Team:Tongji/lab-archives

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                <a  href="https://2014.igem.org/Team:Tongji">iGEM Tongji 2014</a>
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                <a  href="https://2014.igem.org/Team:Tongji/lab-archives">Lab Archives</a>
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    <h1>Lab Archives</h1>
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            <h1>Protocol</h1>
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          <p class="lead">Here you can find our essential protocols. Please click "Download" buttons.</p>
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/81/Tongji-Notebook.png" alt="notebook">
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            <div class="caption">
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              <h5>Protocol of PCR</h5>
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              <p>PCR of XynB, ArfB and ManA1.</p>
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              <p><a  onclick=window.parent.location.href="https://static.igem.org/mediawiki/2014/d/d2/IGEM_Tongji_Protocol_PCR.pdf" class="btn btn-primary">Download</a></p>
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/81/Tongji-Notebook.png" alt="notebook">
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            <div class="caption">
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              <h5>Protocol of Bacterium Cultivation</h5>
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              <p>Cultivation of <em>Clostridium stercorarium</em>.</p>
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              <div class="nelson">
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              <p><a  href="https://static.igem.org/mediawiki/2014/9/93/IGEM_Tongji_Protocol_CSCultivation.pdf" class="btn btn-primary">Download</a></p>
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            <h1>Notebook</h1>
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          </div>
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          <p class="lead">Here you can find our essential notebooks. Please click "Download" buttons.</p>
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        <div class="row ">
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/81/Tongji-Notebook.png" alt="notebook">
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            <div class="caption">
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              <h5>Vector Construction</h5>
 +
              <p>Report for expression vector construction</p>
 +
              <div class="nelson">
 +
              <p><a  href="https://static.igem.org/mediawiki/2014/5/51/Report_for_expression_vector_construction2.pdf" class="btn btn-primary">Download</a></p>
 +
              </div>
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        <br>
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/81/Tongji-Notebook.png" alt="notebook">
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            <div class="caption">
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              <h5>Expression Validation</h5>
 +
              <p>Report for protein expression in <em>E.coli</em></p>
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              <div class="nelson">
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              <p><a href="https://static.igem.org/mediawiki/2014/0/07/Report_for_protein_expression_in_Ecoli2.pdf" class="btn btn-primary">Download</a></p>
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            <div class="caption">
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              <h5>Function Validation</h5>
 +
              <p>Report for DNS reaction by xynB</p>
 +
              <div class="nelson">
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              <p><a href="https://static.igem.org/mediawiki/2014/7/7a/Report_for_enzyme_activity.pdf" class="btn btn-primary">Download</a></p>
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              </div>
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            <div class="caption">
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              <h5>Hot-resistance Survey</h5>
 +
              <p>Report for DNS reaction by xynB in high temperature.</p>
 +
              <div class="nelson">
 +
              <p><a href="https://static.igem.org/mediawiki/2014/7/7a/Report_for_enzyme_activity.pdf" class="btn btn-primary">Download</a></p>
 +
              </div>
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/81/Tongji-Notebook.png" alt="notebook">
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            <div class="caption">
 +
              <h5>Bleaching Modeling</h5>
 +
              <p>Report for measuring xynB's bleaching effect</p>
 +
              <div class="nelson">
 +
              <p><a href="https://static.igem.org/mediawiki/2014/5/5e/Report_for_measuring_xynBs_bleaching_effect.pdf" class="btn btn-primary">Download</a></p>
 +
              </div>
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        <!-- Contents
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        <section id="contents">
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          <div class="page-header">
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            <h1>Parts</h1>
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          </div>
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          <p class="lead">We prepared two versions of our parts for you. One is iGEM standard parts, with the official vector. They are universal but may not be so efficient. The other one is our modified parts, with the pET vector and can perfectly work with BL21(DE3) <em>E.coli</em> in our wetlab experiment. <strong>You can order from iGEM Registry for our iGEM standard parts or directly <a id="Jin" href="#" rel="tooltip" data-placement="top" data-original-title="Delivery fee is required. Please contact us via this email: jiangliang2012@hotmail.com"><strong>order</strong></a> from us for modified parts.</strong>  </p>
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          <div class="thumbnail span3"> <img src="https://static.igem.org/mediawiki/2014/8/88/Parts-Tongji.png" alt="notebook">
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            <div class="caption">
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              <h5>pET22b(+)_x1 Map</h5>
 +
              <p>a pET22b(+) vector harboring XynB (without C-terminal His-tag)</p>
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              <div class="nelson">
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                <!-- Button to trigger modal -->
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            <div class="caption">
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              <h5>pET22b(+)_x2 Map</h5>
 +
              <p>a pET22b(+) vector harboring XynB (with C-terminal His-tag)</p>
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              <div class="nelson">
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            <div class="caption">
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              <h5>pET22b(+)_ArfB Map</h5>
 +
              <p>a pET22b(+) vector harboring ArfB (without C-terminal His-tag)</p>
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              <div class="nelson">
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            <div class="caption">
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              <h5>pET22b(+)_ManA1 Map</h5>
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              <p>a pET22b(+) vector harboring ManA1 (without C-terminal His-tag)</p>
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              <h3 id="myModalLabel">pET22b(+)_x1 Map</h3>
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              <img src="https://static.igem.org/mediawiki/2014/3/3d/TongjiXynB1.png">
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            <div class="caption">
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              <h5>pSB1C3_XynB Map</h5>
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              <p>a pSB1C3 vector harboring XynB </p>
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            <div class="caption">
 +
              <h5>pSB1C3_ArfB Map</h5>
 +
              <p>a pSB1C3 vector harboring XynB </p>
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              <div class="nelson">
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                <!-- Button to trigger modal -->
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              <p><a href="#myModal6" role="button" class="btn btn-primary" data-toggle="modal">Preview</a></p>
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            <div class="caption">
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              <h5>pSB1C3_ManA1 Map</h5>
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              <p>a pSB1C3 vector harboring XynB </p>
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Latest revision as of 03:43, 18 October 2014

