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From 2014.igem.org

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(93 intermediate revisions not shown)
Line 12:		Line 12:
	<!--サイドメニュー-->		<!--サイドメニュー-->
	<ul id="nav5">		<ul id="nav5">
-	<li class="menuimg"><a href="/Team:KIT-Kyoto/Test">home</a></li>	+	<li class="menuimg"><a href="/Team:KIT-Kyoto">home</a></li>
-	<li class="menuimg"><a href="/Team:KIT-Kyoto/Test/About">about us</a>	+	<li class="menuimg"><a href="/Team:KIT-Kyoto/About">about us</a>
-	<li class="menuimg"><a href="/Team:KIT-Kyoto/Test/Project">project</a></li>	+	<li class="menuimg"><a href="/Team:KIT-Kyoto/Project">project</a></li>
-	<li class="menuimg"><a href="/Team:KIT-Kyoto/Test/Modeling">Modelling</a></li>	+
	<li class="menuimg"><a href="/Team:KIT-Kyoto/HumanPractice">policy & practices</a></li>		<li class="menuimg"><a href="/Team:KIT-Kyoto/HumanPractice">policy & practices</a></li>
		+	<li class="menuimg"><a href="/Team:KIT-Kyoto/Achievement">Achievement</a></li>
	</ul>		</ul>
	<div class="active">		<div class="active">
Line 22:		Line 22:
	</div>		</div>
	<ul class="submenu">		<ul class="submenu">
-	<li><a href="/Team:KIT-Kyoto/Notebook/Labnote">LabNote</a></li>	+	<li class="category">
		+	<a href="javascript:void(0)">
		+	<font color="#143">Click Here To Enlarge</font>  LabNote
		+	</a>
		+	</li>
		+	<ul class="slidemenu">
		+	<li class="btn">
		+	<a href="">June
		+	</a>
		+	</li>
		+	<li class="btn">
		+	<a href="">July
		+	</a>
		+	</li>
		+	<li class="btn">
		+	<a href="">August
		+	</a>
		+	</li>
		+	<li class="btn">
		+	<a href="">September
		+	</a>
		+	</li>
		+	<li class="btn">
		+	<a href="">October
		+	</a>
		+	</li>
		+	</ul>
	<li><a href="/Team:KIT-Kyoto/Notebook/Protocol">Protocol</a></li>		<li><a href="/Team:KIT-Kyoto/Notebook/Protocol">Protocol</a></li>
	</ul>		</ul>
Line 30:		Line 56:
	<div id="container">		<div id="container">
	<div class="main-contents">		<div class="main-contents">
		+	<div id="ln">
		+	<div class="accordion">
	<h1>LabNote</h1>		<h1>LabNote</h1>
-	<h2>June</h2>	+	<h2><a href="javascript:void(0);">June</a></h2>
	<div class="scroll"></div>		<div class="scroll"></div>
		+	<div class="ac">
	<!-- タイトル -->		<!-- タイトル -->
	<h3>June 10, 2014</h3>		<h3>June 10, 2014</h3>
Line 44:		Line 73:
	<!--Miniprep-->		<!--Miniprep-->
	<strong>Miniprep</strong>		<strong>Miniprep</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 73:		Line 103:
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Miniprep終わり-->		<!--Miniprep終わり-->
Line 79:		Line 110:
	<!--Restriction Digest-->		<!--Restriction Digest-->
	<strong>Restriction Digest</strong>		<strong>Restriction Digest</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 91:		Line 123:
	</tr>		</tr>
	<tr>		<tr>
-	<td>BL21(DE3)PlysS</td>	+	<td>BL21(DE3)</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
-	<td>BL21(DE3)PlysS</td>	+	<td>BL21(DE3)</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
-	<td>BL21(DE3)PlysS</td>	+	<td>BL21(DE3)</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
-	<td>BL21(DE3)PlysS</td>	+	<td>BL21(DE3)</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
-	<td>CuMTSE62</td>	+	<td>CuMTS62</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
-	<td>BL21(DE3)PlysS</td>	+	<td>BL21(DE3)</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>Sal1, Not1</td>	+	<td><em>Sal</em>1, <em>Not</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
-	<td>BL21(DE3)PlysS</td>	+	<td>BL21(DE3)</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>Sal1, Not1</td>	+	<td><em>Sal</em>1, <em>Not</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
-	<td>BL21(DE3)PlysS</td>	+	<td>BL21(DE3)</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>Sal1, Not1</td>	+	<td><em>Sal</em>1, <em>Not</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
-	<td>BL21(DE3)PlysS</td>	+	<td>BL21(DE3)</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>Sal1, Not1</td>	+	<td><em>Sal</em>1, <em>Not</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Ristriction Digest-->		<!--Ristriction Digest-->
	<br>		<br>
Line 152:		Line 185:
	</p>		</p>
	<!--本文終わり-->		<!--本文終わり-->
-
	<!-- タイトル -->		<!-- タイトル -->
	<h3>June 11, 2014</h3>		<h3>June 11, 2014</h3>
Line 164:		Line 196:
	<!--AGE-->		<!--AGE-->
	<strong>AGE</strong>		<strong>AGE</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
	<th rowspan="2">Lane</th>		<th rowspan="2">Lane</th>
-	<th colspan="2">Sample/Marker</th>	+	<th colspan="3">Sample</th>
	<th rowspan="2">Enzyme</th>		<th rowspan="2">Enzyme</th>
	</tr>		</tr>
	<tr>		<tr>
		+	<th>Marker</th>
	<th>Vector</th>		<th>Vector</th>
	<th>Insert</th>		<th>Insert</th>
Line 177:		Line 211:
	<td>1</td>		<td>1</td>
	<td>λDNA-HindⅢ</td>		<td>λDNA-HindⅢ</td>
		+	<td></td>
	<td></td>		<td></td>
	<td></td>		<td></td>
Line 182:		Line 217:
	<tr>		<tr>
	<td>2</td>		<td>2</td>
		+	<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>N.D</td>	+	<td>Non Treated</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>3</td>		<td>3</td>
		+	<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>4</td>		<td>4</td>
		+	<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>Sal1, Not1</td>	+	<td><em>Sal</em>1, <em>Not</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>5</td>		<td>5</td>
		+	<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>N.D</td>	+	<td>Non Treated</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>6</td>		<td>6</td>
		+	<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>7</td>		<td>7</td>
		+	<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>Sal1, Not1</td>	+	<td><em>Sal</em>1, <em>Not</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>8</td>		<td>8</td>
		+	<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>N.D</td>	+	<td>Non Treated</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>9</td>		<td>9</td>
		+	<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>10</td>		<td>10</td>
		+	<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
-	<td>CuMTS62</td>	+	<td>CuMTS3</td>
-	<td>N.D</td>	+	<td><em>Sal</em>1, <em>Not</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>11</td>		<td>11</td>
		+	<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>EcoR1</td>	+	<td>Non Treated</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>12</td>		<td>12</td>
		+	<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>Sal1, Not1</td>	+	<td><em>Eco</em>R1</td>
		+	</tr>
		+	<tr>
		+	<td>13</td>
		+	<td></td>
		+	<td>pGEX6P-2</td>
		+	<td>CuMTS62</td>
		+	<td><em>Sal</em>1, <em>Not</em>1</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--AGE-->		<!--AGE-->
		+	<img src="/wiki/images/8/88/Kit_June11_c.jpg">
	<br>		<br>
	<br>		<br>
Line 264:		Line 319:
	<!--Transformation-->		<!--Transformation-->
	<strong>Transformation</strong>		<strong>Transformation</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 293:		Line 349:
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Transformation終わり-->		<!--Transformation終わり-->
	<br>		<br>
Line 308:		Line 365:
	<strong>Colony Isolation</strong><br>		<strong>Colony Isolation</strong><br>
	Select 6 colonies per plate from 4 plates from June 12.<br>		Select 6 colonies per plate from 4 plates from June 12.<br>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 344:		Line 402:
	</table>		</table>
		+	</div>
	<!--Colony Isolation-->		<!--Colony Isolation-->
	<br>		<br>
Line 362:		Line 421:
	<!--Miniprep-->		<!--Miniprep-->
	<strong>Miniprep</strong>		<strong>Miniprep</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 411:		Line 471:
	</table>		</table>
		+	</div>
	<!--Miniprep終わり-->		<!--Miniprep終わり-->
	<br>		<br>
	<!--Miniprep-->		<!--Miniprep-->
	<strong>Miniprep</strong>		<strong>Miniprep</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 445:		Line 507:
	</table>		</table>
		+	</div>
	<!--Miniprep終わり-->		<!--Miniprep終わり-->
	<br>		<br>
	<!--Restriction Digest-->		<!--Restriction Digest-->
	<strong>Restriction Digest</strong>		<strong>Restriction Digest</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 464:		Line 528:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 471:		Line 535:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 478:		Line 542:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 485:		Line 549:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 492:		Line 556:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 499:		Line 563:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 506:		Line 570:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 513:		Line 577:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 520:		Line 584:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 527:		Line 591:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 534:		Line 598:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 541:		Line 605:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Ristriction Digest-->		<!--Ristriction Digest-->
Line 550:		Line 615:
	<!--AGE-->		<!--AGE-->
	<strong>AGE</strong>		<strong>AGE</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
	<th rowspan="2">Lane</th>		<th rowspan="2">Lane</th>
-	<th colspan="2">Sample/Marker</th>	+	<th colspan="3">Sample</th>
	<th rowspan="2">Insert</th>		<th rowspan="2">Insert</th>
	<th rowspan="2">Enzyme</th>		<th rowspan="2">Enzyme</th>
Line 559:		Line 625:
	</tr>		</tr>
	<tr>		<tr>
		+	<th>Marker</th>
	<th>Host</th>		<th>Host</th>
	<th>Vector</th>		<th>Vector</th>
Line 565:		Line 632:
	<td>1</td>		<td>1</td>
	<td>1kb Ladder</td>		<td>1kb Ladder</td>
		+	<td></td>
	<td></td>		<td></td>
	<td></td>		<td></td>
Line 572:		Line 640:
	<td>2</td>		<td>2</td>
	<td>λDNA-HindⅢ</td>		<td>λDNA-HindⅢ</td>
		+	<td></td>
	<td></td>		<td></td>
	<td></td>		<td></td>
Line 578:		Line 647:
	<tr>		<tr>
	<td>3</td>		<td>3</td>
		+	<td></td>
	<td>1-a</td>		<td>1-a</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>4</td>		<td>4</td>
		+	<td></td>
	<td>1-b</td>		<td>1-b</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>5</td>		<td>5</td>
		+	<td></td>
	<td>2-a</td>		<td>2-a</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>6</td>		<td>6</td>
		+	<td></td>
	<td>2-b</td>		<td>2-b</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>7</td>		<td>7</td>
		+	<td></td>
	<td>3-a</td>		<td>3-a</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>8</td>		<td>8</td>
		+	<td></td>
	<td>3-b</td>		<td>3-b</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<td>9</td>		<td>9</td>
		+	<td></td>
	<td>6-a</td>		<td>6-a</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	<tr>		<tr>
	<td>10</td>		<td>10</td>
		+	<td></td>
	<td>6-b</td>		<td>6-b</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>11</td>		<td>11</td>
		+	<td></td>
	<td>BL21(DE3)</td>		<td>BL21(DE3)</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>12</td>		<td>12</td>
		+	<td></td>
	<td>BL21(DE3)</td>		<td>BL21(DE3)</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>13</td>		<td>13</td>
		+	<td></td>
	<td>BL21(DE3)</td>		<td>BL21(DE3)</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>14</td>		<td>14</td>
		+	<td></td>
	<td>BL21(DE3)</td>		<td>BL21(DE3)</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--AGE-->		<!--AGE-->
	<br>		<br>
-	Applied λDNA-HindⅢ to compare and contrast AGE of July 14 and June 11.	+	Apply λDNA-HindⅢ to compare and contrast AGE of July 14 and June 11.
