGCAAACTATAAAGTGGGTACTAAGGTCAATCTTGAAAAGGCCGTCACCCCAACTACTCGTTTAGGCGGCCATATGGTGAGTGGTCATGTGGATGGGATT<br /> | ||
+ | GCTACTGTTGAGCAGCGTCTTGCTCGTGGTCAAGCAATCGAATTTTGGTTAGCGGCTCCCACGGAGCTTGCTCGCTATATTGCCCATAAAGGTTCTATCA<br /> | ||
+ | CGATTGATGGCGTGAGCCTGACAGTAAACGAAGTCGATGGACACCGTTTTCGATTAACGATTGTGCCCCATACTGTAGGTGAAACCACGTTGGTGGATTT<br /> | ||
+ | AAAAGCAGGCGATAAGGTCAATATTGAAGTGGACTTAATTGCCCGTTATTTAGAGCGTTTAATGCGTTTTGACAGCAAAGAAACCCAAGGCGGAGGGGTG<br /> | ||
+ | ACCATGGAAATGTTAGCCCGTGCTGGCTTTGTGCGTTAGTACACTGGCACTCAGTTCACTTAGGTATAGAATTTATAACAACAGTCACATTATAAAGGTC<br /> | ||
+ | CTACAATGGCGCTGCACAGTATAGAAGAGATCATCGAGGATATTCGTCAAGGCAAAATGGTTATTTTGATGGATGACGAGGACAGAGAAAACGAAGGCGA<br /> | ||
+ | TCTGATTTTGGCGGCCGAAATGGTGACGCCAGAAGCGATTAACTTTATGGCGAAATACGGCCGCGGGTTAATCTGCCAAACGATGACCAAGGCCCGTTGC<br /> | ||
+ | CAGCAGTTAAATCTGCCCTTGATGGTAACCAATAACAATGCGCAGTTCTCGACCAACTTTACGGTATCGATTGAAGCTGCCGAAGGCGTAACCACAGGGA<br /> | ||
+ | TTTCGGCCCACGATCGCGCTGTAACCGTTAAAGCGGCCGTAGCAAAAGATGCTAAAGCGTCGGACTTAGTTCAGCCTGGGCATATCTTCCCGTTAATGGC<br /> | ||
+ | GCAGGATGGTGGCGTATTAACCCGCGCAGGTCACACTGAAGCGGGCTGTGATTTAGCCCGTTTAGCAGGGCTTGAACCATCGGGGGTGATTGTTGAAATT<br /> | ||
+ | TTGAACGAAGACGGCACTATGGCCCGTCGCCCAGATTTAGAGATTTTCTCTGAAAAACATGGCATTAAAATCGGCACTATTGCGGCCCTGATTGAATACC<br /> | ||
+ | GTAATACCAAAGAAACGACCGTTGTGCGTGAAGCCAAATGCAAACTGCCAACCCGTTTCGGTGAGTTCGATATGGTGACCTTTAGGGATACTATCGACAA<br /> | ||
+ | TCAATTGCACTTTGCCTTAGTGAAAGGCGAGGTGAAGCCTGACTGTTTAGTACGGGTACATTTACAGAATACCTTTAACGATCTACTTCACTCTGAGCGT<br /> | ||
+ | GATCAGCAACGTAGCTGGCCGCTCGAAAAAGCGATGGCGCGGATTTCTGCCGAAGGTGGTGTATTAGTACTGCTTGGCAATCAGGAACATTCCAGTGAAA<br /> | ||
+ | TCCTCGCCAAAGTCAAAGCCTTTGAAGCGGAAGATCAAGGTCAGACCCCAGTATCGGCTAAATGGCAAGGCACCTCACGCCGTGTAGGTGTGGGTTCGCA<br /> | ||
+ | AATCCTCGCCAGTTTAGGCGTGACAAAAATGCGACTCTTAAGCTCGCCCAAACGTTATCACTCACTTTCAGGCTTTGGCCTTGAAGTGACCGAATGCGTA<br /> | ||
+ | GCTGAGTAACAGGTTAAGCTTGTAATAGGTTGCACTAAGGTAAAATTAACCATTTCTATCATTTGCATAAGGCCGCGGGCAATTGACTGTGGTATCATGT<br /> | ||
+ | CGCCACTTTTCGCCCAGAGCTGGGTGCTTTAGCTAAATTAGGTAAGAAAATGAACGTAGTTCAAGGTAATATCGAAGCGAAGAATGCCAAAGTTGCGATT<br /> | ||
+ | GTAATTTCGCGTTTTAACAGCTTTTTAGTCGAAAGCCTGCTTGAAGGTGCACTTGACACGCTGAAACGTTTTGGCCAAGTCAGTGATGAAAACATCACTG<br /> | ||
