Team:Tongji/human-practice

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                 <a  href="https://2014.igem.org/Team:Tongji/project">Project</a>
                 <a  href="https://2014.igem.org/Team:Tongji/project">Project</a>
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                 <a  href="https://2014.igem.org/Team:Tongji/human-practice">Human Practice</a>
                 <a  href="https://2014.igem.org/Team:Tongji/human-practice">Human Practice</a>
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                 <a  href="https://2014.igem.org/Team:Tongji/safety">Safety</a>
                 <a  href="https://2014.igem.org/Team:Tongji/safety">Safety</a>
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                 <a  href="https://2014.igem.org/Team:Tongji/honors-room">Honors Room</a>
                 <a  href="https://2014.igem.org/Team:Tongji/honors-room">Honors Room</a>
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                 <a href="javascript:void(0)" class="dropdown-toggle" data-toggle="dropdown" >Language <b class="caret"></b></a>
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                   <li ><a  href="https://2014.igem.org/Team:Tongji/honors-room"  >English</a></li>
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                   <li ><a  href="https://2014.igem.org/Team:Tongji/human-practice"  >English</a></li>
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                   <li role="presentation"><a href="https://2014.igem.org/Team:Tongji/honors-room-zh">中文</a></li>
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                   <li role="presentation"><a href="https://2014.igem.org/Team:Tongji/human-practice-zh">中文</a></li>
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                 <a href="javascript:void(0)" class="dropdown-toggle" data-toggle="dropdown" >iGEM Bar <b class="caret"></b></a>
                 <a href="javascript:void(0)" class="dropdown-toggle" data-toggle="dropdown" >iGEM Bar <b class="caret"></b></a>
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                   <li ><a  href="/wiki/index.php?title=Talk:Team:Tongji/honors-room&amp;action=edit&amp;redlink=1">Discussion</a></li>
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                   <li ><a  href="/wiki/index.php?title=Talk:Team:Tongji/human-practice&amp;action=edit&amp;redlink=1">Discussion</a></li>
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                   <li ><a  href="/wiki/index.php?title=Team:Tongji/human-practice&amp;action=edit">Edit</a></li>
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                   <li ><a  href="/wiki/index.php?title=Team:Tongji/honors-room&amp;action=history">History</a></li>
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                   <li ><a  href="/wiki/index.php?title=Team:Tongji/human-practice&amp;action=history">History</a></li>
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                   <li ><a  href="/wiki/index.php?title=Team:Tongji/human-practice&amp;action=watch">Watch</a></li>
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                   <li ><a href="/wiki/index.php?title=Special:UserLogin&amp;returnto=Team:Tongji">Login</a></li>
                   <li ><a href="/wiki/index.php?title=Special:UserLogin&amp;returnto=Team:Tongji">Login</a></li>
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          <li><a href="#html-template2"><i class="icon-chevron-right"></i> Help another team</a></li>
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             <h1>Mini Showroom</h1>
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          <p class="lead">Available during iGEM Jamboree. Please stay tuned.</p>
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                                <td><div >Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry </div></td>
 
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                                <td><div >①ManA1 ②ArfB ③XynB </div></td>
 
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                                <td><div >Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected. </div></td>
 
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                                <td><div ></div>Validation experiment conducted, please see Lab Archives.</td>                           
 
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                                <td><div >Document the characterization of this part in the “Main Page” section of that Part’s/Device’s Registry entry. </div></td>
 
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                                <td><div ></div>Please see Lab Archives.</td>                           
 
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                                <td><div >Submit this new part to the iGEM Parts Registry</div></td>
 
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                                <td><div ></div>Please see Lab Archives.</td>                           
 
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                                <td><div >Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project.</div></td>
 
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                                <td><div ></div>Please see Safety.</td>                           
 
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                                <td><div >Improve the function of an existing BioBrick Part or Device </div></td>
 
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                                <td><div >Synergy exploration</div></td>
 
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                                <td><div >Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system. </div></td>
 