	<br>		<br>
		+	<img src="/wiki/images/b/b6/Kit_June14_c.jpg">
	<br>		<br>
	</p>		</p>
Line 690:		Line 773:
	<!--Preculture-->		<!--Preculture-->
	<strong>Preculture</strong>		<strong>Preculture</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 724:		Line 808:
	</tr>		</tr>
	</table>		</table>
		+	</div>
		+	<!--Preculture終わり-->
		+	<br>
		+	<!--Main Culture-->
		+	<strong>Main Culture</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Host</th>
		+	<th colspan="2">Sample</th>
		+	<th rowspan="2">IPTG</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	</tr>
		+	<tr>
		+	<td>BL21(DE3)pLysS</td>
		+	<td>pGEX6P-2</td>
		+	<td>CuMTSE1</td>
		+	<td>Induced</td>
		+	</tr>
		+	<tr>
		+	<td>BL21(DE3)pLysS</td>
		+	<td>pGEX6P-2</td>
		+	<td>CuMTSE2</td>
		+	<td>Induced</td>
		+	</tr>
		+	<tr>
		+	<td>BL21(DE3)pLysS</td>
		+	<td>pGEX6P-2</td>
		+	<td>CuMTS3</td>
		+	<td>Induced</td>
		+	<tr>
		+	<td>BL21(DE3)pLysS</td>
		+	<td>pGEX6P-2</td>
		+	<td>CuMTS62</td>
		+	<td>Induced</td>
		+	</tr>
		+	<tr>
		+	<td>BL21(DE3)pLysS</td>
		+	<td>pGEX6P-2</td>
		+	<td></td>
		+	<td>Induced</td>
		+	<tr>
		+	<td>BL21(DE3)pLysS</td>
		+	<td>pGEX6P-2</td>
		+	<td>CuMTSE1</td>
		+	<td>Non-induced</td>
		+	</tr>
		+	<tr>
		+	<td>BL21(DE3)pLysS</td>
		+	<td>pGEX6P-2</td>
		+	<td>CuMTSE2</td>
		+	<td>Non-induced</td>
		+	</tr><tr>
		+	<td>BL21(DE3)pLysS</td>
		+	<td>pGEX6P-2</td>
		+	<td>CuMTS3</td>
		+	<td>Non-induced</td>
		+	</tr><tr>
		+	<td>BL21(DE3)pLysS</td>
		+	<td>pGEX6P-2</td>
		+	<td>CuMTS62</td>
		+	<td>Non-induced</td>
		+	</tr><tr>
		+	<td>BL21(DE3)pLysS</td>
		+	<td>pGEX6P-2</td>
		+	<td></td>
		+	<td>Non-induced</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<!--Main Culture終わり-->
		+	<br>
		+	</p>
		+	<!--本文終わり-->
		+	<br>
		+	<!-- タイトル -->
		+	<h3>June 18, 2014</h3>
		+	<!-- タイトル終わり -->
		+
		+	<!-- 本文開始 -->
		+	<p class="sentence">
		+	<!-- 実験者名 -->
		+	YAMAJI<br>
		+	<!-- 実験者名終わり -->
		+	<!--Preculture-->
		+	<strong>Preculture</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Host</th>
		+	<th colspan="2">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	</tr>
		+	<tr>
		+	<td>BL21(DE3)pLysS</td>
		+	<td>pGEX6P-2</td>
		+	<td>CuMTSE1</td>
		+	</tr>
		+	<td>BL21(DE3)pLysS</td>
		+	<td>pGEX6P-2</td>
		+	<td>CuMTSE2</td>
		+	</tr>
		+	</table>
		+	</div>
	<!--Preculture終わり-->		<!--Preculture終わり-->
	<br>		<br>
	<!--Main Culture-->		<!--Main Culture-->
	<strong>Main Culture</strong>		<strong>Main Culture</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 759:		Line 954:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td></td>		<td></td>
-	<td>Non-Induced</td>	+	<td>Non-induced</td>
	</tr>		</tr>
	<tr>		<tr>
Line 765:		Line 960:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>Non-Induced</td>	+	<td>Non-induced</td>
	<tr>		<tr>
	<td>BL21(DE3)pLysS</td>		<td>BL21(DE3)pLysS</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>Non-Induced</td>	+	<td>Non-induced</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Main Culture終わり-->		<!--Main Culture終わり-->
	<br>		<br>
-	<!--Protein Extraction (<em>E.coli</em>)-->	+	<!--Protein Extraction (<em>E. coli</em>)-->
-	<strong>Protein Extraction (<em>E.coli</em>)</strong>	+	<strong>Protein Extraction (<em>E. coli</em>)</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 808:		Line 1,005:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td></td>		<td></td>
-	<td>Non-Induced</td>	+	<td>Non-induced</td>
	</tr>		</tr>
	<tr>		<tr>
Line 814:		Line 1,011:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>Non-Induced</td>	+	<td>Non-induced</td>
	<tr>		<tr>
	<td>BL21(DE3)pLysS</td>		<td>BL21(DE3)pLysS</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>Non-Induced</td>	+	<td>Non-induced</td>
	</tr>		</tr>
	</table>		</table>
-	<!--Protein Extraction (<em>E.coli</em>)終わり-->	+	</div>
		+	<!--Protein Extraction (<em>E. coli</em>)終わり-->
	<br>		<br>
	<!--Preparations for SDS-PAGE-->		<!--Preparations for SDS-PAGE-->
Line 830:		Line 1,028:
	<!--Preparations for SDS-PAGE終わり-->		<!--Preparations for SDS-PAGE終わり-->
	<br>		<br>
		+	<img src="/wiki/images/0/09/Kit_June18_c.jpg">
	<br>		<br>
	<br>		<br>
Line 846:		Line 1,045:
	<!--SDS-PAGE-->		<!--SDS-PAGE-->
	<strong>SDS-PAGE</strong>		<strong>SDS-PAGE</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 877:		Line 1,077:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td></td>		<td></td>
-	<td>Non-Induced</td>	+	<td>Non-induced</td>
	</tr>		</tr>
	<tr>		<tr>
Line 883:		Line 1,083:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>Non-Induced</td>	+	<td>Non-induced</td>
	<tr>		<tr>
	<td>BL21(DE3)pLysS</td>		<td>BL21(DE3)pLysS</td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>Non-Induced</td>	+	<td>Non-induced</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--SDS-PAGE終わり-->		<!--SDS-PAGE終わり-->
Line 909:		Line 1,110:
	<!--Miniprep-->		<!--Miniprep-->
	<strong>Miniprep</strong>		<strong>Miniprep</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 929:		Line 1,131:
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Mineprep終わり-->		<!--Mineprep終わり-->
	<br>		<br>
	<!--PCR-->		<!--PCR-->
	<strong>PCR</strong>		<strong>PCR</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 946:		Line 1,150:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
-	<td>CuMTSE2</td>	+	<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
-	<td>CuMTSE1</td>	+	<td>CuMTSE2</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	<tr>		<tr>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--PCR終わり-->		<!--PCR終わり-->
	<br>		<br>
	<!--AGE-->		<!--AGE-->
	<strong>AGE</strong>		<strong>AGE</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
	<th rowspan="2">Lane</th>		<th rowspan="2">Lane</th>
-	<th colspan="2">Sample/Marker</th>	+	<th colspan="3">Sample</th>
	<th rowspan="2">Insert</th>		<th rowspan="2">Insert</th>
	<th rowspan="2">Primer</th>		<th rowspan="2">Primer</th>
	</tr>		</tr>
	<tr>		<tr>
		+	<th>Marker</th>
	<th>Host</th>		<th>Host</th>
	<th>Vector</th>		<th>Vector</th>
Line 985:		Line 1,192:
	<td>1</td>		<td>1</td>
	<td>1kb Ladder</td>		<td>1kb Ladder</td>
		+	<td></td>
	<td></td>		<td></td>
	<td></td>		<td></td>
Line 991:		Line 1,199:
	<tr>		<tr>
	<td>2</td>		<td>2</td>
		+	<td></td>
	<td>BY4247</td>		<td>BY4247</td>
	<td>p427-TEF</td>		<td>p427-TEF</td>
Line 998:		Line 1,207:
	<tr>		<tr>
	<td>3</td>		<td>3</td>
		+	<td></td>
	<td></td>		<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<tr>		<tr>
	<td>4</td>		<td>4</td>
		+	<td></td>
	<td></td>		<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
-	<td>CuMTSE2</td>	+	<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>5</td>		<td>5</td>
		+	<td></td>
	<td></td>		<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
-	<td>CuMTSE1</td>	+	<td>CuMTSE2</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>6</td>		<td>6</td>
		+	<td></td>
	<td></td>		<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--AGE終わり-->		<!--AGE終わり-->
	<br>		<br>
		+	<img src="/wiki/images/2/29/Kit_June21_c.jpg">
	<br>		<br>
	</p>		</p>
Line 1,042:		Line 1,257:
	<!--Preculture-->		<!--Preculture-->
	<strong>Preculture</strong>		<strong>Preculture</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,066:		Line 1,282:
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
	</table>		</table>
		+	</div>
	<!--Preculture終わり-->		<!--Preculture終わり-->
	<br>		<br>
Line 1,082:		Line 1,299:
	<!-- 実験者名終わり -->		<!-- 実験者名終わり -->
	<br>		<br>
-	Cultivated following samples in Big Scale at 30℃ overnight in shaken culture.	+	Cultivate following samples in Big Scale at 30℃ overnight in shaken culture.