+ | TTGTCCGTGTACCTGGTGCGGTTGAGTTACCGCTGGCTGCACGTCGTGTTGCCGCAAGTGGTAAGTTTGACGGTATTATTGCACTTGGTGCGGTAATCCG<br /> | ||
+ | TGGTGGTACCCCTCACTTTGATTTTGTTGCAGGCGAATGTAACAAAGGTCTGGCTCAAATTGCATTAGAGTTCGATCTGCCCGTTGCTTTCGGTGTATTG<br /> | ||
+ | ACTACAGATACCATTGAACAAGCTATTGAGCGTTCAGGTACTAAAGCGGGTAACAAAGGCGGCGAAGCTGCTCTAAGCTTGCTTGAAATGGTCAACGTTC<br /> | ||
+ | TGCAACAGCTTGAACAACAGTTGTAA<br /> | ||
+ | </p> | ||
+ | <p>>yieF chromate reductas 560 bp<br /> | ||
+ | ATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTG<br /> | ||
+ | CACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCC<br /> | ||
+ | CTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATC<br /> | ||
+ | CGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATG<br /> | ||
+ | CCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCGGTATTGATTCAGACCAGCTC<br /> | ||
+ | AATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATG<br /> | ||
+ | GTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGA<br /> | ||
+ | TTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAA<br /> | ||
+ | GATCTAA</p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
<p> </p> | <p> </p> | ||
+ | <p><script type="text/javascript"> | ||
+ | var _bdhmProtocol = (("https:" == document.location.protocol) ? " https://" : " http://"); | ||
+ | document.write(unescape("%3Cscript src='" + _bdhmProtocol + "hm.baidu.com/h.js%3F986011a32f84f432107ce07502765e79' type='text/javascript'%3E%3C/script%3E")); | ||
+ | </script></p> | ||
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<span class="li"><a href="http://en.lzu.edu.cn/">Lanzhou University</a></span> | <span class="li"><a href="http://en.lzu.edu.cn/">Lanzhou University</a></span> | ||
<span class="li"><a href="https://2013.igem.org/Team:LZU-China">LZU-CHINA 2013</a></span> | <span class="li"><a href="https://2013.igem.org/Team:LZU-China">LZU-CHINA 2013</a></span> |
Latest revision as of 03:58, 18 October 2014
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GEL PICTURES OF SOME PARTS |
Fig.2 The picture of K1523101. 1,3: pSB1C3 with K1523101 cut by EcoRI; 2,4: pSB1C3 with K11523101 cut by EcoRI and HindIII; 5: pSB1C3 with K1523101 not be cut.
