Team:LZU-China/Safety

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             <td width="18"><p align="center"><strong>&nbsp;</strong></p></td>
             <td width="18"><p align="center"><strong>&nbsp;</strong></p></td>
             <td width="89"><p align="center"><strong>Species</strong></p></td>
             <td width="89"><p align="center"><strong>Species</strong></p></td>
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             <td width="67"><p align="center"><strong>Strain   no/name</strong></p></td>
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             <td width="67"><p align="center"><strong>Strain </strong>No/name</strong></p></td>
             <td width="48"><p align="center"><strong>Risk Group</strong></p></td>
             <td width="48"><p align="center"><strong>Risk Group</strong></p></td>
             <td width="516"><p align="center"><strong>Disease    risk to humans? If so, which disease</strong></p></td>
             <td width="516"><p align="center"><strong>Disease    risk to humans? If so, which disease</strong></p></td>
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             <td><p align="left">MR1</p></td>
             <td><p align="left">MR1</p></td>
             <td><p align="left"><a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=&amp;species=coli">1</a></p></td>
             <td><p align="left"><a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=&amp;species=coli">1</a></p></td>
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             <td><p align="left">No</p></td>
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             <td><p align="left">No, S. oneidensis MR-1 belongs to a class of bacteria known as "Dissimilatory Metal-Reducing Bacteria (DMRB)" because of their ability to couple metal reduction with their metabolism. It cannot cause diseases</p></td>
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             <td width="15"><p align="left">&nbsp;2</p></td>
             <td width="15"><p align="left">&nbsp;2</p></td>
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             <td width="96"><p align="left">BBA_K1523101</p></td>
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             <td width="96"><p align="left"><a href="http://parts.igem.org/Part:BBa_K1523101">K1523101</p></td>
             <td width="131"><p align="left">From    distributed kit of iGEM 2013<br />
             <td width="131"><p align="left">From    distributed kit of iGEM 2013<br />
               BBA&shy;_K381001</p></td>
               BBA&shy;_K381001</p></td>
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Latest revision as of 14:31, 3 December 2014

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" " http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> LZU-China 2014

 
 
          SAFETY

 

 

          

          

 

Species

Strain No/name

Risk Group

Disease risk to humans? If so, which disease

 1

E. coliK 12

DH5 alpha

1

Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E. coli K-12 is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections.

 2

Shewenella Onedensis

MR1

1

No, S. oneidensis MR-1 belongs to a class of bacteria known as "Dissimilatory Metal-Reducing Bacteria (DMRB)" because of their ability to couple metal reduction with their metabolism. It cannot cause diseases

 

 

 

Part number

Where did us get the physical DNA for this part

What species does this part originally come from?

 

Natural function of part

What is the Risk Group of the species?

How will you use it?

 1

pSB1C3

From Li Xiangkai's Lab, Lanzhou University

Escherichia coli K-12

Vector for Escherichia coli

     1
  1. Vector for E.coli to express BBA_K1523101
  2. Submission vector for all parts

 2

K1523101

From distributed kit of iGEM 2013
BBA­_K381001

Escherichia coli K-12

Detector of Nitrate

     1

1.  Strain used in anode of MFC 2.  Detector of PNP
3.  Riboflavin producing

 3

pET-28(a) +

From Li Xiangkai's Lab, Lanzhou University

Vector for Escherichia coli

Vector for Escherichia coli

     1

Vector for E.coli to express BBA_K1523101

 4

BBa_K1523008

Lanzhou University

Escherichia coli

Chromate reduction

     1

1.  Strain used in anode of MFC 2.  Chromate reduction in anode

 5

BBa_K1523021

Lanzhou University

Pseudomonas fluorescens

Chromate reduction

     1

1.  Strain used in cathode of MFC
2.  Other organic pollutant reduction in cathode

 6

BBa_K1523022

Lanzhou University

Pseudomonas fluorescens

Chromate reduction

     1

1.  Strain used in cathode of MFC
2.  Other organic pollutant reduction in cathode

 7

BBa_K1523023

Lanzhou University

Pseudomonas fluorescens

Chromate reduction

     1

1.  Strain used in cathode of MFC
2.  Other organic pollutant reduction in cathode

 8

BBa_K1523025

Lanzhou University

Pseudomonas putida

Chromate reduction

     1

1.  Strain used in anode of MFC 2.  Chromate reduction in anode

 

        We have taken safety courses before so the entire group members know how to protect ourselves in experiments.
        As for environment, some of the masses hesitate it may cause genetic pollution to the environment, but it should be noticed that the gene modified E.coli we construct cannot express our target gene unless it is stimulated by the pollutant PNP.

 

 

 

 

 

 

 

 


  

 

 

 
 
 

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