	<br>		<br>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,094:		Line 1,312:
	</tr>		</tr>
	<tr>		<tr>
-	<td colspan="3">BL21(DE3)pLysS</td>	+	<td>BL21(DE3)pLysS</td>
		+	<td></td>
		+	<td></td>
	</tr>		</tr>
	<tr>		<tr>
Line 1,107:		Line 1,327:
	<tr>		<tr>
	</table>		</table>
		+	</div>
	<br>		<br>
-	<!--DNA Refinement1-->	+	<!--DNA Refinement 1-->
-	<strong>DNA Refinement1</strong>	+	<strong>DNA Refinement 1</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,122:		Line 1,344:
	<tr>		<tr>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
-	<td>CuMTSE2</td>	+	<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</table>		</table>
-	<!--DNA Refinement1終わり-->	+	</div>
		+	<!--DNA Refinement 1終わり-->
	<br>		<br>
Line 1,134:		Line 1,357:
	<!--Restriction Digest-->		<!--Restriction Digest-->
	<strong>Restriction Digest</strong>		<strong>Restriction Digest</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,148:		Line 1,372:
	<td></td>		<td></td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
	<td></td>		<td></td>
-	<td>CuMTSE2</td>	+	<td>CuMTSE1</td>
-	<td>P-F2, P-R	+	<td>pGEX6P-2F2, pGEX6P-2R
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 1,163:		Line 1,387:
	<td></td>		<td></td>
	<td></td>		<td></td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Ristriction Digest-->		<!--Ristriction Digest-->
	<br>		<br>
-	<!--DNA Refinement2-->	+	<!--DNA Refinement 2-->
-	<strong>DNA Refinement2</strong>	+	<strong>DNA Refinement 2</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,186:		Line 1,412:
	<td></td>		<td></td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td></td>		<td></td>
-	<td>CuMTSE2</td>	+	<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 1,199:		Line 1,425:
	<td></td>		<td></td>
	<td></td>		<td></td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	</table>		</table>
-	<!--DNA Refinement2-->	+	</div>
		+	<!--DNA Refinement 2-->
	<br>		<br>
Line 1,208:		Line 1,435:
	<!--Main Culture-->		<!--Main Culture-->
	<strong>Main Culture</strong>		<strong>Main Culture</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,240:		Line 1,468:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td></td>		<td></td>
-	<td>Non-Induced</td>	+	<td>Non-induced</td>
	</tr>		</tr>
	<tr>		<tr>
Line 1,246:		Line 1,474:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>Non-Induced</td>	+	<td>Non-induced</td>
	</tr>		</tr>
	<tr>		<tr>
Line 1,252:		Line 1,480:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>Non-Induced</td>	+	<td>Non-induced</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Main Culture-->		<!--Main Culture-->
-		+	<img src="/wiki/images/1/1f/Kit_June25_c.jpg">
	<br>		<br>
	<!--Ligation-->		<!--Ligation-->
	<strong>Ligation</strong>		<strong>Ligation</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,273:		Line 1,503:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	DNA ligase: DNA Ligation Kit ‹Mighty Mix› (TAKARA BIO INC.)		DNA ligase: DNA Ligation Kit ‹Mighty Mix› (TAKARA BIO INC.)
	<!--Ligation-->		<!--Ligation-->
Line 1,298:		Line 1,529:
	<!--Transformation-->		<!--Transformation-->
	<strong>Transformation</strong>		<strong>Transformation</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,312:		Line 1,544:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 1,318:		Line 1,550:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 1,324:		Line 1,556:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 1,330:		Line 1,562:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Transformation-->		<!--Transformation-->
	<br>		<br>
Line 1,353:		Line 1,586:
	Isolate each colony from the plates which were inoculated on June 25.		Isolate each colony from the plates which were inoculated on June 25.
	<br>		<br>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,368:		Line 1,602:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<td>1～4</td>		<td>1～4</td>
	</tr>		</tr>
Line 1,375:		Line 1,609:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	<td>a～h</td>		<td>a～h</td>
	</tr>		</tr>
Line 1,382:		Line 1,616:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	<td>ア～タ</td>		<td>ア～タ</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Colony Isolation-->		<!--Colony Isolation-->
	<br>		<br>
Line 1,401:		Line 1,636:
	<!--Colony Sweep-->		<!--Colony Sweep-->
	<strong>Colony Sweep</strong>		<strong>Colony Sweep</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,443:		Line 1,679:
	<tr>		<tr>
	<td>6</td>		<td>6</td>
-	<td>A</td>	+	<td>a</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
Line 1,449:		Line 1,685:
	<tr>		<tr>
	<td>7</td>		<td>7</td>
-	<td>B</td>	+	<td>b</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
Line 1,455:		Line 1,691:
	<tr>		<tr>
	<td>8</td>		<td>8</td>
-	<td>C</td>	+	<td>c</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
Line 1,461:		Line 1,697:
	<tr>		<tr>
	<td>9</td>		<td>9</td>
-	<td>D</td>	+	<td>d</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
Line 1,467:		Line 1,703:
	<tr>		<tr>
	<td>10</td>		<td>10</td>
-	<td>E</td>	+	<td>e</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
Line 1,473:		Line 1,709:
	<tr>		<tr>
	<td>11</td>		<td>11</td>
-	<td>F</td>	+	<td>f</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
Line 1,479:		Line 1,715:
	<tr>		<tr>
	<td>12</td>		<td>12</td>
-	<td>G</td>	+	<td>g</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
Line 1,485:		Line 1,721:
	<tr>		<tr>
	<td>13</td>		<td>13</td>
-	<td>H</td>	+	<td>h</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Colony Sweep-->		<!--Colony Sweep-->
		+	<img src="/wiki/images/4/4d/Kit_June27-1_c.jpg">
	<br>		<br>
	<!--Colony Sweep-->		<!--Colony Sweep-->
	<strong>Colony Sweep</strong>		<strong>Colony Sweep</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,608:		Line 1,847:
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Colony Sweep-->		<!--Colony Sweep-->
		+	<img src="/wiki/images/4/46/Kit_June27-2_c.jpg">
	<br>		<br>
	Identified targeted genes in 4, c, h, イ, カ, セ		Identified targeted genes in 4, c, h, イ, カ, セ
Line 1,617:		Line 1,858:
	<!--Miniprep-->		<!--Miniprep-->
	<strong>Miniprep</strong>		<strong>Miniprep</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,642:		Line 1,884:
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Miniprep終わり-->		<!--Miniprep終わり-->
-
	<br>		<br>
Line 1,649:		Line 1,891:
	<!--Restriction Digest-->		<!--Restriction Digest-->
	<strong>Restriction Digest</strong>		<strong>Restriction Digest</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,660:		Line 1,903:
	<tr>		<tr>
	<td>4</td>		<td>4</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>C</td>		<td>C</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>H</td>		<td>H</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	<tr>		<tr>
	<td>イ</td>		<td>イ</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>カ</td>		<td>カ</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Restriction Digest終わり-->		<!--Restriction Digest終わり-->
Line 1,689:		Line 1,933:
	<!--AGE-->		<!--AGE-->
	<strong>AGE</strong>		<strong>AGE</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
	<th rowspan="2">Lane</th>		<th rowspan="2">Lane</th>
-	<th colspan="2">Sample/Marker</th>	+	<th colspan="3">Sample</th>
	<th rowspan="2">Primer</th>		<th rowspan="2">Primer</th>
	<th rowspan="2">Enzyme</th>		<th rowspan="2">Enzyme</th>
	</tr>		</tr>
	<tr>		<tr>
		+	<th>Marker</th>
	<th>Host</th>		<th>Host</th>
	<th>Insert</th>		<th>Insert</th>
Line 1,702:		Line 1,948:
	<tr>		<tr>
	<td>1</td>		<td>1</td>
-	<td>ikb Ladder</td>	+	<td>1kb Ladder</td>
		+	<td></td>
		+	<td></td>
	<td></td>		<td></td>
	<td></td>		<td></td>
	</tr>		</tr>
	<tr>		<tr>
-	<td colspan="2">2</td>	+	<td>2</td>
		+	<td></td>
		+	<td></td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<td></td>		<td></td>
	</tr>		</tr>
	<tr>		<tr>
	<td>3</td>		<td>3</td>
		+	<td></td>
	<td>4</td>		<td>4</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>4</td>		<td>4</td>
		+	<td></td>
	<td>4</td>		<td>4</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>5</td>		<td>5</td>
		+	<td></td>
	<td>4</td>		<td>4</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
-	<td>N.D</td>	+	<td>Non Treated</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>6</td>		<td>6</td>
		+	<td></td>
	<td></td>		<td></td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	<td></td>		<td></td>
	</tr>		</tr>
	<tr>		<tr>
	<td>7</td>		<td>7</td>
		+	<td></td>
	<td>c</td>		<td>c</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>8</td>		<td>8</td>
		+	<td></td>
	<td>c</td>		<td>c</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>9</td>		<td>9</td>
		+	<td></td>
	<td>c</td>		<td>c</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
-	<td>N.D</td>	+	<td>Non Treated</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>10</td>		<td>10</td>
		+	<td></td>
	<td>h</td>		<td>h</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>11</td>		<td>11</td>
		+	<td></td>
	<td>h</td>		<td>h</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>12</td>		<td>12</td>
		+	<td></td>
	<td>h</td>		<td>h</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
-	<td>N.D</td>	+	<td>Non Treated</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--AGE-->		<!--AGE-->
		+	<img src="/wiki/images/9/95/June27-3_c.jpg">
	<br>		<br>
	<!--Transformation (electroportation)-->		<!--Transformation (electroportation)-->
	<strong>Transformation (electroportation)</strong>		<strong>Transformation (electroportation)</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,802:		Line 2,065:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 1,808:		Line 2,071:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R<td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Transformation (electroportation)-->		<!--Transformation (electroportation)-->
Line 1,832:		Line 2,096:
	Divide each of 2 plates into 8 sections and inoculate following samples on them.		Divide each of 2 plates into 8 sections and inoculate following samples on them.
	<br>		<br>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,847:		Line 2,112:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<td>A～H</td>		<td>A～H</td>
	</tr>		</tr>
Line 1,854:		Line 2,119:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	<td>一～ハ</td>		<td>一～ハ</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	Cultivate 17 samples in a small scale at 30℃ for 6 hours in shaken culture.		Cultivate 17 samples in a small scale at 30℃ for 6 hours in shaken culture.
