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Team:TU Darmstadt/Notebook/Labjournal/K1497015
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| + | <!--TYPO3SEARCH_begin--><div id="c111" class="csc-default"><div class="csc-header csc-header-n1"><h1 class="csc-firstHeader">K1497014 - Inducible pelargonidin operon</h1></div><div class="csc-textpic-text"><p> | ||
| + | To generate two pelargonidin producing operons, we used the constructed parts: K1497009, K14970013, K14970011 and K1497002. We assembled all parts by using BioBrick standard assembly 10. All procedures were performed as described in methods section. The expression of pelargonidin was performed according to Yan et al., (2007). An overnight LB culture was used to inoculate an expression-culture. After the induction with 1mM Isopropyl-?-D-thiogalactopyranosid (IPTG) E.coli BL21 (DE3) cells were transferred into M9 media and fermented for 48h at 37°C in presents of 0.1mM naringenin. | ||
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Latest revision as of 16:39, 17 October 2014
K1497014 - Inducible pelargonidin operon
To generate two pelargonidin producing operons, we used the constructed parts: K1497009, K14970013, K14970011 and K1497002. We assembled all parts by using BioBrick standard assembly 10. All procedures were performed as described in methods section. The expression of pelargonidin was performed according to Yan et al., (2007). An overnight LB culture was used to inoculate an expression-culture. After the induction with 1mM Isopropyl-?-D-thiogalactopyranosid (IPTG) E.coli BL21 (DE3) cells were transferred into M9 media and fermented for 48h at 37°C in presents of 0.1mM naringenin.
