The PCR -amplification of the single domains of the scaffold protein yielded strong and clear bands migrating at the expected heights (see figuree 1). SH3 has a full length of 286 bp, PDZ of 400 bp and GBD of 354 bp. The digested fragments were cloned into the vector pSB1C3. Successful insertion of the SH3 and PDZ domain was verified by a colony PCR ( see figure 2). All three tested colonies were positive. However, all bands of the GBD domain differed from each other. Only one of three tested PCR -reactions showed a weak intensity at the expected height. Verification through sequencing of the generated vectors was omitted, since the three domains were initially amplified from positive sequenced template plasmids. The PCR-products were cloned into the vector pSB1C3 and E. coli Top10 cells were transformed with the constructs and tested via colongy-PCR.