	<!--Inoculation-->		<!--Inoculation-->
Line 1,867:		Line 2,133:
	</p>		</p>
	<!--本文終わり-->		<!--本文終わり-->
		+	</div>
		+	</div>
-	<h2>July</h2>	+	<div class="accordion">
		+	<h2><a href="javascript:void(0);">July</a></h2>
	<div class="scroll"></div>		<div class="scroll"></div>
		+	<div class="ac">
Line 1,881:		Line 2,151:
	KOBAYASHI<br>		KOBAYASHI<br>
	<!-- 実験者名終わり -->		<!-- 実験者名終わり -->
-	<!--Protein Extraction (<em>Saccharomyces cerevisiae</em>)-->	+	<!--Protein Extraction (<em>S. cerevisiae</em>)-->
-	<strong>Protein Extraction (<em>Saccharomyces cerevisiae</em>)</strong>	+	<strong>Protein Extraction (<em>S. cerevisiae</em>)</strong>
-	<br>	+	<div class="m_table">
-	Divide each of 2 plates into 8 sections and inoculate following samples on them.	+
-	<br>	+
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,900:		Line 2,168:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 1,906:		Line 2,174:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	</table>		</table>
-	<!--Protein Extraction (<em>Saccharomyces cerevisiae</em>)-->	+	</div>
		+	<!--Protein Extraction (<em>S. cerevisiae</em>)-->
	<br>		<br>
Line 1,926:		Line 2,195:
	<strong>Miniprep</strong>		<strong>Miniprep</strong>
	<br>		<br>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,938:		Line 2,208:
	<tr>		<tr>
	<td>BY4247</td>		<td>BY4247</td>
-	<td>p426-TEF</td>	+	<td>p427-TEF</td>
	<td></td>		<td></td>
	<td></td>		<td></td>
Line 1,946:		Line 2,216:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 1,952:		Line 2,222:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Miniprep-->		<!--Miniprep-->
Line 1,961:		Line 2,232:
	<!--Restriction Digest-->		<!--Restriction Digest-->
	<strong>Restriction Digest</strong>		<strong>Restriction Digest</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 1,976:		Line 2,248:
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
	<td></td>		<td></td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 1,982:		Line 2,254:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 1,989:		Line 2,261:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 1,997:		Line 2,269:
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
	<td></td>		<td></td>
-	<td>Sac1</td>	+	<td><em>Sac</em>1, <em>Kpn</em>1</td>
	<td>Buffer L</td>		<td>Buffer L</td>
	</tr>		</tr>
Line 2,003:		Line 2,275:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Sac1</td>	+	<td><em>Sac</em>1, <em>Kpn</em>1</td>
	<td>Buffer L</td>		<td>Buffer L</td>
	</tr>		</tr>
Line 2,010:		Line 2,282:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2,P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
-	<td>Sac1</td>	+	<td><em>Sac</em>1, <em>Kpn</em>1</td>
	<td>Buffer L</td>		<td>Buffer L</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<!--Restriction Digest-->
		+
		+	<br>
		+
		+	<!--AGE-->
		+	<strong>AGE</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Lane</th>
		+	<th colspan="4">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	</tr>
		+	<tr>
		+	<th>Marker</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>1</td>
		+	<td></td>
		+	<td>p427-TEF</td>
		+	<td></td>
		+	<td></td>
		+	<td>Non Treated</td>
		+	</tr>
		+	<tr>
		+	<td>2</td>
		+	<td></td>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE1</td>
		+	<td>pGEX6P-2F1, pGEX6P-2R</td>
		+	<td>Non Treated</td>
		+	</tr>
		+	<tr>
		+	<td>3</td>
		+	<td></td>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE1</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	<td>Non Treated</td>
		+	</tr>
		+	<tr>
		+	<td>4</td>
		+	<td>1kb Ladder</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>5</td>
		+	<td></td>
		+	<td>p427-TEF</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Eco</em>R1</td>
		+	</tr>
		+	<tr>
		+	<td>6</td>
		+	<td></td>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE1</td>
		+	<td>pGEX6P-2F1, pGEX6P-2R</td>
		+	<td><em>Eco</em>R1</td>
		+	</tr>
		+	<tr>
		+	<td>7</td>
		+	<td></td>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE1</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	<td><em>Eco</em>R1</td>
		+	</tr>
		+	<tr>
		+	<td>8</td>
		+	<td>1kb Ladder</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>9</td>
		+	<td></td>
		+	<td>p427-TEF</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Kpn</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>10</td>
		+	<td></td>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE1</td>
		+	<td>pGEX6P-2F1, pGEX6P-2R</td>
		+	<td><em>Kpn</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>11</td>
		+	<td></td>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE1</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	<td><em>Kpn</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>12</td>
		+	<td>1kb Ladder</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>13</td>
		+	<td></td>
		+	<td>p427-TEF</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Sac</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>14</td>
		+	<td></td>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE1</td>
		+	<td>pGEX6P-2F1, pGEX6P-2R</td>
		+	<td><em>Sac</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>15</td>
		+	<td></td>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE1</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	<td><em>Sac</em>1</td>
		+	</tr>
		+	<tr>
		+	</table>
		+	</div>
		+	<!--AGE-->
		+	<img src="/wiki/images/8/82/Kit_July2-1_c.jpg">
		+	<br>
		+
		+	<!--Restriction Digest-->
		+	<strong>Restriction Digest</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="3">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	<th rowspan="2">Buffer</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE1</td>
		+	<td></td>
		+	<td><em>Eco</em>R1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE1</td>
		+	<td>pGEX6P-2F1, pGEX6P-2R</td>
		+	<td><em>Eco</em>R1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE1</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	<td><em>Eco</em>R1</td>
		+	<td>Buffer H</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,018:		Line 2,473:
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
	<td></td>		<td></td>
-	<td>Kpn1</td>	+	<td><em>Sac</em>1</td>
	<td>Buffer L</td>		<td>Buffer L</td>
	</tr>		</tr>
Line 2,024:		Line 2,479:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Kpn1</td>	+	<td><em>Sac</em>1</td>
	<td>Buffer L</td>		<td>Buffer L</td>
	</tr>		</tr>
Line 2,031:		Line 2,486:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
-	<td>Kpn1</td>	+	<td><em>Sac</em>1</td>
	<td>Buffer L</td>		<td>Buffer L</td>
	</tr>		</tr>
Line 2,039:		Line 2,494:
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
	<td></td>		<td></td>
-	<td>Spe1, Xho1</td>	+	<td><em>Kpn</em>1</td>
		+	<td>Buffer L</td>
		+	</tr>
		+	<tr>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE1</td>
		+	<td>pGEX6P-2F1, pGEX6P-2R</td>
		+	<td><em>Kpn</em>1</td>
		+	<td>Buffer L</td>
		+	</tr>
		+	<tr>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE1</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	<td><em>Kpn</em>1</td>
		+	<td>Buffer L</td>
		+	</tr>
		+	<tr>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE1</td>
		+	<td></td>
		+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 2,045:		Line 2,521:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1,Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
Line 2,052:		Line 2,528:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Buffer H</td>		<td>Buffer H</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Restriction Digest-->		<!--Restriction Digest-->
Line 2,063:		Line 2,540:
	<!--AGE-->		<!--AGE-->
	<strong>AGE</strong>		<strong>AGE</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,070:		Line 2,548:
	</tr>		</tr>
	<tr>		<tr>
-	<th></th>	+	<th>Marker</th>
	<th>Vector</th>		<th>Vector</th>
	<th>Insert</th>		<th>Insert</th>
Line 2,089:		Line 2,567:
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
	<td></td>		<td></td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,096:		Line 2,574:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,104:		Line 2,582:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
-	<td>EcoR1</td>	+	<td><em>Eco</em>R1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,113:		Line 2,591:
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
	<td></td>		<td></td>
-	<td>Kpn1</td>	+	<td><em>Kpn</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,120:		Line 2,598:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Kpn1</td>	+	<td><em>Kpn</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,128:		Line 2,606:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
-	<td>Kpn1</td>	+	<td><em>Kpn</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,137:		Line 2,615:
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
	<td></td>		<td></td>
-	<td>Sac1</td>	+	<td><em>Sac</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,144:		Line 2,622:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Sac1</td>	+	<td><em>Sac</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,152:		Line 2,630:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
-	<td>Sac1</td>	+	<td><em>Sac</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,161:		Line 2,639:
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
	<td></td>		<td></td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,168:		Line 2,646:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,176:		Line 2,654:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--AGE-->		<!--AGE-->
-		+	<img src="/wiki/images/c/c1/Kit_July2-2_c.jpg">
	<br>		<br>
	<br>		<br>
Line 2,197:		Line 2,676:
	<strong>PCR</strong>		<strong>PCR</strong>
	<br>		<br>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,210:		Line 2,690:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
Line 2,216:		Line 2,696:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
Line 2,222:		Line 2,702:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
Line 2,228:		Line 2,708:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
Line 2,234:		Line 2,714:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
Line 2,240:		Line 2,720:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--PCR-->		<!--PCR-->
	<br>		<br>
-	<!--Protein Extraction (<em>Saccharomyces cerevisiae</em>)-->	+	<!--Protein Extraction (<em>S. cerevisiae</em>)-->
-	<strong>Protein Extraction (<em>Saccharomyces cerevisiae</em>)</strong>	+	<strong>Protein Extraction (<em>S. cerevisiae</em>)</strong>
	<br>		<br>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,268:		Line 2,750:
	</tr>		</tr>
	<tr>		<tr>
-	<td>六</td>	+	<td>A</td>
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
-	<td>A</td>	+	<td>六</td>
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	</table>		</table>
-	<!--Protein Extraction (<em>Saccharomyces cerevisiae</em>)-->	+	</div>
