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Team:Tuebingen/Notebook/Protocols
From 2014.igem.org
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<li><a href="/Team:Tuebingen/Notebook/Protocols/mutagenesis_PCR">Mutagenesis PCR</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/mutagenesis_PCR">Mutagenesis PCR</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/Ni_NTA_Buffers">Ni NTA buffers</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/Ni_NTA_Buffers">Ni NTA buffers</a></li> | ||
| - | <li><a href="/Team:Tuebingen/Notebook/Protocols/NiNTA">Ni NTA</a></li> | + | <li><a href="/Team:Tuebingen/Notebook/Protocols/NiNTA">Ni NTA purification</a></li> |
<li><a href="/Team:Tuebingen/Notebook/Protocols/OligoAnnealing">Oligo Annealing</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/OligoAnnealing">Oligo Annealing</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/PCR">PCR</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/PCR">PCR</a></li> | ||
Revision as of 16:58, 17 October 2014
Protocols
- Creating competent Escherichia coli cells
- Agarose gel electrophoresis
- Agarose gel extraction
- DNA-Ligation
- Laemmli buffer
- LB agar
- LB medium
- LB plates
- Loading buffer
- Mutagenesis PCR
- Ni NTA buffers
- Ni NTA purification
- Oligo Annealing
- PCR
- Preparation of plasmids on a small scale
- Preparative restriction digests of BioBricks and plasmids
- SDS-PAGE
- Creation of synthetic peptides
- TAE buffer
- TBE buffer
- Three part assembly
- Transformation of E. coli
