Team:Tuebingen/Notebook/Protocols/SyntheticPeptides
From 2014.igem.org
Protocols
Synthetic Peptides
Peptides were obtained by solid-phase synthesis using the Fmoc strategy. Cleavable TentaGel® R RAM from Rapp Polymers was used for synthesis. Deprotection of the Fmoc-protected amino group on the surface of the TentaGel®-Resin was performed with 20 % piperidine in dimethylformamide (2x15 min).
![](https://static.igem.org/mediawiki/2014/2/29/Tuebingen14_Fmoc.jpg)
Figure 1: Mechanism for the deprotection of Fmoc-protected amines
Cysteine was activated with HBTU (0.97 eq.) and added twice in 5-fold excess. The product was washed 5 times in DMF and 2 times in dichloromethane (DCM). Deprotection of the Fmoc-protected amino group of the coupled cysteine was performed like the deprotection of the TentaGel®-Resin. HBTU (0.97 eq.) activated lysine (Fmoc-Lys(Mtt)-OH) was coupled twice in 3-fold excess, with the second coupling taking place overnight. The 4-methyltrityl (Mtt) protection group was removed by washing the resin with 1 % trifluoroacetic acid (TFA) in DCM with gravity flow. Carboxyfluorescein was activated with HBTU (0.97 eq.) and coupled twice to the free ε-amino group of the lysine, whereas the second coupling occurred overnight. After extensive washing and deprotection of the α-amino group, the synthesis of the tag-specific region was performed automatically using an Invatis AG ResPep System. Fmoc-Ala-OH, Fmoc-Asn(Trt)-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Gly-OH, Fmoc-His(Trt)-OH, Fmoc-Ile-OH, Fmoc-Lys(Boc)-OH, Fmoc-Met-OH, Fmoc-Phe-OH, Fmoc-Pro-OH, Fmoc-Thr(tBu)-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Val-OH and Fmoc-Ahx-OH were used for this purpose. N-methyl-2-pyrrolidone (NMP) was added to the solvents increasing the solubility of the amino acids and prevented precipitation in the machine.
![](https://static.igem.org/mediawiki/2014/b/bf/Tuebingen14-HBTU.jpg)
Figure 2: Mechanism of the coupling reaction
The processed product was cleaved from the resin and unprotected from their side chain protection groups using a cleavage solution consisting of 670 mg of phenol, 450 μl of water, 450 μl of triisopropylsilyl (TIPS) and 9 ml of TFA. The resulting peptides were precipitated in cold diethyl ether and lyophilized overnight. Purification was performed by high-performance liquid chromatography (HPLC) and the resulting product was checked by mass spectrometry using electrospray ionization (ESI).