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                                <td><div >Fudan owes us a big favour for lentivirus vector we gave them.</div></td>
 
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            <h1>Attributions</h1>
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          <p class="lead">Please enjoy our story.</p>
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          <p class="lead">Basically, we are a self-funding team. Three of our team members paid the registration fee of $3500 at first, and then our college became willing to give us a experimental funding of $5000. And we paid the rest.</p>
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          <p class="lead">We are an independent group. We raised our own idea, we modified our idea, and we troubleshot the problems we encountered. And the reason we can technically survive without help from outside is we have talented core members, especially Mr.Jiang, who read mountains of papers and found solutions to many obstacles.</p>
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          <p class="lead">Almost all we present here, come from our team members officially listed. Technically, we don't have to say thank-you to someone from outside. You can find the detailed work of our team members below.</p>
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             <h1>Outreach</h1>
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          <p class="lead">We tightly communicate with several iGEM teams to improve our idea and appreciate their work.</p>
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           <h3>With Shanghai JiaoTong University</h3>
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                  <p>On 19th April, we invited SJTU to have a mini iGEM communication meeting with us.</p>
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                   <p>Provide them with the plasmid vectors</p>
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                   <p>Our guests helped analyse which project is significant to the reality and can be achieved.</p>
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                  <p>Members from Tongji University shared their opinions upon the last year's iGEM projects.</p>
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              <div class="item"><img src="https://static.igem.org/mediawiki/2014/b/b5/SJTU4.JPG" alt="">
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                  <p>What’s your expectation for iGEM? What should an iGEM team do? What’s the challenge? </p>
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                  <p>What’s synthetic biology? How is it developing currently? What's the trend?</p>
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              <div class="item"><img src="https://static.igem.org/mediawiki/2014/9/9c/SJTU6.JPG" alt="">
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                  <p>Members from Tongji University shared their opinions upon the last year's iGEM projects.</p>
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                  <p>How to lead a team? How to work efficiently?</p>
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              <div class="item"><img src="https://static.igem.org/mediawiki/2014/0/03/SJTU8.JPG" alt="">
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                  <p>Members from Tongji University shared their opinions upon the last year's iGEM projects.</p>
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 +
          <p class="lead">On 19th April, we invited members from Shanghai Jiao Tong University(SJTU) to have a mini iGEM communication meeting with us.</p>
 +
          <p class="lead">During the meeting, SJTU introduced iGEM competition and synthetic biology, shared their experience in team management, and discussed some last year's iGEM projects .</p>
 +
          <p class="lead">Thank SJTU for giving us a mini but meaningful iGEM communication meeting. We are looking forward to our further collaboration.</p>
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           <h3>With Fudan University</h3>
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                   <p>On 8th June, We had another communication meeting with team Fudan.</p>
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                        Jian Gao 高健
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                        Carried out the pilot experiment of in-lab paper making process.
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                        Liang Jiang 姜亮
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                        Carried out more than 80% of total experiment with Yixiao Zhou, including PCR, vector construction, transfection, positive selection, expression and purification of enzyme, bio-chemical validation of enzyme. He deserved to be our prior scientis, since he spent much of his private time and even working time on our project, and made several important decisions.
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                        Ling Leng 冷泠
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                        Participated in paper decoloring experiment and expression of enzyme.
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                        NanFang Hong 洪楠方
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                        Project initiator and investor, responsible for internal communication, team design and development of wiki. Participated in paper decoloring experiment.
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                        Shisheng Zhang 张仕晟
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                        Project initiator and investor, undertook the the complex administrative and logistics work.
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                        Yixiao Zhou 周一枭
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                        Project initiator and investor, Carried out more than 80% of total experiment with Liang Jiang, including PCR, vector construction, transfection, positive selection, expression and purification of enzyme, bio-chemical validation of enzyme.
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                        Yu Chen 陈御
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                        Undertook part of administrative work and participated paper decoloring experiment. And provided important suggestion on many occasions.  
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                        Yue Qi 祁玥
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                        Carried out the cultivation of our thermophilic gene host bacterium (<em>Clostridium stercorarium</em>). Responsible for paper decoloring experiment and Safety paperwork.
+
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                        Zixuan Zhang 张子轩
+
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                        Participated expression of enzyme.
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                  <p>Team Fudan's project idea was fantastic!</p>
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                        Mengjie Zhang 张梦杰
+
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                        Our advisor through the whole project. She intended to give us the chances to explore by our own, instead of feeding us the answer. We harvested a lot! Thank you!
+
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                   <p>They said they need certain vector in their project.</p>
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                        Chenxi Jin 金晨希
+
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                        Designed Synergy and Hot Resistance icons.
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                        Haiyan Li 李海燕
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                        Designed Eco Friendly icon.
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                        Siyuan Chen 陈思源
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                        Designed Decolor Four cartoons and their bottle labels.
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                  <p>We are happy to help them.</p>
-
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                        Xinhong Chen 陈欣泓
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                      </a>
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                        Inspired to join the next iGEM event.
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                  </div>
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                      <a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion4" href="#collapseTwo4">
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                        Yu Feng 冯羽
+
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                      </a>
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                        Inspired to join the next iGEM event.
+
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                      </div>
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                  </div>
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                <p>Etsy mixtape wayfarers, ethical wes anderson tofu before they sold out mcsweeney's organic lomo retro fanny pack lo-fi farm-to-table readymade. Messenger bag gentrify pitchfork tattooed craft beer, iphone skateboard locavore carles etsy salvia banksy hoodie helvetica. DIY synth PBR banksy irony. Leggings gentrify squid 8-bit cred pitchfork. Williamsburg banh mi whatever gluten-free, carles pitchfork biodiesel fixie etsy retro mlkshk vice blog. Scenester cred you probably haven't heard of them, vinyl craft beer blog stumptown. Pitchfork sustainable tofu synth chambray yr.</p>
 