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PARTS SEQUENCES |
>nahR naphthalene degradation LysR-family transcriptional activator 903bp
ATGGAACTGCGTGATCTGGATTTAAACCTGCTGGTGGTGTTCAACGAGCTGCTGGTCTGCAGACGCGTCTCTATCGTTGCCGAGAACCTGGGCCTGACCCAGCCTGCTGTCAGCAATGCGCTGAAACGCCTGCGCACAGCGCTACAGGACCAACTTTTCGTGCGCACCTACCAGGGTATGGAACCCACACCCTACGCCGCGAACTTGGCCGAGCCCGTCGCCTTGGCCATGCACGCCCTGCGCGAAGCCCTGCAGCACCATGAGCGCTTCGATCCGCTGACCAGCGAGCGTACTTTCACCCTGGCCATGACCGACATCGGCGAGATCTACTTCATGCCGCGGCTGATGGATGCGATTACTCTTCAAGCCCCCAATTGCGCGATCAGCACCGTGCGCGACAGTTCGATGAGCCTGATGCAAGCCTTGCAGAACGGCACAGTGGACCTAGCCGTGGGCCTGCTGCCCAACCTGCAGACTGGCTTCTTCCAGCGCCGGCTGCTCCACAATCACTACGTGTGCCTGTGTCGCAAGGACCATCCGGCCACCCGCGAACCCCTGACTCTTGAGCGCTTCTGTTCCTATGGCCACGTGCGTGTCATCGCCGCTGGCACAGGCCACGGCGAGGTGGACACATACTTGACGAAGGCCGGCATCCGGCGCGACATCCGCCTGGAGGTGCCGCACTTCGTCGCCGTTGGCCACATACTCCAGCGCACCGAGCTGATCGCCACTGTGCCGATACGTTTCGCCGACTGCTGCGTAGAGCCCTTCGGTCTGAGCGTCTTGCCGCACCCAGTCGCCTTGCCGGAAATCGCCATCAACATGTTCTGGCATGCGAAATACCACCAGGACCTCGCCAATATCTGGTTGCGGCAACTGATGTTCGACCTGTTTTCTGATTGA
>nahE trans-o-hydroxybenzylidenepyruvate hydratase-aldolase 1005bp
ATGTCGAATAAAATTATGAAAACGTCGCGTCTTACCGCCGAAGATATCAACGGCGCCTGGACTATAATGCCCACACCCTCGACGCCTGATGCTTCTGATTGGCGCAGCACTGCCACCGTGGACTTAGAAGAGACTGCCCGCATAGTTGAAGAGCTGATTGCAGCTGGTGTCAACGGTATTCTAAGTATGGGTACTTTTGGTGAGTGCGCCACGTTGACCTGGGATGAAAAACGTGATTATGTCTCGACGATTGTCGAGACCATTCGTGGTCGCGTGCCTTATTTCTGTGGCACGACAGCCTTAAATACCCGAGAAGTCATCCGCCAGACCCGAGAGCTTATCGATATTGGCGCCAACGGCACTATGCTCGGGGTGCCGATGTGGGTGAAGATGGACCTGCCTACAGCGGTTCAGTTCTATCGTGATGTTGCAGATGCGGTACCAGAGGCTGCCATTGCGATTTACGCCAACCCCGAAGCATTCAAGTTCGACTTCCCTCGCCCATTCTGGGCAGAGATGTCCAAAATTCCGCAGGTAGTGACTGCGAAGTATCTAGGCATCGGAATGCTTGACTTGGACCTGAGACTGGCACCCAACATCCGCTTCCTTCCCCACGAAGATGACTATTACGCGGCCGCACGCATCAATCCCGAGCGCATAACCGCGTTCTGGTCAAGCGGGGCCATGTGCGGCCCGGCTACCGCCATCATGTTGCGTGACGAAGTGGTGCGGGCCAAGAGCACCGGTGACTGGGCCAAGGCCAAAGCCATCTCCGATGATATGCGTGCAGCCGACTCGACATTGTTTCCGCGTGGCGACTTTTCGGAGTTCTCGAAGTATAATATCGGGCTTGAAAAGGCACGGATGGATGCGGCTGGTTGGCTCAAGGCTGGGCCCTGCCGTCCGCCCTACAACCTTGTTCCAGAAGACTACCTCGCTGGTGCACAGAAATCAGGCAAGGCTTGGGCCGCGCTGCACGCTAAATACAGTAATGAATTGAAGTAG