		+	<!--Protein Extraction (<em>S. cerevisiae</em>)-->
	<br>		<br>
Line 2,296:		Line 2,779:
	<!--AGE-->		<!--AGE-->
	<strong>AGE</strong>		<strong>AGE</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
	<th rowspan="2">Lane</th>		<th rowspan="2">Lane</th>
-	<th colspan="4">Sample</th>	+	<th colspan="4">Sample/Marker</th>
	</tr>		</tr>
	<tr>		<tr>
-	<th>Host</th>	+	<th>Marker</th>
	<th>Vector</th>		<th>Vector</th>
	<th>Insert</th>		<th>Insert</th>
Line 2,319:		Line 2,803:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,325:		Line 2,809:
	<td></td>		<td></td>
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
-	<td>1kb Ladder+K83:N91</td>	+	<td>CuMTSE2</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,333:		Line 2,817:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,340:		Line 2,824:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,347:		Line 2,831:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,354:		Line 2,838:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--AGE-->		<!--AGE-->
-		+	<img src="/wiki/images/9/94/Kit_July4-1_c.jpg">
	<br>		<br>
Line 2,364:		Line 2,849:
	<strong>PCR</strong>		<strong>PCR</strong>
	<br>		<br>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,377:		Line 2,863:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
Line 2,383:		Line 2,869:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
Line 2,389:		Line 2,875:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
Line 2,395:		Line 2,881:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
Line 2,401:		Line 2,887:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
Line 2,407:		Line 2,893:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	<td>KOD-FX-NEO</td>		<td>KOD-FX-NEO</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--PCR-->		<!--PCR-->
Line 2,417:		Line 2,904:
	<!--AGE-->		<!--AGE-->
	<strong>AGE</strong>		<strong>AGE</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,423:		Line 2,911:
	</tr>		</tr>
	<tr>		<tr>
-	<th></th>	+	<th>Marker</th>
	<th>Vector</th>		<th>Vector</th>
	<th>Insert</th>		<th>Insert</th>
Line 2,440:		Line 2,928:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,447:		Line 2,935:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>1kb Ladder+K83:N91</td>		<td>1kb Ladder+K83:N91</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,454:		Line 2,942:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,461:		Line 2,949:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,468:		Line 2,956:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,475:		Line 2,963:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--AGE-->		<!--AGE-->
-		+	<img src="/wiki/images/d/d4/Kit_July4-2_c.jpg">
	<br>		<br>
	<br>		<br>
Line 2,495:		Line 2,984:
	<strong>SDS-PAGE</strong>		<strong>SDS-PAGE</strong>
	<br>		<br>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,515:		Line 3,005:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,521:		Line 3,011:
	<td>pGEX6P-2</td>		<td>pGEX6P-2</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	<tr>		<tr>
	<td>BY4247</td>		<td>BY4247</td>
Line 2,529:		Line 3,019:
	</tr>		</tr>
	<tr>		<tr>
-	<td>六</td>	+	<td>A</td>
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE1</td>		<td>CuMTSE1</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
-	<td>A</td>	+	<td>六</td>
	<td>p427-TEF</td>		<td>p427-TEF</td>
-	<td>CuMTSE2</td>	+	<td>CuMTSE1</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--SDS-PAGE-->		<!--SDS-PAGE-->
Line 2,556:		Line 3,047:
	SOGA<br>		SOGA<br>
	<!-- 実験者名終わり -->		<!-- 実験者名終わり -->
-	<!--DNA Refinement1-->	+	<!--DNA Refinement 1-->
-	<strong>DNA Refinement1</strong>	+	<strong>DNA Refinement 1</strong>
	<br>		<br>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,568:		Line 3,060:
	</tr>		</tr>
	<tr>		<tr>
-	<td>CuMTSE1</td>	+	<td>CuMTSE2</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
	<tr>		<tr>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	</tr>		</tr>
	</table>		</table>
-	<!--DNA Refinement1-->	+	</div>
		+	<!--DNA Refinement 1-->
	<br>		<br>
Line 2,589:		Line 3,082:
	<!--Restriction Digest-->		<!--Restriction Digest-->
	<strong>Restriction Digest</strong>		<strong>Restriction Digest</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,603:		Line 3,097:
	<td></td>		<td></td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Fast digest buffer</td>		<td>Fast digest buffer</td>
	</tr>		</tr>
Line 2,610:		Line 3,104:
	<td></td>		<td></td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Fast digest buffer</td>		<td>Fast digest buffer</td>
	</tr>		</tr>
Line 2,617:		Line 3,111:
	<td></td>		<td></td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Fast digest buffer</td>		<td>Fast digest buffer</td>
	</tr>		</tr>
	<td></td>		<td></td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Fast digest buffer</td>		<td>Fast digest buffer</td>
	</tr>		</tr>
Line 2,630:		Line 3,124:
	<td></td>		<td></td>
	<td></td>		<td></td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Fast digest buffer</td>		<td>Fast digest buffer</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Ristriction Digest-->		<!--Ristriction Digest-->
	<br>		<br>
-	<!--DNA Refinement2-->	+	<!--DNA Refinement 2-->
-	<strong>DNA Refinement2</strong>	+	<strong>DNA Refinement 2</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,651:		Line 3,147:
	<tr>		<tr>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	</table>		</table>
-	<!--DNA Refinement2-->	+	</div>
		+	<!--DNA Refinement 2-->
	<br>		<br>
Line 2,666:		Line 3,163:
	<!--Ligation-->		<!--Ligation-->
	<strong>Ligation</strong>		<strong>Ligation</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,679:		Line 3,177:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<br>		<br>
	DNA ligase: DNA Ligation Kit ‹Mighty Mix› (TAKARA BIO INC.)		DNA ligase: DNA Ligation Kit ‹Mighty Mix› (TAKARA BIO INC.)
	<br>		<br>
-	<!--DNA Refinement2-->	+	<!--DNA Refinement 2-->
	<br>		<br>
-	<!--Transformation (<em>E.coli</em>)-->	+	<!--Transformation (<em>E. coli</em>)-->
-	<strong>Transformation (<em>E.coli</em>)</strong>	+	<strong>Transformation (<em>E. coli</em>)</strong>
	<br>		<br>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,714:		Line 3,214:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,721:		Line 3,221:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,728:		Line 3,228:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
Line 2,735:		Line 3,235:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	</table>		</table>
-	<!--Transformation (<em>E.coli</em>)-->	+	</div>
		+	<!--Transformation (<em>E. coli</em>)-->
	</p>		</p>
	<!--本文終わり-->		<!--本文終わり-->
Line 2,760:		Line 3,261:
	Isolate each colony from the plates which were inoculated on July 6.		Isolate each colony from the plates which were inoculated on July 6.
	<br>		<br>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,775:		Line 3,277:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<td>1～16</td>		<td>1～16</td>
	</tr>		</tr>
Line 2,782:		Line 3,284:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<td>ア～エ</td>		<td>ア～エ</td>
	</tr>		</tr>
Line 2,789:		Line 3,291:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
	<td>a～p</td>		<td>a～p</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Colony Isolation-->		<!--Colony Isolation-->
	<br>		<br>
-	<!--Transformation (<em>E.coli</em>)-->	+	<!--Transformation (<em>E. coli</em>)-->
-	<strong>Transformation (<em>E.coli</em>)</strong>	+	<strong>Transformation (<em>E. coli</em>)</strong>
	<br>		<br>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,815:		Line 3,319:
	<td>p427-TEF</td>		<td>p427-TEF</td>
	<td>CuMTSE2</td>		<td>CuMTSE2</td>
-	<td>P-F2, P-R</td>	+	<td>pGEX6P-2F2, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	</table>		</table>
-	<!--Transformation (<em>E.coli</em>)-->	+	</div>
		+	<!--Transformation (<em>E. coli</em>)-->
	<br>		<br>
Line 2,835:		Line 3,340:
	<!--Colony Sweep-->		<!--Colony Sweep-->
	<strong>Colony Sweep</strong>		<strong>Colony Sweep</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,930:		Line 3,436:
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Colony Sweep-->		<!--Colony Sweep-->
-		+	<img src="/wiki/images/e/e6/Kit_July8-1_c.jpg">
	<br>		<br>
	<!--Colony Sweep-->		<!--Colony Sweep-->
	<strong>Colony Sweep</strong>		<strong>Colony Sweep</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 2,971:		Line 3,479:
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Colony Sweep-->		<!--Colony Sweep-->
-		+	<img src="/wiki/images/c/c1/Kit_July8-2_c.jpg">
	<br>		<br>
	<!--Colony Sweep-->		<!--Colony Sweep-->
	<strong>Colony Sweep</strong>		<strong>Colony Sweep</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 3,072:		Line 3,582:
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Colony Sweep-->		<!--Colony Sweep-->
-		+	<img src="/wiki/images/c/c3/Kit_July8-3_c.jpg">
	<br>		<br>
	<br>		<br>
Line 3,088:		Line 3,599:
	<!--Main Culture-->		<!--Main Culture-->
	<strong>Main Culture</strong>		<strong>Main Culture</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 3,096:		Line 3,608:
	</tr>		</tr>
	<tr>		<tr>
-	<td>D</td>	+	<td>d</td>
	</tr>		</tr>
	<tr>		<tr>
-	<td>H</td>	+	<td>h</td>
	</tr>		</tr>
	<tr>		<tr>
-	<td>L</td>	+	<td>l</td>
	</tr>		</tr>
	<tr>		<tr>
Line 3,114:		Line 3,626:
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Main Culture-->		<!--Main Culture-->
Line 3,120:		Line 3,633:
	<!--Mini Prep-->		<!--Mini Prep-->
	<strong>Mini Prep</strong>		<strong>Mini Prep</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 3,146:		Line 3,660:
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Mini Prep-->		<!--Mini Prep-->
Line 3,152:		Line 3,667:
	<!--Restriction Digest-->		<!--Restriction Digest-->
	<strong>Restriction Digest</strong>		<strong>Restriction Digest</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 3,166:		Line 3,682:
	<td>p427-TEF(d)</td>		<td>p427-TEF(d)</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Fast digest buffer</td>		<td>Fast digest buffer</td>
	</tr>		</tr>
Line 3,174:		Line 3,690:
	<td>p427-TEF(h)</td>		<td>p427-TEF(h)</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Fast digest buffer</td>		<td>Fast digest buffer</td>
	</tr>		</tr>
Line 3,182:		Line 3,698:
	<td>p427-TEF(l)</td>		<td>p427-TEF(l)</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Fast digest buffer</td>		<td>Fast digest buffer</td>
	</tr>		</tr>
Line 3,189:		Line 3,705:
	<td>p427-TEF(ア)</td>		<td>p427-TEF(ア)</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Fast digest buffer</td>		<td>Fast digest buffer</td>
	</tr>		</tr>
Line 3,197:		Line 3,713:
	<td>p427-TEF(イ)</td>		<td>p427-TEF(イ)</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Fast digest buffer</td>		<td>Fast digest buffer</td>
	</tr>		</tr>