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                <p>Trust fund seitan letterpress, keytar raw denim keffiyeh etsy art party before they sold out master cleanse gluten-free squid scenester freegan cosby sweater. Fanny pack portland seitan DIY, art party locavore wolf cliche high life echo park Austin. Cred vinyl keffiyeh DIY salvia PBR, banh mi before they sold out farm-to-table VHS viral locavore cosby sweater. Lomo wolf viral, mustache readymade thundercats keffiyeh craft beer marfa ethical. Wolf salvia freegan, sartorial keffiyeh echo park vegan.</p>
 
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 +
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 +
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 +
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 +
          <p class="lead">On 8th June, We had another communication meeting with team Fudan. During our meeting, we introduced each other's project, and raised suggestion to the projects.</p>
 +
          <p class="lead">At the end, team Fudan seeked <a href="./human-practice.html#html-template2">help</a> from us, and we did all we can to help them.</p>
 +
 
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+
             <h1>Video</h1>
           </div>
           </div>
 +
          <p class="lead">You can watch our video on Youtube or Youku, or download from <a  onclick=window.parent.location.href="https://vimeo.com/108265800"><strong>vimeo</strong></a>. Please enjoy our video! </p>
 +
          <div class="container span9">
 +
            <a  class="thumbnail span4" onclick=window.parent.location.href="http://v.youku.com/v_show/id_XNzk4ODE3NDA0.html">
 +
              <img  alt="260x120" src="https://static.igem.org/mediawiki/2014/1/17/Youku.png" style="width: 260px; height: 120px;">
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 +
            <a  class="thumbnail span4" onclick=window.parent.location.href="http://youtu.be/gCHqclyXfXI">
 +
              <img  alt="260x120" src="https://static.igem.org/mediawiki/2014/c/c2/Youtube-Tongji.png" style="width: 260px; height: 120px;">
 +
            </a>
 +
          </div>
 +
          <br><br><br><br><br><br><br>
            