>nahG salicylate hydroxylase 1305bp
ATGAAAAACAATAAACCTGGCTTGCGCATCGGTATCATCGGCGGCGGGATTTCCGGCGTTGCCTTGGCTCTGGAGCTCTGTCGCTACTCCCATCTCCAAGTACAGCTGTTCGAGTGCGCGCCGGCTTTTGGTGAGGTCGGTGCAGGAGTTTCCTTTGGCCCCAACGCGGTGCGCGCCATTGTCGGCCTAGGCTTGGGCGAGGCCTACTTGCAGGTCGCCGACCGTACCTCGGAGCCCTGGGAGGACGTGTGGTTCGAGTGGCGGCGCGGCAGGGATGCCAGCTATCTGGGAGCGACCATCGCTCCGGGCGTGGGCCAGTCCTCGGTACACCGGGCGGATTTCCTCGATGCCCTAGTAAATCACCTCCCAAAAGGTATCGCCCAATTCAGGAAGCGCGCCACACAGGTCGAGCAAAAGGGGGGCGAAGTGCAAGTGCTATTCGCCGACGGCACAGAGCACCGCTGTGACCTTCTAATCGGTGCCGACGGAATCAAGTCAGCGCTGCGTAGTCATGTGCTGGAAGGTCAGGGGCTGGCCCCACAGGTACCGCGCTTCAGCGGCACCTGTGCCTATCGGGGGATGGTCGACAGCCTACACCTGCGCGAAGCCTATCGAGCCCAGGGCATCGACGAGCACTTGGTGGACGTACCGCAGATGTACCTAGGGCTTGACGGCCATATCCTCACTTTTCCGGTGAGGAATGGCCGCCTCATCAACGTTGTGGCCTTCATTTCCGACCGTAGTGAGCCGAAGCCGAACTGGCCCGCGGATGCCCCTTGGGTGCGCGATGTGAGCCAGCGAGAGATGCTCGATGCCTTCGCGGGTTGGGGTGATGCCGCGCGCACCCTGCTGGAGTGCATCCCGGCACCAACTCTCTGGGCACTGCACGACCTGGCGGAGCTGCCGGGCTACGTGCACGGGCGGGTCGTCCTGATCGGCGACGCAGCTCACGCCATGCTGCCGCACCAAGGCGCCGGTGCCGCCCAAGGACTTGAGGACGCCTACTTCCTCGCCCGCCTGTTGGGCGATACCCAAGTCGATGCAGACAACCTCGCCGAGCTGCTTGAAGCCTACGACGACCTGCGCCGCCCTCGTGCCTGTCGCGTGCAGCGAACCTCCCGGGAGACCGGCGAGTTATACGAGTTTCGCGACCCCGTCGTAGGTGCGAACGAGCATCTGCTGGGGGAAAACCTGGCGACCCGCTTCGACTGGCTGTGGAGCCACGACCTCGACGCCGACCTGGCCGAGGCACGTGCGCGCCTGGGTTGGGAAAATGGTAGCCGGGGTGTGCTACGTCAAGGGTGA
>ChrR chromate reductas 666 bp
ATGAAAAAAATAGTCCAGTCGGAAGGTTTTCGGTTCTCCGCTGGTCCAATTGTTCGCCCGATCATCTATAAATGGGGCTCCCTTGAACCCATCGTCGAGAAGTGTCCAATGAGTAATGTCTACACCGTGGCCGTTTTAGTGGGGAGCCTGCGCAAGGCTTCGATCAATCGCAAGCTGGCTCTGGCCCTGGCGGATCTGGCGCCGAGCAACCTGAAGCTGGAAATCATCGAGATAGGCGACCTGCCGCTCTACAACGAAGATATTGACGTCACACCCCCACCAGCCTACAGCACTTTCCGTGAGCAGGTTGGCGCGGCCGACGCATTGCTGTTTGTCACGCCTGAATACAACCGCTCGGTTCCGGCCCCGATGAAAAATGCCATTGACGTCGGCTCGCGGCCTTACGGCAAGAGCTGCCTGAGCGGTAAACCGGGTGCGGTGATCAGTGCCTCGCCGGGAGCCATGGGTGGTTTCGGCGCCAATCATCATTTGCGCCAGTCCCTGGTTTTCCTCGATGTGCCTTGCATGCAACAGCCGGAGGCTTATCTGGCCGGTGCGGGGAGCGCGTTCGATGAGCAGGGCGAGCTGTCTGAGTCGGTGAGGCCGTTTCTGCAGAAATTCATTGATGCCTATGGCAAGTGGGTTGAACAGCACAAAAAGGCCTGA
>BBa_K1523101 Part-only sequence (3726 bp)
TTCCCATCTATAATCCTCCCTGATTCTTCGCTGATATGGTGCTAAAAAGTAACCAATAAATGGTATTTAAAATGCAAATTATCAGGCGTACCCTGAAACG
