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Team:Tuebingen/Notebook/Protocols/Ni NTA Buffers
From 2014.igem.org
Protocols
Ni NA buffers
Lysis buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 10 mM | Imidazole |
Washing buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 20 mM | Imidazole |
Elution buffer (pH = 8)
| 50 mM | NaH2PO4 |
| 300 mM | NaCl |
| 250 mM | Imidazole |