            
-
            <p class="lead">Thank our college for giving us ¥30,000 Funding. (Special thank to professor <strong>Zhiwei Cao</strong>)</p>
+
 
-
            <p class="lead">Thank our dear professor <strong>FangLin Sun</strong>, <strong>Jianmin Fang</strong> and <strong>Jian Fei</strong>. They provided their lab place, equipments and reagent for us to carry out the experiment.</p>
+
-
            <p class="lead">Thank to our graphic designers: <a id="Jin" href="#" rel="tooltip" data-placement="top" data-original-title="Designed Synergy and Hot Resistance icons."><strong>Chenxi Jin</strong></a>, <a id="Li" href="#" rel="tooltip" data-placement="top" data-original-title="Designed Eco Friendly icon."><strong>Haiyan Li</strong></a>, <a id="Chen" href="#" rel="tooltip" data-placement="top" data-original-title="Designed Decolor Four cartoons and their bottle labels."><strong>Siyuan Chen</strong></a>.</p>
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         </section>
         </section>
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        <!-- HTML template 2
 +
        ================================================== -->
 +
        <section id="html-template2">
 +
          <div class="page-header">
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            <h1>Help another team</h1>
 +
          </div>
 +
          <p class="lead">During this summer, we provided assistance within our capacity to <a href="https://2014.igem.org/Team:Fudan">Fudan</a>, another iGEM team. Fudan also <a id="Jin" href="#" rel="tooltip" data-placement="top" data-original-title="①In our wiki, we use the Bootstrap framework which was recommended by Zhu Liu in Team Fudan 
 +
            ②Team Fudan gave us guildance about how to deal with the iGEM package. 
 +
            ③We were persuaded out of our first idea by team Fudan. 
 +
            ④They told us the name of professors who studied thermal stability in Fudan when we said we need to optimize the thermal stability of our enzyme"><strong>help</strong></a> us.</p>
 +
         
 +
 +
          <div id="myCarousel4" class="carousel slide">
 +
            <ol class="carousel-indicators">
 +
              <li data-target="#myCarousel4" data-slide-to="0" class="active"></li>
 +
              <li data-target="#myCarousel4" data-slide-to="1"></li>
 +
              <li data-target="#myCarousel4" data-slide-to="2"></li>
 +
            </ol>
 +
            <!-- Carousel items -->
 +
            <div class="carousel-inner">
 +
              <div class="active item"><img src="https://static.igem.org/mediawiki/2014/8/82/HelpFDU1.JPG" >
 +
                <div class="carousel-caption">
 +
                  <p>Our professor gave them instruction and resources</p>
 +
                </div>
 +
              </div>
 +
 +
              <div class="item"><img src="https://static.igem.org/mediawiki/2014/5/5b/HelpFDU2.jpg" >
 +
                <div class="carousel-caption">
 +
                  <p>Provide some significant information about cell cultivation</p>
 +
                </div>
 +
              </div>
 +
              <div class="item"><img src="https://static.igem.org/mediawiki/2014/4/4a/HelpFDU3.jpg" >
 +
                <div class="carousel-caption">
 +
                  <p>Provide them with the plasmid vectors</p>
 +
                </div>
 +
              </div>
 +
            </div>
 +
            <!-- Carousel nav -->
 +
            <a class="carousel-control left" href="#myCarousel4" data-slide="prev">&lsaquo;</a>
 +
            <a class="carousel-control right" href="#myCarousel4" data-slide="next">&rsaquo;</a>
 +
          </div>
 +
          <br>
 +
          <br>
 +
          <p class="lead">We offer help to Fudan University on their iGEM project, including:</p>
 +
 +
          <table class="table table-hover">
 +
            <thead>
 +
              <tr>
 +
                <th class="text span2">Assistance</th>
 +
                <th class="text span2">Item</th>
 +
                <th class="text">Detail</th>
 +
 +
              </tr>
 +
            </thead>
 +
            <tbody>
 +
              <tr>
 +
                <td><img src="https://static.igem.org/mediawiki/2014/2/2e/Star.png"><img src="https://static.igem.org/mediawiki/2014/2/2e/Star.png"><img src="https://static.igem.org/mediawiki/2014/2/2e/Star.png"></td>
 +
                <td><div >Provide a plasmid vector named ‘fvw-tet-oskm’</div></td>
 +
                <td><div >It is a plasmid containing the four Yamanaka factors(Oct4, Sox2, Klf4 and c-Myc), and the factors are connected with a T2A linker. It can be used to induce iPSCs.</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td><img src="https://static.igem.org/mediawiki/2014/2/2e/Star.png"><img src="https://static.igem.org/mediawiki/2014/2/2e/Star.png"><img src="https://static.igem.org/mediawiki/2014/2/2e/Star.png"></td>
 +
                <td><div >Provide a plasmid vector named ‘fvw-tet-sox2’</div></td>
 +
                <td><div >It is a plasmid containing the gene, Sox2. It can be used to induce neuron stem cells.</div></td>
 +
              </tr>
 +
              <tr>
 +
                <td><img src="https://static.igem.org/mediawiki/2014/2/2e/Star.png"><img src="https://static.igem.org/mediawiki/2014/2/2e/Star.png"></td>
 +
                <td><div >Provide some significant information about cell cultivation</div></td>
 +
                <td><div >We provide the recipe of medium and the protocol for cultivating for neuron cells, iPSCs
 +
                  and neuron stem cells.</div></td>
 +
                </tr>
 +
              <tr>
 +
                  <td><img src="https://static.igem.org/mediawiki/2014/2/2e/Star.png"></td>
 +
                  <td><div >Help to solve some detailed problems</div></td>
 +
                  <td><div >We also help them to solve some detailed problems, such as helping to choose markers for iPSc screening and recommending kits and reagents for their experiments.</div></td>
 +
              </tr>
 +
            </tbody>       
 +
 +
          </table>
 +
 +
 +
 +
        </section>
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Latest revision as of 05:47, 17 October 2014