TACTAGAGATTAAAGAGGAGAAATACTAGATGAATTGGTCAGTACTCGATAACCAAATGATGAGCCGAGCTATTCAATTGGCTCGCAAAGGATTTTATAC
CACTCGCCCCAATCCCAGTGTGGGCTGCGTTATCGTTAACGATAACCAGATTGTCGGTGAAGGTTATCATCAAAAAGCCGGAGAGCCCCACGCTGAAGTG
CATGCGCTGCGCATGGCTGGCGAGCTTGCCCGTGGCGCGACTGCTTATGTCACCTTAGAACCCTGTAGCCATTATGGTCGTACGCCGCCCTGCGCCTTGG
CGCTGATCAATATTGGTGTAAAGCGGGTGGTGGTGGCGGTTGAAGATCCTAATCCGCAGGTCGGCGGGCGCGGTATTCAAATGCTGCGCGATGCGGGTAT
TCAAGTGGATGTGGGCTTGCATCGTGATGAGGCCTATGCCTTAAATCTTGGTTTTATGAAGCGCATGGAGTCGGGTCTACCTTGGGTGACGGTAAAGCTT
GCCGCGAGTCTTGATGGTAAAACTGCGTTATCAAACGGGGTGTCTAAATGGATCACTGGCCCTGAAGCTCGCCGCGATGTGCAGCGCTTAAGATTACGAT
CCTGCGCGCTGATCACGGGGATTGAAACGGTACTGGCCGATGATCCATCGCTCAATGTGCGCTACTCAGAGCTTGGCAGTTTAAGAGCGCAATTGAGTGA
GTCGCAGGTTTTACAGCCGCTGCGAGTGGTTTTGGACAGTCGCGCCCGCATGCCTATCACGGCAGCATTGTTTGCGATTGAATCACCCATTTTATTAGTC
TCGACAGTGAGCTATTCGTCAGACTTTATGGTGCAATTACCCGCCCATGTGACTTGCCTCCAATTACCAGCCATTGAGGGTCGAATCTCCTTGTCTGCAC
TATTAAGCTATTTAGGCAAAAGCTGTAATCAGGTGCTTATCGAAGCGGGCGCGACTTTAGCTGGCGCCTTTATCGGCTCAGGATTAGCCGATGAATTAGT
GCTTTATCAAGCGATGAAAATCCTTGGCGGCCAAGGACGTAATCTACTTGAATTACCCGATTATCAAATGATGGCCGATATTCCGACCCTCAAATTGGTC
GACGAGCGTAAAGTGGGCGCGGATACGCGCTTCACCTTGAGGCTCGAGTCCAATCCATCTTTAGCGAATAAGTGAGTTAACCATGTTTACTGGGATTATT
GAGGCCGTTGGTACACTGCGAAAGCTTGAGCGTAAAGGCGATGATATTCGCTTAACGGTCGCCAGTGGCAAACTGGATTTAACCGATGTGCGTTTAGGCG
ACAGTATCGCCACCAACGGTGTATGTTTGACCGTGGTTCAACAGTTAGCCGATGGCTATGTGGCTGATGTGTCAGCTGAAACCGTCAGCCTTACAGGTTT
TGCAAACTATAAAGTGGGTACTAAGGTCAATCTTGAAAAGGCCGTCACCCCAACTACTCGTTTAGGCGGCCATATGGTGAGTGGTCATGTGGATGGGATT
GCTACTGTTGAGCAGCGTCTTGCTCGTGGTCAAGCAATCGAATTTTGGTTAGCGGCTCCCACGGAGCTTGCTCGCTATATTGCCCATAAAGGTTCTATCA
CGATTGATGGCGTGAGCCTGACAGTAAACGAAGTCGATGGACACCGTTTTCGATTAACGATTGTGCCCCATACTGTAGGTGAAACCACGTTGGTGGATTT
AAAAGCAGGCGATAAGGTCAATATTGAAGTGGACTTAATTGCCCGTTATTTAGAGCGTTTAATGCGTTTTGACAGCAAAGAAACCCAAGGCGGAGGGGTG
ACCATGGAAATGTTAGCCCGTGCTGGCTTTGTGCGTTAGTACACTGGCACTCAGTTCACTTAGGTATAGAATTTATAACAACAGTCACATTATAAAGGTC
CTACAATGGCGCTGCACAGTATAGAAGAGATCATCGAGGATATTCGTCAAGGCAAAATGGTTATTTTGATGGATGACGAGGACAGAGAAAACGAAGGCGA
TCTGATTTTGGCGGCCGAAATGGTGACGCCAGAAGCGATTAACTTTATGGCGAAATACGGCCGCGGGTTAATCTGCCAAACGATGACCAAGGCCCGTTGC
