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Team:TU Darmstadt/Notebook/Labjournal/K1497006
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| - | <ul class="menu"><li class="first"><a href=" | + | <ul class="menu"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt" >Home</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Project" >Project</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results" >Results</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/PolicyandPractices" >Policy & Practices</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Achievements" >Achievements</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook" >Notebook</a><ul class="menu2"><li class="active first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal" >Labjournal</a><ul class="menu3"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497000" >K1497000</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497001" >K1497001</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497002" >K1497002</a></li><li ><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497003" >K1497003</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497004" >K1497004</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497005" >K1497005</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497006" >K1497006</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497007" >K1497007</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497008" >K1497008</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497009" >K1497009</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497010" >K1497010</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497011" >K1497011</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497012" >K1497012</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497013" >K1497013</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497014" >K1497014</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497015" >K1497015</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497016" >K1497016</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497017" >K1497017</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497018" >K1497018</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497019" >K1497019</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497020" >K1497020</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497021" >K1497021</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497022" >K1497022</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497023" >K1497023</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497024" >K1497024/5/6</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497027" >K1497027/28/29/30</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497031" >K1497031</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497032" >K1497032</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497033" >K1497033</a></li></ul></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials" >Materials</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods" >Methods</a></li></ul></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Team" >Team</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Gallery" >Gallery</a></li></ul> |
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| - | <!--TYPO3SEARCH_begin--><div id="c115" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">K1497006 - Composite part of 4Cl and TAL with strong RBS (B0034-4Cl-B0034-TAL)</h1></div><div class="csc-textpic-text"><div><p>For combing two of our Biobricks, we digested B0034-4CL-pSB1C3 with EcoRI + SpeI and B0034-TAL-pSB1C3 with EcoRI + XbaI. Furthermore we dephosporylated the digestion of B0034-TAL-pSB1C3. We ligated both digestions with T4 ligase and transformed the product into Top10 E.coli via heat shock. We spread out on CMP-LB agar plates and incubated the plates overnight at 37°C. We analyzed obtained colonies by colony PCR and agarose gel electrophoresis. Positive colonies were inoculated in 5 ml LB with CMP and plasmids were isolated after incubating for 16 h at 37°C.</p></div><div></div></div></div><!--TYPO3SEARCH_end--> | + | <!--TYPO3SEARCH_begin--><div id="c115" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">K1497006 - Composite part of 4Cl and TAL with strong RBS (B0034-4Cl-B0034-TAL)</h1></div><div class="csc-textpic-text"><div><p>For combing two of our Biobricks, we digested B0034-4CL-pSB1C3 with EcoRI + SpeI and B0034-TAL-pSB1C3 with EcoRI + XbaI. Furthermore we dephosporylated the digestion of B0034-TAL-pSB1C3. We ligated both digestions with T4 ligase and transformed the product into Top10 E.coli via heat shock. We spread out on CMP-LB agar plates and incubated the plates overnight at 37°C. We analyzed obtained colonies by colony PCR and agarose gel electrophoresis. Positive colonies were inoculated in 5 ml LB with CMP and plasmids were isolated after incubating for 16 h at 37°C gaining the BioBrick <a href="http://parts.igem.org/Part:BBa_K1497006" title="Opens internal link in current window" class="internal-link"> BBa_K1497006</a>.</p></div><div></div></div></div><!--TYPO3SEARCH_end--> |
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Latest revision as of 18:49, 17 October 2014
K1497006 - Composite part of 4Cl and TAL with strong RBS (B0034-4Cl-B0034-TAL)
For combing two of our Biobricks, we digested B0034-4CL-pSB1C3 with EcoRI + SpeI and B0034-TAL-pSB1C3 with EcoRI + XbaI. Furthermore we dephosporylated the digestion of B0034-TAL-pSB1C3. We ligated both digestions with T4 ligase and transformed the product into Top10 E.coli via heat shock. We spread out on CMP-LB agar plates and incubated the plates overnight at 37°C. We analyzed obtained colonies by colony PCR and agarose gel electrophoresis. Positive colonies were inoculated in 5 ml LB with CMP and plasmids were isolated after incubating for 16 h at 37°C gaining the BioBrick BBa_K1497006.