iGEM Tongji 2014

Lab Archives

Protocol

Here you can find our essential protocols. Please click "Download" buttons.

notebook
Protocol of PCR

PCR of XynB, ArfB and ManA1.

Download


notebook
Protocol of Bacterium Cultivation

Cultivation of Clostridium stercorarium.

Download


Notebook

Here you can find our essential notebooks. Please click "Download" buttons.

notebook
Vector Construction

Report for expression vector construction

Download


notebook
Expression Validation

Report for protein expression in E.coli

Download


notebook
Function Validation

Report for DNS reaction by xynB

Download


notebook
Hot-resistance Survey

Report for DNS reaction by xynB in high temperature.

Download


notebook
Bleaching Modeling

Report for measuring xynB's bleaching effect

Download


Parts

We prepared two versions of our parts for you. One is iGEM standard parts, with the official vector. They are universal but may not be so efficient. The other one is our modified parts, with the pET vector and can perfectly work with BL21(DE3) E.coli in our wetlab experiment. You can order from iGEM Registry for our iGEM standard parts or directly order from us for modified parts.

notebook
pET22b(+)_x1 Map

a pET22b(+) vector harboring XynB (without C-terminal His-tag)

Preview


notebook
pET22b(+)_x2 Map

a pET22b(+) vector harboring XynB (with C-terminal His-tag)

Preview


notebook
pET22b(+)_ArfB Map

a pET22b(+) vector harboring ArfB (without C-terminal His-tag)

Preview


notebook
pET22b(+)_ManA1 Map

a pET22b(+) vector harboring ManA1 (without C-terminal His-tag)

Preview


notebook
pSB1C3_XynB Map

a pSB1C3 vector harboring XynB

Preview


notebook
pSB1C3_ArfB Map

a pSB1C3 vector harboring XynB

Preview


notebook
pSB1C3_ManA1 Map

a pSB1C3 vector harboring XynB

Preview


Tongji University, 2014

Retrieved from "http://2014.igem.org/Team:Tongji/lab-archives"