CAGCAGTTAAATCTGCCCTTGATGGTAACCAATAACAATGCGCAGTTCTCGACCAACTTTACGGTATCGATTGAAGCTGCCGAAGGCGTAACCACAGGGA
TTTCGGCCCACGATCGCGCTGTAACCGTTAAAGCGGCCGTAGCAAAAGATGCTAAAGCGTCGGACTTAGTTCAGCCTGGGCATATCTTCCCGTTAATGGC
GCAGGATGGTGGCGTATTAACCCGCGCAGGTCACACTGAAGCGGGCTGTGATTTAGCCCGTTTAGCAGGGCTTGAACCATCGGGGGTGATTGTTGAAATT
TTGAACGAAGACGGCACTATGGCCCGTCGCCCAGATTTAGAGATTTTCTCTGAAAAACATGGCATTAAAATCGGCACTATTGCGGCCCTGATTGAATACC
GTAATACCAAAGAAACGACCGTTGTGCGTGAAGCCAAATGCAAACTGCCAACCCGTTTCGGTGAGTTCGATATGGTGACCTTTAGGGATACTATCGACAA
TCAATTGCACTTTGCCTTAGTGAAAGGCGAGGTGAAGCCTGACTGTTTAGTACGGGTACATTTACAGAATACCTTTAACGATCTACTTCACTCTGAGCGT
GATCAGCAACGTAGCTGGCCGCTCGAAAAAGCGATGGCGCGGATTTCTGCCGAAGGTGGTGTATTAGTACTGCTTGGCAATCAGGAACATTCCAGTGAAA
TCCTCGCCAAAGTCAAAGCCTTTGAAGCGGAAGATCAAGGTCAGACCCCAGTATCGGCTAAATGGCAAGGCACCTCACGCCGTGTAGGTGTGGGTTCGCA
AATCCTCGCCAGTTTAGGCGTGACAAAAATGCGACTCTTAAGCTCGCCCAAACGTTATCACTCACTTTCAGGCTTTGGCCTTGAAGTGACCGAATGCGTA
GCTGAGTAACAGGTTAAGCTTGTAATAGGTTGCACTAAGGTAAAATTAACCATTTCTATCATTTGCATAAGGCCGCGGGCAATTGACTGTGGTATCATGT
CGCCACTTTTCGCCCAGAGCTGGGTGCTTTAGCTAAATTAGGTAAGAAAATGAACGTAGTTCAAGGTAATATCGAAGCGAAGAATGCCAAAGTTGCGATT
GTAATTTCGCGTTTTAACAGCTTTTTAGTCGAAAGCCTGCTTGAAGGTGCACTTGACACGCTGAAACGTTTTGGCCAAGTCAGTGATGAAAACATCACTG
TTGTCCGTGTACCTGGTGCGGTTGAGTTACCGCTGGCTGCACGTCGTGTTGCCGCAAGTGGTAAGTTTGACGGTATTATTGCACTTGGTGCGGTAATCCG
TGGTGGTACCCCTCACTTTGATTTTGTTGCAGGCGAATGTAACAAAGGTCTGGCTCAAATTGCATTAGAGTTCGATCTGCCCGTTGCTTTCGGTGTATTG
ACTACAGATACCATTGAACAAGCTATTGAGCGTTCAGGTACTAAAGCGGGTAACAAAGGCGGCGAAGCTGCTCTAAGCTTGCTTGAAATGGTCAACGTTC
TGCAACAGCTTGAACAACAGTTGTAA
>yieF chromate reductas 560 bp
ATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTG
CACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCC
CTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATC
CGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATG
CCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCGGTATTGATTCAGACCAGCTC
AATGGGCGTGATTGGCGGCGCGCGCTGTCAGTATCACCTGCGCCAGATTCTGGTTTTCCTCGATGCAATG
GTGATGAACAAGCCGGAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGA
TTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAA
GATCTAA