Line 3,205:		Line 3,721:
	<td>p427-TEF(エ)</td>		<td>p427-TEF(エ)</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	<td>Fast digest buffer</td>		<td>Fast digest buffer</td>
	</tr>		</tr>
	</table>		</table>
		+	</div>
	<!--Restriction Digest-->		<!--Restriction Digest-->
Line 3,216:		Line 3,733:
	<!--AGE-->		<!--AGE-->
	<strong>AGE</strong>		<strong>AGE</strong>
		+	<div class="m_table">
	<table class="materials">		<table class="materials">
	<tr>		<tr>
Line 3,229:		Line 3,747:
	<tr>		<tr>
	<td>1</td>		<td>1</td>
-	<td>1kb Ladder</td>	+	<td>p427-TEF(ア)</td>
-	<td></td>	+	<td>CuMTS3</td>
-	<td></td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td></td>	+	<td>Non Treated</td>
	</tr>		</tr>
	<tr>		<tr>
Line 3,238:		Line 3,756:
	<td>p427-TEF(ア)</td>		<td>p427-TEF(ア)</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>N.D</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>3</td>		<td>3</td>
-	<td>p427-TEF(ア)</td>	+	<td>p427-TEF(イ)</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td>Non Treated</td>
	</tr>		</tr>
	<tr>		<tr>
Line 3,252:		Line 3,770:
	<td>p427-TEF(イ)</td>		<td>p427-TEF(イ)</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>N.D</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>5</td>		<td>5</td>
-	<td>p427-TEF(イ)</td>	+	<td>p427-TEF(エ)</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td>Non Treated</td>
	</tr>		</tr>
	<tr>		<tr>
Line 3,266:		Line 3,784:
	<td>p427-TEF(エ)</td>		<td>p427-TEF(エ)</td>
	<td>CuMTS3</td>		<td>CuMTS3</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>N.D</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>7</td>		<td>7</td>
-	<td>p427-TEF(エ)</td>	+	<td>p427-TEF(d)</td>
-	<td>CuMTS3</td>	+	<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td>Non Treated</td>
	</tr>		</tr>
	<tr>		<tr>
Line 3,280:		Line 3,798:
	<td>p427-TEF(d)</td>		<td>p427-TEF(d)</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>N.D</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>9</td>		<td>9</td>
-	<td>p427-TEF(d)</td>	+	<td>p427-TEF(h)</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td>Non Treated</td>
	</tr>		</tr>
	<tr>		<tr>
Line 3,294:		Line 3,812:
	<td>p427-TEF(h)</td>		<td>p427-TEF(h)</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>N.D</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
	</tr>		</tr>
	<tr>		<tr>
	<td>11</td>		<td>11</td>
-	<td>p427-TEF(h)</td>	+	<td>p427-TEF(l)</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>Spe1, Xho1</td>	+	<td>Non Treated</td>
	</tr>		</tr>
	<tr>		<tr>
Line 3,308:		Line 3,826:
	<td>p427-TEF(l)</td>		<td>p427-TEF(l)</td>
	<td>CuMTS62</td>		<td>CuMTS62</td>
-	<td>P-F1, P-R</td>	+	<td>pGEX6P-2F1, pGEX6P-2R</td>
-	<td>N.D</td>	+	<td><em>Spe</em>1, <em>Xho</em>1</td>
-	</tr>	+
-	<tr>	+
-	<td>13</td>	+
-	<td>p427-TEF(l)</td>	+
-	<td>CuMTS62</td>	+
-	<td>P-F1, P-R</td>	+
-	<td>Spe1, Xho1</td>	+
	</tr>		</tr>
	</table>		</table>
		+	</div>
	Marker: 1kb Ladder		Marker: 1kb Ladder
	<br>		<br>
		+	<img src="/wiki/images/5/59/Kit_July9_c.jpg">
	<!--AGE-->		<!--AGE-->
	<br>		<br>
-	</p>	+	</p>
-	<h2>August</h2>	+	</div>
		+	</div>
		+
		+	<div class="accordion">
		+	<h2><a href="javascript:void(0);">August</a></h2>
	<div class="scroll"></div>		<div class="scroll"></div>
		+	<div class="ac">
		+	<h3>August 19, 2014</h3>
	<p class="sentence">		<p class="sentence">
		+	KOBAYASHI
	<br>		<br>
		+	Conduct mutagenesis to remove an illegal restriction site (<em>Eco</em>R1)
	<br>		<br>
-	<br>a	+	<strong>InversePCR</strong>
-	<br>a	+	<div class="m_table">
-	<br>a	+	<table class="materials">
-	<br>a	+	<tr>
-	<br>a	+	<th colspan="4">Sample</th>
-	<br><br>	+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	<th>DNA polymerase</th>
		+	</tr>
		+	<tr>
		+	<td>pGEX6P-2</td>
		+	<td>CuMTS3</td>
		+	<td>Terpinene3-mutagenesis-FP, Terpinene3-mutagenesis-RP</td>
		+	<td>KOD-Plus</td>
		+	</tr>
		+	</table>
		+	</div>
		+	(TOYOBO, KOD-Plus-Mutagenesis Kit)
		+	Start from KOD -Plus- Mutagenesis Kit protocol No.2.
	<br>		<br>
-	<br>a
-	<br>a
-	<br>a
-	<br>a
-	<br>a
	<br>		<br>
-	<br><br>	+	</p>
		+
		+
		+	<h3>August 20, 2014</h3>
		+	<p class="sentence">
		+	KOBAYASHI
	<br>		<br>
-	<br>a	+	Restart mutagenesis from KOD -Plus- Mutagenesis Kit protocol No.3 to obtain plasmid (muta-CuMTS3 in pGEX-6P-2)
-	<br>a	+
-	<br>a	+
-	<br>a	+
-	<br>a	+
	<br>		<br>
-	<br><br>	+	<strong>Transformation</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="4">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td rowspan="2">DH5α</td>
		+	<td rowspan="2">pGEX6P-2</td>
		+	<td rowspan="2">muta-CuMTS3</td>
		+	<td>Terpinene3-mutagenesis-FP and Terpinene3-mutagenesis-RP</td>
		+	</tr>
		+	</table>
		+	</div>
	<br>		<br>
-	<br>a
-	<br>a
-	<br>a
-	<br>a
-	<br>a
	<br>		<br>
-	<br><br>	+	</p>
		+
		+	<h3>August 21, 2014</h3>
		+	<p class="sentence">
		+	KOBAYASHI
	<br>		<br>
-	<br>a	+	<strong>Colony Isolation</strong><br>
-	<br>a	+	Isolate each colony from two plates which were inoculated overnight
-	<br>a	+	<div class="m_table">
-	<br>a	+	<table class="materials">
-	<br>a	+	<tr>
		+	<th colspan="4">Sample</th>
		+	<th rowspan="2">Section</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td>Terpinene3-mutagenesis-FP,<br>Terpinene3-mutagenesis-RP</td>
		+	<td>あ-み</td>
		+	</tr>
		+	</table>
		+	</div>
		+	Select 5 isolated colonies whose code names are あ,か,さ,た and な and miniprep them.
	<br>		<br>
	<br>		<br>
		+	</p>
		+
		+	<h3>August 22, 2014</h3>
		+	<p class="sentence">
		+	KOBAYASHI
	<br>		<br>
-	<br></p>	+	<strong>Restriction Digest</strong>
-	<h2>September</h2>	+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="3">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	<th rowspan="2">Buffer</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	</tr>
		+	<tr>
		+	<td>DH5α  あ</td>
		+	<td>pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Sal</em>1</td>
		+	<td>Fast Digest buffer</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α  か</td>
		+	<td>pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Sal</em>1</td>
		+	<td>Fast Digest buffer</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α  さ</td>
		+	<td>pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Sal</em>1</td>
		+	<td>Fast Digest buffer</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α  た</td>
		+	<td>pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Sal</em>1</td>
		+	<td>Fast Digest buffer</td>
		+	</tr>
		+	<tr>
		+	</table>
		+	</div>
		+	<br>
		+	<strong>AGE</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Lane</th>
		+	<th colspan="4">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	</tr>
		+	<tr>
		+	<th>Marker</th>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	</tr>
		+	<tr>
		+	<td>1</td>
		+	<td>1kb Ladder</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>2</td>
		+	<td></td>
		+	<td>DH5α  あ</td>
		+	<td>pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Sal</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>3</td>
		+	<td></td>
		+	<td>DH5α  か</td>
		+	<td>pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Sal</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>4</td>
		+	<td></td>
		+	<td>DH5α  さ</td>
		+	<td>pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Sal</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>5</td>
		+	<td></td>
		+	<td>DH5α  た</td>
		+	<td>pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Sal</em>1</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<strong>Restriction Digest</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="3">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	<th rowspan="2">Buffer</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	</tr>
		+	<tr>
		+	<td>DH5α  か</td>
		+	<td>pGEX6P-2</td>
		+	<td>CuMTS3</td>
		+	<td><em>Eco</em>R1</td>
		+	<td>Fast Digest buffer</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α  か</td>
		+	<td>pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Eco</em>R1</td>
		+	<td>Fast Digest buffer</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<strong>AGE</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Lane</th>
		+	<th colspan="4">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	</tr>
		+	<tr>
		+	<th>Marker</th>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	</tr>
		+	<tr>
		+	<td>1</td>
		+	<td>1kb Ladder</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>2</td>
		+	<td></td>
		+	<td>DH5α  か</td>
		+	<td>pGEX6P-2</td>
		+	<td>CuMTS3</td>
		+	<td><em>Eco</em>R1</td>
		+	</tr>
		+	<tr>
		+	<td>3</td>
		+	<td></td>
		+	<td>DH5α  か</td>
		+	<td>pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Eco</em>R1</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<img src="/wiki/images/b/bc/Kit_August22_c.jpg">
		+	An illegal <em>Eco</em>R1 restriction site was removed.<br>
		+	<br>
		+	<strong>PCR</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="3">Sample</th>
		+	<th rowspan="2">DNA polymerase</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>か  pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td>terpinene3-prefix and terpinene3-suffix</td>
		+	<td>KOD-FX-NEO-Plus</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<strong>AGE</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Lane</th>
		+	<th colspan="4">Sample</th>
		+	<th rowspan="2">DNA polymerase</th>
		+	</tr>
		+	<tr>
		+	<th>Marker</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>1</td>
		+	<td>1kb Ladder</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>2</td>
		+	<td></td>
		+	<td>か  pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td>terpinene3-prefix and terpinene3-suffix</td>
		+	<td>KOD-FX-NEO-Plus</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<img src="/wiki/images/7/72/Kit_August22_1_c.jpg">
		+	<br>
		+	<br>
		+	</p>
		+
		+	<h3>August 30, 2014</h3>
		+	KOBAYASHI
		+	<br>
		+	<strong>PCR</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="3">Sample</th>
		+	<th rowspan="2">DNA polymerase</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>か  pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td>terpinene3-prefix-<em>Xba</em>1 and terpinene3-suffix-<em>Spe</em>1</td>
		+	<td><em>Taq</em></td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<strong>AGE</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Lane</th>
		+	<th colspan="4">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Marker</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>1</td>
		+	<td>1kb Ladder</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>2</td>
		+	<td></td>
		+	<td>か  pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td>terpinene3-prefix-<em>Xba</em>1 and terpinene3-suffix-<em>Spe</em>1</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<img src="/wiki/images/3/31/Kit_August30_c.jpg">
		+	Made total amount of 295 μL of PCR samples.
