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Team:Tuebingen/Notebook/Protocols
From 2014.igem.org
(Difference between revisions)
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<li><a href="/Team:Tuebingen/Notebook/Protocols/OligoAnnealing">Oligo Annealing</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/OligoAnnealing">Oligo Annealing</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/PCR">PCR</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/PCR">PCR</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/SyntheticPeptides">Peptidesynthesis</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/miniprep">Preparation of plasmids on a small scale</li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/miniprep">Preparation of plasmids on a small scale</li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/restriction">Preparative restriction digests of BioBricks and plasmids </a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/restriction">Preparative restriction digests of BioBricks and plasmids </a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/SDS-PAGE">SDS-PAGE</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/SDS-PAGE">SDS-PAGE</a></li> | ||
| - | |||
<li><a href="/Team:Tuebingen/Notebook/Protocols/TAE_buffer">TAE buffer</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/TAE_buffer">TAE buffer</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/TBE_buffer">TBE buffer</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/TBE_buffer">TBE buffer</a></li> | ||
Revision as of 21:24, 17 October 2014
Protocols
- Creating competent Escherichia coli cells
- Agarose gel electrophoresis
- Agarose gel extraction
- DNA-Ligation
- Laemmli buffer
- LB agar
- LB medium
- LB plates
- Loading buffer
- Mutagenesis PCR
- Ni NTA buffers
- Ni NTA purification
- Oligo Annealing
- PCR
- Peptidesynthesis
- Preparation of plasmids on a small scale
- Preparative restriction digests of BioBricks and plasmids
- SDS-PAGE
- TAE buffer
- TBE buffer
- Three part assembly
- Transformation of E. coli
