Team:Tongji/safety-zh
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+ | <li class=""> | ||
+ | <a href="https://2014.igem.org/Team:Tongji">iGEM 同济 2014</a> | ||
+ | </li> | ||
+ | <li class=""> | ||
+ | <a href="https://2014.igem.org/Team:Tongji/project">项目介绍</a> | ||
+ | </li> | ||
+ | <li class=""> | ||
+ | <a href="https://2014.igem.org/Team:Tongji/human-practice">对外事务所</a> | ||
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+ | <a href="https://2014.igem.org/Team:Tongji/lab-archives">实验室档案馆</a> | ||
+ | </li> | ||
+ | <li class="active"> | ||
+ | <a href="https://2014.igem.org/Team:Tongji/safety">安全保障</a> | ||
+ | </li> | ||
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+ | <a href="https://2014.igem.org/Team:Tongji/honors-room">荣誉室</a> | ||
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+ | <li ><a href="https://2014.igem.org/Team:Tongji" target="_blank">English</a></li> | ||
+ | <li role="presentation"><a href="https://2014.igem.org/Team:Tongji-zh">中文</a></li> | ||
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+ | <!-- Subhead | ||
+ | ================================================== --> | ||
+ | <header class="jumbotron subhead" id="overview"> | ||
+ | <div class="container"> | ||
+ | <h1>安全保障</h1> | ||
+ | <p class="lead"></p> | ||
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+ | <li><a href="#download-bootstrap"><i class="icon-chevron-right"></i> 概述</a></li> | ||
+ | <li><a href="#file-structure"><i class="icon-chevron-right"></i> 问答环节</a></li> | ||
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+ | <h1>概述</h1> | ||
+ | </div> | ||
+ | <p class="lead">我们所有队员在项目开始前都被进行过实验室安全教育。我们参加过的教育项目可以在<a onclick=window.parent.location.href="http://shbc.tongji.edu.cn:8090/net/foreground/detail.jsp?wid=201309290004447718">这里</a>找到。我们的项目被学校官方批准。</p> | ||
+ | |||
+ | <p>张仕晟于2014年六月23日向iGEM提交了实验室表格。</p> | ||
+ | <p>张仕晟于2014年7月21日向iGEM提交了预审版本的安全表格。</p> | ||
+ | |||
+ | |||
+ | </section> | ||
+ | |||
+ | |||
+ | |||
+ | <!-- File structure | ||
+ | ================================================== --> | ||
+ | <section id="file-structure"> | ||
+ | <div class="page-header"> | ||
+ | <h1>问答环节</h1> | ||
+ | </div> | ||
+ | <h3>问题一:请问你如何保证你实验室里的微生物不会对实验人员以及环境造成危害?</h3> | ||
+ | <p class="lead">回答一:在我们项目实验里,我们使用<em>E. coli</em> DH5α 或 BL21(DE3) 作为受体微生物,使用 <em>Clostridium stercorarium</em> (DSM 8532) 作为供体微生物。他们都是S1级别的微生物。此外,<em>Clostridium stercorarium</em> (DSM 8532) 是个严格厌氧菌,一般认为对环境和实验人员都是没有危害的(信息来自<a onclick=window.parent.location.href="http://www.dsmz.de/catalogues/details/culture/DSM-8532.html?tx_dsmzresources_pi5%5BreturnPid%5D=304">这里</a>)。而且我们在丢弃曾接触细菌的材料前,都会进行灭菌,从而将风险降为零。</p> | ||
+ | <br> | ||
+ | <h3>问题二:你们在实验中有无使用有毒试剂?你们有什么保护措施?</h3> | ||
+ | <p class="lead">回答二:对于分子生物学实验,大多数实验室无法避免使用EB和丙烯酰胺。我们能做的就是,严格按照实验室地操作规定,把伤害降到最小。在造纸环节中,我们使用了84消毒液,里面有次氯酸钠,可能听起来比较危险。但事实上,我们日常生活中用它来洗碟子甚至蔬菜,而且我们使用时戴手套,所以说肯定安全。</p> | ||
+ | <br> | ||
+ | <h3>问题三:既然你用来参与漂白的酶是取自微生物的,你怎么保证你造的纸是安全的?</h3> | ||
+ | <p class="lead">回答三:我们的酶是在细胞浆中合成并最终释放在周质空间里的,所以我们决定用渗透压法去提取酶。因此,抽取含有蛋白的上清后,菌体就没有了。所以,没有菌体会在纸浆里出现,所以造出来的纸是安全的。</p> | ||
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Revision as of 15:31, 13 October 2014
安全保障
概述
我们所有队员在项目开始前都被进行过实验室安全教育。我们参加过的教育项目可以在这里找到。我们的项目被学校官方批准。
张仕晟于2014年六月23日向iGEM提交了实验室表格。
张仕晟于2014年7月21日向iGEM提交了预审版本的安全表格。
问答环节
问题一:请问你如何保证你实验室里的微生物不会对实验人员以及环境造成危害?
回答一:在我们项目实验里,我们使用E. coli DH5α 或 BL21(DE3) 作为受体微生物,使用 Clostridium stercorarium (DSM 8532) 作为供体微生物。他们都是S1级别的微生物。此外,Clostridium stercorarium (DSM 8532) 是个严格厌氧菌,一般认为对环境和实验人员都是没有危害的(信息来自这里)。而且我们在丢弃曾接触细菌的材料前,都会进行灭菌,从而将风险降为零。
问题二:你们在实验中有无使用有毒试剂?你们有什么保护措施?
回答二:对于分子生物学实验,大多数实验室无法避免使用EB和丙烯酰胺。我们能做的就是,严格按照实验室地操作规定,把伤害降到最小。在造纸环节中,我们使用了84消毒液,里面有次氯酸钠,可能听起来比较危险。但事实上,我们日常生活中用它来洗碟子甚至蔬菜,而且我们使用时戴手套,所以说肯定安全。
问题三:既然你用来参与漂白的酶是取自微生物的,你怎么保证你造的纸是安全的?
回答三:我们的酶是在细胞浆中合成并最终释放在周质空间里的,所以我们决定用渗透压法去提取酶。因此,抽取含有蛋白的上清后,菌体就没有了。所以,没有菌体会在纸浆里出现,所以造出来的纸是安全的。