		+	<br>
		+	</tr>
		+	<br>
		+	</p>
		+	</div>
		+	</div>
		+
		+	<div class="accordion">
		+	<h2><a href="javascript:void(0);">September</a></h2>
	<div class="scroll"></div>		<div class="scroll"></div>
		+	<div class="ac">
	<p class="sentence">		<p class="sentence">
		+	<h3>September 18, 2014</h3>
		+	KOBAYASHI
	<br>		<br>
		+	<strong>Miniprep</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="3">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	</tr>
		+	</table>
		+	</div>
	<br>		<br>
-	<br>a	+	<strong>Restriction Digest</strong>
-	<br>a	+	<div class="m_table">
-	<br>a	+	<table class="materials">
-	<br>a	+	<tr>
-	<br>a	+	<th colspan="3">Sample</th>
-	<br><br>	+	<th rowspan="2">Buffer</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Enzyme</th>
		+	</tr>
		+	<tr>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td><em>Xba</em>1, <em>Spe</em>1</td>
		+	<td>Fast Digest buffer</td>
		+	</tr>
		+	<tr>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	</table>
		+	</div>
		+	</tr>
	<br>		<br>
-	<br>a	+	<strong>AGE</strong>
-	<br>a	+	<div class="m_table">
-	<br>a	+	<table class="materials">
-	<br>a	+	<tr>
-	<br>a	+	<th rowspan="2">Lane</th>
		+	<th colspan="4">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	</tr>
		+	<tr>
		+	<th>Marker</th>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	</tr>
		+	<tr>
		+	<td>1</td>
		+	<td>1kb Ladder</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>2</td>
		+	<td></td>
		+	<td>DH5α</td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>3</td>
		+	<td></td>
		+	<td>DH5α</td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td><em>Xba</em>1, <em>Spe</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>4</td>
		+	<td></td>
		+	<td>DH5α</td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	</tr>
		+	</table>
		+	</div>
		+	<img src="/wiki/images/2/29/Kit_September18_c.jpg">
	<br>		<br>
-	<br><br>	+	<strong>PCR</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="3">Sample</th>
		+	<th rowspan="2">DNA polymerase</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>か pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td>terpinene3-prefix-<em>Xba</em>1 and terpinene3-suffix-<em>Spe</em>1</td>
		+	<td><em>Taq</em></td>
		+	</tr>
		+	</table>
		+	</div>
	<br>		<br>
-	<br>a	+	<strong>DNA Refinement 1</strong>
-	<br>a	+	<div class="m_table">
-	<br>a	+	<table class="materials">
-	<br>a	+	<tr>
-	<br>a	+	<th colspan="2">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>muta-CuMTS3</td>
		+	<td>terpinene3-prefix-<em>Xba</em>1 and terpinene3-suffix-<em>Spe</em>1</td>
		+	</tr>
		+	</table>
		+	</div>
		+	</tr>
	<br>		<br>
-	<br><br>	+	<strong>Restriction Digest</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="3">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	<th rowspan="2">Buffer</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Xba</em>1, <em>Spe</em>1</td>
		+	<td>Fast Digest</td>
		+	</tr>
		+	<tr>
		+	<td>か pGEX6P-2</td>
		+	<td>muta-CuMTS3</td>
		+	<td>terpinene3-prefix-<em>Xba</em>1 and terpinene3-suffix-<em>Spe</em>1</td>
		+	<td><em>Xba</em>1, <em>Spe</em>1</td>
		+	<td>Fast Digest</td>
		+	</tr>
		+	<tr>
		+	</table>
		+	</div>
		+	</tr>
	<br>		<br>
-	<br>a	+	<strong>DNA Refinement 2</strong>
-	<br>a	+	<div class="m_table">
-	<br>a	+	<table class="materials">
-	<br>a	+	<tr>
-	<br>a	+	<th colspan="2">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>muta-CuMTS3</td>
		+	<td>terpinene3-prefix-<em>Xba</em>1 and terpinene3-suffix-<em>Spe</em>1</td>
		+	</tr>
		+	<tr>
		+	</table>
		+	</div>
		+	</tr>
	<br>		<br>
-	<br><br>	+	<strong>Ligation</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="3">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>DNA</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td>terpinene3-prefix-<em>Xba</em>1 and terpinene3-suffix-<em>Spe<em>1</td>
		+	<td><em>Spe</em>1, <em>Xba</em>1</td>
		+	</tr>
		+	<tr>
		+	</table>
		+	</div>
		+	</tr>
		+	DNA ligase: DNA Ligation Kit ‹Mighty Mix› (TAKARA BIO INC.)
	<br>		<br>
-	<br>a
-	<br>a
-	<br>a
-	<br>a
-	<br>a
	<br>		<br>
		+	<strong>Transformation</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="4">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td>terpinene3-prefix-<em>Xba</em>1 and terpinene3-suffix-<em>Spe</em>1</td>
		+	</tr>
		+	<tr>
		+	</table>
		+	</div>
		+	</tr>
	<br>		<br>
		+	</p>
		+
		+
		+	<h3>September 19, 2014</h3>
		+	KOBAYASHI
		+	<br>
		+	<strong>Colony Isolation</strong>
		+	<br>
		+	Isolate each colony from the plates which were inoculated overnight.
		+	<br>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="4">Sample</th>
		+	<th rowspan="2">Section</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td>terpinene3-prefix-<em>Xba</em>1 and terpinene3-suffix-<em>Spe</em>1</td>
		+	<td>A-P</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td>terpinene3-prefix-<em>Xba</em>1 and terpinene3-suffix-<em>Spe</em>1</td>
		+	<td>ア-タ</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<br>
		+	<strong>Colony Sweep</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Lane</th>
		+	<th colspan="3">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	</tr>
		+	<tr>
		+	<td>1</td>
		+	<td>DH5α</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	</tr>
		+	<tr>
		+	<td>2</td>
		+	<td>ア</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>3</td>
		+	<td>イ</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>4</td>
		+	<td>ウ</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>5</td>
		+	<td>エ</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>6</td>
		+	<td>オ</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>7</td>
		+	<td>カ</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>8</td>
		+	<td>キ</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>9</td>
		+	<td>ク</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>10</td>
		+	<td>ケ</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>11</td>
		+	<td>コ</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>12</td>
		+	<td>サ</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>13</td>
		+	<td>シ</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>14</td>
		+	<td>ス</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>15</td>
		+	<td>セ</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>16</td>
		+	<td>ソ</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>17</td>
		+	<td>タ</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	</table>
		+	</div>
		+	<img src="/wiki/images/4/40/Kit_September19_c.jpg">
		+	<br>
		+	Targeted colonies were identified in セ and ソ.
		+	<br>
		+	<br>
		+	<strong>Colony Sweep</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Lane</th>
		+	<th colspan="3">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	</tr>
		+	<tr>
		+	<td>1</td>
		+	<td>DH5α</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	</tr>
		+	<tr>
		+	<td>2</td>
		+	<td>A</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>3</td>
		+	<td>B</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>4</td>
		+	<td>C</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>5</td>
		+	<td>D</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>6</td>
		+	<td>E</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>7</td>
		+	<td>F</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>8</td>
		+	<td>G</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>9</td>
		+	<td>H</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>10</td>
		+	<td>I</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>11</td>
		+	<td>J</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>12</td>
		+	<td>K</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>13</td>
		+	<td>L</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>14</td>
		+	<td>M</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>15</td>
		+	<td>N</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>16</td>
		+	<td>O</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>17</td>
		+	<td>P</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	</tr>
		+
		+	<img src="/wiki/images/e/e6/Kit_September19_1_c.jpg">
		+	<br>
		+	Targeted colonies were identified in colony N and F.
		+	<br>
		+	<br>
		+	Select セ, ソ, N, F
		+	<br>
		+	<br>
		+	<strong>Main Culture</strong>
		+	<br>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="4">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>DH5α(N)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td>----------</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α(F)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td>----------</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α(セ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td>----------</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α(ソ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td>----------</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	Cultivate these samples in 3mL of LB medium with ChlPhe at 30°C overnight.
		+	<br>
		+	</p>
		+
		+
		+	<h3>September 20, 2014</h3>
		+	KOBAYASHI
		+	<br>
		+	<strong>Miniprep</strong>
		+	<br>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="3">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>DH5α(N)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α(F)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α(セ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α(ソ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	Enzyme: E/P and <em>Xba</em>1
		+	<br>
		+	<br>
		+	<strong>Restriction Digest</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="4">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	<th rowspan="2">Buffer</th>
		+	</tr>
		+	<tr>
		+	<th>Marker</th>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>DH5α (セ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>DH5α (ソ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>DH5α (F)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>DH5α (N)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>DH5α (セ)</td>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>DH5α (ソ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>DH5α (F)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>DH5α (N)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td><em>Xba</em>1</td>
		+	<td>FD buffer</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>DH5α (セ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Xba</em>1</td>
		+	<td>FD buffer</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>DH5α (ソ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Xba</em>1</td>
		+	<td>FD buffer</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>DH5α (F)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Xba</em>1</td>
		+	<td>FD buffer</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>DH5α (N)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Xba</em>1</td>
		+	<td>FD buffer</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<strong>AGE</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Lane</th>
		+	<th colspan="4">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	</tr>
		+	<tr>
		+	<th>Marker</th>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	</tr>
		+	<tr>
		+	<td>1</td>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>2</td>
		+	<td></td>
		+	<td>DH5α (セ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>3</td>
		+	<td></td>
		+	<td>DH5α (ソ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>4</td>
		+	<td></td>
		+	<td>DH5α (F)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>5</td>
		+	<td></td>
		+	<td>DH5α (N)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>6</td>
		+	<td>1kb Ladder</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>7</td>
		+	<td></td>
		+	<td>DH5α (セ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>8</td>
		+	<td></td>
		+	<td>DH5α (ソ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>9</td>
		+	<td></td>
		+	<td>DH5α (F)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>10</td>
		+	<td></td>
		+	<td>DH5α (N)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>11</td>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>12</td>
		+	<td>1kb Ladder</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>13</td>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td><em>Xba</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>14</td>
		+	<td></td>
		+	<td>DH5α (セ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Xba</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>15</td>
		+	<td></td>
		+	<td>DH5α (ソ)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Xba</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>16</td>
		+	<td></td>
		+	<td>DH5α (F)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Xba</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>17</td>
		+	<td></td>
		+	<td>DH5α (N)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td><em>Xba</em>1</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<img src="/wiki/images/e/e5/Kit_September20_c.jpg">
		+	<div class="clear"><hr /></div>
		+	Sample F was selected as a submission plasmid.
		+	<br>
		+	<br>
		+	<strong>Main Culture</strong>
		+	<br>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="4">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>DH5α(F)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td>----------</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α(F)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td>----------</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α(F)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td>----------</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	Cultivate these samples in 3mL of LB medium with ChlPhe at 30°C overnight.
