Team:TU Darmstadt/Notebook/Labjournal/K1497018

From 2014.igem.org

(Difference between revisions)
 
(2 intermediate revisions not shown)
Line 7: Line 7:
<div id="leftNavi" class="grid_5">
<div id="leftNavi" class="grid_5">
<nav>
<nav>
-
<ul class="menu"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt" >Home</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Project" >Project</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results" >Results</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/PolicyandPractices" >Policy & Practices</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Achievements" >Achievements</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook" >Notebook</a><ul class="menu2"><li class="active first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal" >Labjournal</a><ul class="menu3"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497000" >K1497000</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497001" >K1497001</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497002" >K1497002</a></li><li ><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497003" >K1497003</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497004" >K1497004</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497005" >K1497005</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497006" >K1497006</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497007" >K1497007</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497008" >K1497008</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497009" >K1497009</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497010" >K1497010</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497011" >K1497011</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497012" >K1497012</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497013" >K1497013</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497014" >K1497014</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497015" >K1497015</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497016" >K1497016</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497017" >K1497017</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497018" >K1497018</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497019" >K1497019</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497020" >K1497020</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497021" >K1497021</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497022" >K1497022</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497023" >K1497023</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497024" >K1497024/5/6</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497027" >K1497027/28/29/30</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497031" >K1497031</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497032" >K1497032</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497033" >K1497033</a></li></ul></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials" >Materials</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods" >Methods</a></li></ul></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Team" >Team</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Gallery" >Gallery</a></li></ul>
+
<ul class="menu"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt" >Home</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Project" >Project</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results" >Results</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/PolicyandPractices" >Policy & Practices</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Achievements" >Achievements</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook" >Notebook</a><ul class="menu2"><li class="active first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal" >Labjournal</a><ul class="menu3"><li class="first"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497000" >K1497000</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497001" >K1497001</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497002" >K1497002</a></li><li ><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497003" >K1497003</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497004" >K1497004</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497005" >K1497005</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497006" >K1497006</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497007" >K1497007</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497008" >K1497008</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497009" >K1497009</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497010" >K1497010</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497011" >K1497011</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497012" >K1497012</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497013" >K1497013</a></li><li ><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497014" >K1497014</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497015" >K1497015</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497016" >K1497016</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497017" >K1497017</a></li><li class="active"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497018" >K1497018</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497019" >K1497019</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497020" >K1497020</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497021" >K1497021</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497022" >K1497022</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497023" >K1497023</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497024" >K1497024/5/6</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497027" >K1497027/28/29/30</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497031" >K1497031</a></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497032" >K1497032</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Labjournal/K1497033" >K1497033</a></li></ul></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Materials" >Materials</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Notebook/Methods" >Methods</a></li></ul></li><li><a href="https://2014.igem.org/Team:TU_Darmstadt/Team" >Team</a></li><li class="last"><a href="https://2014.igem.org/Team:TU_Darmstadt/Gallery" >Gallery</a></li></ul>
</nav>
</nav>
</div>
</div>
<div id="wikicontent" class="grid_19">
<div id="wikicontent" class="grid_19">
-
<!--TYPO3SEARCH_begin--><div id="c111" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">K1497014 - Inducible pelargonidin operon</h1></div><div class="csc-textpic-text"><p>
+
<!--TYPO3SEARCH_begin--><div id="c111" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">K1497018 - Composite part of F3H and DFR with strong RBS</h1></div><div class="csc-textpic-text"><p>
-
To generate two pelargonidin producing operons, we used the constructed parts: K1497009, K14970013, K14970011 and K1497002. We assembled all parts by using BioBrick standard assembly 10. All procedures were performed as described in methods section. The expression of pelargonidin was performed according to Yan et al., (2007). An overnight LB culture was used to inoculate an expression-culture. After the induction with 1mM Isopropyl-?-D-thiogalactopyranosid (IPTG) E.coli BL21 (DE3) cells were transferred into M9 media and fermented for 48h at 37°C in presents of 0.1mM naringenin. 
+
To generate the composite part of F3H and DFR both with strong RBS we used the constructed parts: <a href="http://parts.igem.org/Part:BBa_K1497012" title="Opens internal link in current window" class="internal-link"> BBa_K1497012</a> and <a href="http://parts.igem.org/Part:BBa_K1497013" title="Opens internal link in current window" class="internal-link"> BBa_K1497013</a>. We assembled all parts in <a href="http://parts.igem.org/Part:BBa_K1497018" title="Opens internal link in current window" class="internal-link"> BBa_K1497018</a> by using BioBrick standard assembly 10. All procedures were performed as described in our methods section.
</p></div></div><!--TYPO3SEARCH_end-->
</p></div></div><!--TYPO3SEARCH_end-->
</div>
</div>
 +
</html>
</html>

Latest revision as of 03:00, 18 October 2014

Home