iGEM Tongji 2014

Human Practice

Mini Showroom

Available during iGEM Jamboree. Please stay tuned.

Outreach

We tightly communicate with several iGEM teams to improve our idea and appreciate their work.

With Shanghai JiaoTong University



On 19th April, we invited members from Shanghai Jiao Tong University(SJTU) to have a mini iGEM communication meeting with us.

During the meeting, SJTU introduced iGEM competition and synthetic biology, shared their experience in team management, and discussed some last year's iGEM projects .

Thank SJTU for giving us a mini but meaningful iGEM communication meeting. We are looking forward to our further collaboration.

With Fudan University



On 8th June, We had another communication meeting with team Fudan. During our meeting, we introduced each other's project, and raised suggestion to the projects.

At the end, team Fudan seeked help from us, and we did all we can to help them.

Video

You can watch our video on Youtube or Youku, or download from vimeo. Please enjoy our video!

260x120 260x120







Help another team

During this summer, we provided assistance within our capacity to Fudan, another iGEM team. Fudan also help us.



We offer help to Fudan University on their iGEM project, including:

Assistance Item Detail
Provide a plasmid vector named ‘fvw-tet-oskm’
It is a plasmid containing the four Yamanaka factors(Oct4, Sox2, Klf4 and c-Myc), and the factors are connected with a T2A linker. It can be used to induce iPSCs.
Provide a plasmid vector named ‘fvw-tet-sox2’
It is a plasmid containing the gene, Sox2. It can be used to induce neuron stem cells.
Provide some significant information about cell cultivation
We provide the recipe of medium and the protocol for cultivating for neuron cells, iPSCs and neuron stem cells.
Help to solve some detailed problems
We also help them to solve some detailed problems, such as helping to choose markers for iPSc screening and recommending kits and reagents for their experiments.

Tongji University, 2014

Retrieved from "http://2014.igem.org/Team:Tongji/human-practice"