		+	<br>
		+	</p>
		+
		+
		+	<h3>September 21, 2014</h3>
		+	KOBAYASHI
		+	<br>
		+	<strong>Miniprep</strong>
		+	<br>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="4">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>DH5α(F)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>DH5α(F)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>DH5α(F)</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<br>
		+	<strong>Restriction Digest</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="5">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	<th rowspan="2">Buffer</th>
		+	</tr>
		+	<tr>
		+	<th>Marker</th>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>1kb Ladder</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td><em>Eco</em>R1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td><em>Pst</em>1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td><em>Spe</em>1</td>
		+	<td>FD buffer</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td><em>Xba</em>1</td>
		+	<td>FD buffer</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td><em>Spe</em>1, <em>Xba</em>1</td>
		+	<td>FD buffer</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>λDNA-HindⅢ</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>F</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td><em>Eco</em>R1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>F</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td><em>Pst</em>1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>F</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>F</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td><em>Spe</em>1</td>
		+	<td>FD buffer</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>F</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td><em>Xba</em>1</td>
		+	<td>FD buffer</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>F</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td><em>Spe</em>1, <em>Xba</em>1</td>
		+	<td>FD buffer</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>F</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<strong>AGE</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Lane</th>
		+	<th colspan="5">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	</tr>
		+	<tr>
		+	<th>Marker</th>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>1</td>
		+	<td>1kb Ladder</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>2</td>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td><em>Eco</em>R1</td>
		+	</tr>
		+	<tr>
		+	<td>3</td>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td><em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>4</td>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>5</td>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td><em>Spe</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>6</td>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td><em>Xba</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>7</td>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td><em>Spe</em>1, <em>Xba</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>8</td>
		+	<td></td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>BBa_J04450</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>9</td>
		+	<td>λDNA-HindⅢ</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>10</td>
		+	<td></td>
		+	<td>F</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td><em>Eco</em>R1</td>
		+	</tr>
		+	<tr>
		+	<td>11</td>
		+	<td></td>
		+	<td>F</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td><em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>12</td>
		+	<td></td>
		+	<td>F</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>13</td>
		+	<td></td>
		+	<td>F</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td><em>Spe</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>14</td>
		+	<td></td>
		+	<td>F</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td><em>Xba</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>15</td>
		+	<td></td>
		+	<td>F</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td><em>Spe</em>1, <em>Xba</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>16</td>
		+	<td></td>
		+	<td>F</td>
		+	<td>pSB1C3</td>
		+	<td>muta-CuMTS3</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	</table>
		+	</div>
		+	<img src="/wiki/images/d/d7/Kit_September21_c.jpg">
		+
	<br>		<br>
	<br></p>		<br></p>
-	<h2>October</h2>	+	</div>
		+	</div>
		+
		+	<div class="accordion">
		+	<h2><a href="javascript:void(0);">October</a></h2>
	<div class="scroll"></div>		<div class="scroll"></div>
		+	<div class="ac">
	<p class="sentence">		<p class="sentence">
		+
		+	<h3>October 1, 2014</h3>
		+	KOBAYASHI
	<br>		<br>
		+	<strong>Protein Extraction (<em>S. cerevisiae</em>)</strong>
	<br>		<br>
-	<br>a	+	<div class="m_table">
-	<br>a	+	<table class="materials">
-	<br>a	+	<tr>
-	<br>a	+	<th colspan="4">Sample</th>
-	<br>a	+	</tr>
-	<br><br>	+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE2</td>
		+	<td>pGEX6P-2F1, pGEX6P-2R</td>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTS3</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTS62</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	</table>
		+	</div>
	<br>		<br>
-	<br>a
-	<br>a
-	<br>a
-	<br>a
-	<br>a
	<br>		<br>
-	<br><br>	+	<strong>SDS-PAGE</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Lane</th>
		+	<th colspan="4">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>1</td>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>2</td>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE2</td>
		+	<td>pGEX6P-2F1, pGEX6P-2R</td>
		+	</tr>
		+	<tr>
		+	<td>3</td>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTS3</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	<tr>
		+	<td>4</td>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTS62</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	</table>
		+	</div>
	<br>		<br>
-	<br>a	+	</tr>
-	<br>a	+	</p>
-	<br>a	+
-	<br>a	+
-	<br>a	+	<h3>October 2, 2014</h3>
		+	KOBAYASHI
	<br>		<br>
-	<br><br>	+	<strong>Preculture</strong>
	<br>		<br>
-	<br>a	+	<div class="m_table">
-	<br>a	+	<table class="materials">
-	<br>a	+	<tr>
-	<br>a	+	<th colspan="4">Sample</th>
-	<br>a	+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTS3</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	</table>
		+	</div>
	<br>		<br>
-	<br><br>	+	Cultivate these samples in 3mL of SD medium with G418 at 30°C overnight.
	<br>		<br>
-	<br>a
-	<br>a
-	<br>a
-	<br>a
-	<br>a
	<br>		<br>
		+	<strong>Western blot</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Lane</th>
		+	<th colspan="4">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>1</td>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>2</td>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE2</td>
		+	<td>pGEX6P-2F1, pGEX6P-2R</td>
		+	</tr>
		+	<tr>
		+	<td>3</td>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTS3</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	<tr>
		+	<td>4</td>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTS62</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	</table>
		+	</div>
	<br>		<br>
		+	<img src="/wiki/images/e/e0/Kit_October2_c.jpg">
		+	</p>
		+
		+	<h3>October 3, 2014</h3>
		+	KOBAYASHI
	<br>		<br>
-	<br></p>	+	<strong>Main culture</strong>
		+	<br>
		+	Cultivate following samples in Big Scale with G418 at 28°C overnight in shaken culture.
		+	<br>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="4">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Host</th>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTS3</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<br>
		+	<strong>Restriction Digest</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="3">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	<th rowspan="2">Buffer</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Spe</em>1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Xba</em>1, <em>Pst</em>1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	<tr>
		+	<td>Linearized pSB1A3</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	<td>Buffer H</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<br>
		+	<strong>AGE</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Lane</th>
		+	<th rowspan="2">Marker</th>
		+	<th colspan="3">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>1</td>
		+	<td>1kb Ladder</td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>2</td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Spe</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>3</td>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Xba</em>1, <em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>4</td>
		+	<td></td>
		+	<td>Linearized pSB1A3</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<br>
		+	<strong>DNA Refinement 1</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th colspan="3">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Spe</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Xba</em>1, <em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Xba</em>1, <em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>Linearized pSB1A3</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1, <em>Dpn</em>1</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<br>
		+	<strong>Ligation</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Vector</th>
		+	<th colspan="2">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	</tr>
		+	<tr>
		+	<th>Insert1</th>
		+	<th>Insert2</th>
		+	</tr>
		+	<tr>
		+	<td>pSB1A3</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Xba</em>1, <em>Spe</em>1, <em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>pSB1A3</td>
		+	<td></td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Xba</em>1, <em>Spe</em>1, <em>Pst</em>1</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	DNA ligase: DNA Ligation Kit ‹Mighty Mix› (TAKARA BIO INC.)
		+	<br>
		+	<br>
		+	<strong>Transformation</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Host</th>
		+	<th colspan="3">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert1</th>
		+	<th>Insert2</th>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1A3</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1A3</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	</tr>
		+	</p>
		+
		+
		+	<h3>October 4, 2014</h3>
		+	KOBAYASHI
		+	<br>
		+	<strong>Colony Isolation</strong>
		+	<br>
		+	Isolate each colony from the plates which were inoculated overnight.
		+	<br>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Host</th>
		+	<th colspan="3">Sample</th>
		+	<th rowspan="2">Section</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1A3</td>
		+	<td></td>
		+	<td></td>
		+	<td>1-16</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1A3</td>
		+	<td></td>
		+	<td></td>
		+	<td>a-p</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<br>
		+	<strong>Monoterpene Extraction</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Host</th>
		+	<th colspan="3">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTS3</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	Samples were stocked in -4℃.
		+	<br>
		+	<br>
		+	<strong>Preculture</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<th rowspan="2">Host</th>
		+	<th colspan="3">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTSE2</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTS62</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	Cultivate samples in 3mL of SD medium with G418 at 30°C overnight.
		+	<br>
		+	</tr>
		+	</p>
		+
		+
		+	<h3>October 5, 2014</h3>
		+	KOBAYASHI
		+	<br>
		+	<strong>Miniprep</strong>
		+	<br>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Host</th>
		+	<th colspan="2">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1A3</td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1A3</td>
		+	<td></td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<br>
		+	<strong>Restriction Digest and AGE</strong>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Host</th>
		+	<th colspan="2">Sample</th>
		+	<th rowspan="2">Enzyme</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td>DH5α</td>
		+	<td>pSB1C3</td>
		+	<td></td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	</tr>
		+	<tr>
		+	<td></td>
		+	<td>pSB1C3</td>
		+	<td>K1448000</td>
		+	<td><em>Eco</em>R1, <em>Pst</em>1</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	</tr>
		+	</p>
		+
		+	<h3>October 8, 2014</h3>
		+	KOBAYASHI
		+	<br>
		+	<strong>GC-MS analysis</strong>
		+	<br>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Host</th>
		+	<th colspan="3">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTS3</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<strong>Preculture</strong>
		+	<br>
		+	<div class="m_table">
		+
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Host</th>
		+	<th colspan="3">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTS3</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	</tr>
		+	</p>
		+
		+
		+	<h3>October 9, 2014</h3>
		+	KOBAYASHI
		+	<br>
		+	<strong>Main Culture</strong>
		+	<br>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Host</th>
		+	<th colspan="3">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTS3</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	</tr>
		+	</p>
		+
		+
		+	<h3>October 10, 2014</h3>
		+	KOBAYASHI
		+	<br>
		+	<strong>GC Analysis</strong>
		+	<br>
		+	<div class="m_table">
		+	<table class="materials">
		+	<tr>
		+	<th rowspan="2">Host</th>
		+	<th colspan="3">Sample</th>
		+	</tr>
		+	<tr>
		+	<th>Vector</th>
		+	<th>Insert</th>
		+	<th>Primer</th>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td></td>
		+	<td></td>
		+	</tr>
		+	<tr>
		+	<td>BY4247</td>
		+	<td>p427-TEF</td>
		+	<td>CuMTS3</td>
		+	<td>pGEX6P-2F2, pGEX6P-2R</td>
		+	</tr>
		+	</table>
		+	</div>
		+	<br>
		+	<img src="/wiki/images/b/b1/Kit_October10_c.jpg">
		+	<div class="clear"><hr /></div>
		+	For More Information: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1448000">BBa_K1448000</a>
		+	</p>
		+	</div>
		+	</div>
		+	</div>
	<div class="clear"><hr /></div>		<div class="clear"><hr /></div>
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		+	margin:10px 0px;
		+	}
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Latest revision as of 01:13, 18 October 2014